You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look under the microscope, what color will a Gram positive bacteria, like Staphylococcus aureus, be? Red/Pink Purple colorless
Q: After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in…
A: Introduction Gram staining, commonly known as Gram's method, is a staining technique that is used to…
Q: Which stain is most frequently used to distinguish differences between thecell walls of medically…
A: The cell walls of bacteria are made up of peptidoglycan- a layer of polysaccharide chains cross…
Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…
A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…
Q: If the iodine step in a Gram-staining procedure was omitted, what color would you expect…
A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…
Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…
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Q: hich Is The More Important Step In Gram Stain?
A: Hans Christian Gram invented the Gram stain in 1884. (Gram,1884).
Q: What does gram's iodine do for the stain? fixes the color to the outer layer of peptidoglycan…
A: Bacteria is a prokaryotic microorganism and it has a cell wall protecting the cell. Based on the…
Q: Which of the following places the steps of the Gram stain in the correct order? 1-Alcohol-acetone…
A: There are two types of bacteria, i.e gram-positive and gram-negative.
Q: Why is oil immersion necessary to see bacteria in the laboratory?
A: Oil immersion technique is used to increase the resolving power of microscope. Both the objective…
Q: Which of the microscope objectives is most satisfactory for studying bacteria? Why?
A: For studying bacteria , under the microscope , 100X objective oil immersion lens is generally used…
Q: You prepared another slide to visualize the bacteria under the oil immersion objective lens, but you…
A: In microscopy, oil immersion is a technique used to increase the resolving power of a microscope.…
Q: Examples of bacteria observe in the microscope using Gram stain, Ziehl-Neelsen, and Kinyoun Method.
A: Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen…
Q: In the Gram stain, gram-positive bacteria are dark purple for all the following reasons EXCEPT:…
A: Organisms can be provisionally identified by growing them on medium and looking at their shape and…
Q: What features of your Gram stain exemplify the Gram stain theory?
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…
A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…
Q: Describe why Gram negative bacteria stain pink.
A: Gram staining is the differential staining technique performed to differentiate the bacterial…
Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…
A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…
Q: The Gram Stain is able to stain bacterial cells differently depending upon the structure of their…
A: Bacterial cell wall is made up of peptidoglycan. it prevents the cell from bursting when it absorbs…
Q: When doing a gram stain in microbiology, one step can be eliminated and still allow distinction…
A: Please follow step 2 for detailed explanation.
Q: The picture shows bacteria (Escherichia coli) under a microscope Do you bacterial growth or no (…
A: Culture media are an integral part of microbiology lab where bacteria can be grown on different…
Q: Describe what happens at each step in the Gram stain.
A: BASIC INFORMATION GRAM STAIN It is a method of staining through which the bacterial species can be…
Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…
A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…
Q: Suppose you performed a Gram stain on a sample, from a pure culture of bacteria and you observed a…
A: Gram staining is a method that use dye to stain the bacteria and the color helps them to distinguish…
Q: If you prepared a sample of a specimen for light microscopy, stained it with the Gram stain, and…
A: Bacteria are unicellular prokaryotic microorganisms (nucleus is not enclosed in a nuclear membrane)…
Q: Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the…
A: The wet mount is the most basic method of preparation, in which the specimen is placed on the slide…
Q: microscope
A: Microscope helps to see the small things clearly
Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…
A: Gram staining is performed on the basis of differential characteristics of the cell wall in…
Q: Following a gram stain, you observe clear, glassy areas inside the bacterial cells. What would you…
A: Gram staining is a technique that is used to differentiate between bacterial cells on the basis of…
Q: Why do cheek cells stain red with the gram stain? What is the big red circle in the middle of the…
A: Bacteria that have a thick layer of a substance called "peptidoglycan" in their cell wall will get a…
Q: On the basis of the reaction in the Gram stain, bacteria can be divided into which two major groups?
A: The bacteria are the part of prokaryotes that are included in the kingdom Monera. Bacteria are rich…
Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…
A: Gram staining helps in differentiation between gram positive and gram negative bacteria. Procedure…
Q: When performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal…
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Q: An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and…
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Q: A novice microbiology student incorrectly explains that immersion oil increases the magnification of…
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Q: Why isn’t the Gram stain used on acid-fast bacteria? If you did Gram stain acid-fast bacteria, what…
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Q: What color would the bacterial cells have when viewed theoretically under the microscope after the…
A: Gram staining is a age old method to classify bacteria according to the composition of their cell…
Q: You use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider…
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Q: You partner stained bacterial cells and saw only the background and not the actual cell was stained.…
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Q: 400x 1000x What is the shape of bacteria under a microscope, what color is there, and is there Gram…
A: INTRODUCTION Gram staining is a differential staining used to differentiate bacteria into gram…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: What color would the bacterial cells have when viewed microscopically after the following…
A: Gram staining is a technique proposed by Christian Gram to distinguish Gram positive and negative…
Q: What type of stain is the Gram stain and what does it rely on for meaningful results
A:
Q: What color are Gram negative cells after decolorizing with ethanol? What is the best age for your…
A: Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…
Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…
A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…
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Q: What is the difference between gram positive and gram negative bacteria? Describe how we use this to…
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Q: Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram…
A: The Gram staining procedure in bacteria is depends on the chemical properties of cell wall. In Gram…
You accidentally switch the Crystal violet and Safranin steps during your
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Purple
colorless
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- Janet is doing a Gram stain on a mixed culture that is known to contain both Gram positive and Gram negative bacteria. After finishing, she realizes she mixed up the safranin and alcohol steps. What is likely to be observed when she views the slide under the microscope? All cells will appear purple, regardless of whether they are actually Gram negative or Gram positive. All cells will appear pink, regardless of whether they are actually Gram negative or Gram positive. Gram negative cells will be clear, Gram positive cells will be purple. Gram positive cells will be clear, Gram negative cells will be pink. Cells will appear clear, regardless of whether they are actually Gram negative or Gram positive.You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purpleWhen performing a Gram stain on a Gram-negative bacteria, why does the bacteria stain pink? Crystal violet stain binds to iodine and remains in the bacterial cell wall because of the thick peptidoglycan layer. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. The alcohol wash removes crystal violet and iodine from the bacteria. Crystal violet stain binds to iodine and remains in the bacteria cell wall because of the thick peptidoglycan layer AND Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink. Safranin counterstain gets in the bacteria’s cell wall to stain the cell pink AND The alcohol wash removes crystal violet and iodine from the bacteria.
- The bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?After conducting the Gram staining procedures and you observe nothing (you didn’t see anything) in your slide under the microscope, what could be the mistake you have done? What can you observe when you view a Gram stained-mixture of bacterial specimen?What is the difference between gram positive and gram negative bacteria? Describe how we use this to our advantage in the gram staining process. Hint: Gram positive versus Gram negative!!!
- Imagine that you are Gram staining a mixed culture of both gram-positive and gram-negative bacteria. If you omitted the iodine (mordant) step in the procedure, what colors would the bacteria on your slide appear to be? ► The gram-positive bacteria would be purple, and the gram-negative would be pink All of the bacteria would look purple All of the bacteria would look pink The bacteria would not be stained at all (clear)An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? No acid-fast bacteria are present on the slide Methylene blue was not used Carbolfuchsin was not used Acid-alcohol was not usedYou receive a bacterial culture in the lab and your job is to figure out information which will be helpful. You are only allowed to use one of the staining methods we practiced. Which one would be the best option? Why? And what will this information help you determine? Explain.
- What color are Gram negative cells after decolorizing with ethanol? What is the best age for your culture when performing a Gram stain? Why? Why is Gram staining considered a differential staining process? What are the primary and counterstain? What is the purpose for using a counterstain? What are some reasons a Gram positive cell might appear Gram negative? What color would a Gram negative cell be if you forgot to wash with ethanol and why? What color would a Gram negative cell be if you forgot to stain it with safranin and why?If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? No; the clumps will wash away later. Yes; the clumps will hide the individual bacteria. The clumps will not stain. Yes; the clumps of bacterial cells may retain the stains and lead to incorrect data being collected. SubmitRequest AnswerAll of the following are correctly matched and can be found in bacteria EXCEPT: None of the other four answers (all are correctly matched and can be found in bacteria) Nucleus membrane-bound organelle containing multiple chromosomes Ribosomes - site of protein synthesis Cell wall contains peptidoglycan Cell (plasma) membrane - encloses cytoplasm; phospholipid bilayer with proteins