You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, Please explain so I can understand in the answer What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s repressor?

You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, Please explain so I can understand in the answer What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s repressor?

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter14: Gene Regulation

Section: Chapter Questions

Problem 11TYU

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You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions I really need a in depth epxplantion for the queshtions to make sure i understand the answers a.What effect does lactose have on the bacterial cell’s lac operon? b.What does the absence of glucose do to the bacterial cell? c.You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? d.What kind of regulation does lactose provide to an operon’s repressor?
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence?
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions Which operons would be functional under these conditions? 2. What repressors would be made? (NOTE: name the repressors using their gene names) 3.Which repressor(s) would be made in the inactive form? 4.Which repressor(s) would be made in the active form? 5.Which repressor(s) under these conditions can bind the operator sequence? 6.Which repressor(s)under these conditions cannot bind the operator sequence?
You are growing E. coli in a laboratory in order to study their operons. The growth media you are using contains lactose, no glucose and no tryptophan. Using your knowledge of operons and their regulation, answer the following questions using the dropdown menus provided in Canvas. Which operons would be functional under these conditions? What repressors would be made? (NOTE: name the repressors using their gene names) Which repressor(s) would be made in the inactive form? Which repressor(s) would be made in the active form? Which repressor(s) under these conditions can bind the operator sequence? Which repressor(s)under these conditions cannot bind the operator sequence? What effect does lactose have on the bacterial cell’s lac operon? What does the absence of glucose do to the bacterial cell? You now add tryptophan to the cell. What would happen to the bacterial cell and its trp operon? What kind of regulation does lactose provide to an operon’s…
Describe operons in your own words, as if you were explaining what an operon is to someone who knew about DNA and cells etc but did not know what an operon was. Here are some things you can discuss: Are operons controlling transcription or translation? Which organisms are they found in? What do the genes in an operon have in common with each other?
If glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
What are the effects of the following conditions on Lac operon of bacteria? Do not forget to mention about the role of repressor, activator, RNA polymerase in each case! Glucose is absent and lactose is present Glucose is present and lactose is present Glucose is present and lactose is absent
If glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?
The transcription of many bacterial genes relies on functional groups called operons, such as the tryptophan operon. What is an operon?
The trp operon in E. coli encodes enzymes essential for the biosynthesis of tryptophan. In the operon, trpR gene encodes the repressor. A trpR repressor cannot bind tryptophan. The trpO is the operator. trpA encodes the enzyme tryptophan synthetase. For the enzyme (A), indicate with a + or-whether or not it is made when there is tryptophan and when there is no tryptophan. Without Tryptophan With Tryptophan R'O A /R* O' A R'O' A/R* Oʻ A R*OA/R' O A
Use the blanks on the left hand side to put the following statements in order (use numbers or letters to designate the order). All of the statements refer to the diauxic (sequential) growth of a bacterium on the carbon substrates glucose and lactose (see textbook Fig 4.11 on diauxic growth). Remember, the lac operon contains the genes necessary for the lactose catabolism. The glucose substrate is completely consumed, at which time the repressor protein on the lac operon unbinds from the DNA molecule. The lactose substrate is catabolized by the active B-galactosidase enzyme. Glucose, as the preferred carbon substrate, is first consumed by catabolic enzymes that are constitutive (always present). After completion of translation, the B-galactosidase enzyme undergoes folding to form into an active enzyme that can breakdown lactose into its constituent monosaccharides. The messenger RNA code from the lac operon is translated by ribosomal RNAS to form lac-related enzymes, such as…
Which of the following lac operon genotypes would allow for functional versions of all the structural enzymes of the lac operon to be expressed constitutively even in the absence of lactose? Group of answer choices I+ O+ Z+ Y+ A+ I- O+ Z- Y- A- I+ OC Z+ Y+ A+ IS O+ Z+ Y+ A+ I+ O+ Z- Y+ A+