You are making ampicillin plates. Before pouring the plates, you add 2 mL of 10 mg/mL to the 400-ml bottle of LB agar that you use to pour the plates. What is the final concentration of the ampicillin in the plates? Express your answer as µg/mL. Show your work.

You are making ampicillin plates. Before pouring the plates, you add 2 mL of 10 mg/mL to the 400-ml bottle of LB agar that you use to pour the plates. What is the final concentration of the ampicillin in the plates? Express your answer as µg/mL. Show your work.

Chemical Principles in the Laboratory

11th Edition

ISBN:9781305264434

Author:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Publisher:Emil Slowinski, Wayne C. Wolsey, Robert Rossi

Chapter43: Analysis For Vitamin C

Section: Chapter Questions

Problem 3ASA

See similar textbooks

Related questions

Q: Rate constants for decomposition of dinitrogen pentoxide are 3.7 x105 s¹ at 25.0 °C and 3.4 x10³ s¹…

Q: Consider the following substances: Lead (II) Carbonate, PbCO3 KSP = 7.40 x10-14 Magnesium Carbonate,…

Q: Consider the following reaction: CO2(g)+CCl4(g)⇌2COCl2(g)CO2(�)+CCl4(�)⇌2COCl2(�). Calculate…

Q: When a 50. mL sample of C₂H5OH is mixed with a 50. mL sample of H₂O, the resulting mixture has a…

Q: Why does this equation for the electron ratio have two radii (R and r)?

A: In the electron ratio equation, the two radii (R and r) refer to different aspects of the system…

Q: Give formulas and the geometry around the central metal ion corresponding to the following; a)…

Q: Br .. Н Н Cl ОН

A: The Newman projection and the wedge-dash projection are two commonly used methods for depicting the…

Q: A 1M solution of Cu(NO3)2 is placed in a beaker with a strip of Cu metal. A 1M solution of SnSO4 is…

Q: The molar mass of nicotine C10H14Nx is 162 g/mol. What is the value of x?

Q: Shown below is the C13 NMR spectrum of one of the three isomeric dichlorobenzenes. Is the compound=…

A: The question can be solved by closely observing the number of peaks in the given 13C-NMR spectrum.…

Q: The image below depicts the formation of what type of solution? Solvent- solvent Step 1 Irrelevant…

A: Solutions- Types of solutions. Different types of solutions.

Q: Which statement is correct concerning the relative stabilities of the two conformations, A and B,…

Q: Propose structures for compounds G-l: OH OH cono H₂SO 60-65°C G (C₂HS₂0₂) conc HNO₂ cono H₂SO H…

A: Resorcinol, when subjected to sulphonation leads to the formation of benzene sulfonic acid. This is…

Q: How do you know from a chemical formula if it's molecular or covalent? For example, SO2 is a…

A: Introduction A molecular solid is a type of solid state material composed of molecules held together…

Q: 4.15) The bond angles around all of the carbons in 2-methylbutane are the same because all of the…

A: To find :- ABE classification of carbons in the given molecule bond angles around carbons in the…

Q: what do you think the UV-vis spectrum of a sample that was not clear (there was particulare in the…

A: When analyzing a sample using UV-vis spectroscopy, the presence of particulates in the solution or…

Q: A certain reaction is first order in H₂ and first order in I₂. Use this information to complete the…

Q: Macmillan Learning Complete and balance the equation for this single-displacement reaction. Phases…

Q: Supply the missing starting compound. A (1) CH₂MgBr + (2) NH, CH3 CH3CHCH₂C-OH CH3 CH3

Q: 1. When the following equation is balanced, the coefficient of Al2O3 is Al203 (s) + C (s) + Cl2 (g)…

Q: Choose the correct answer to fill in each blank. If a compound dissolves in water and the resulting…

A: These statements relate to the solubility and electrical conductivity of chemical compounds, and the…

Q: The reaction in Example 16.5 between CO and H2 is carried out at a different temperature with…

A: Given : Initial concentration of CO = 0.27 M Initial concentration of H2 = 0.49 M Concentration…

Q: on 5 of 27 Macmillan Learning Draw the structure of an alkane or cycloalkane that has more than…

A: We have been asked to draw the structure of an alkane or a cycloalkane that has more than three but…

Q: (a) Distinguish briefly between Solid, liquid and gas (b) State the difference between Evaporation,…

Q: In the mass spectrum of bromoform, CHBr3, molecular ion peaks appear at m/z 250, 252, 254, and 256?…

A: Given that In the mass spectrum of bromoform, CHBr3 molecular ion peaks appear at m/z: 250, 252,…

Q: 6) 7) 8) NH3 + H₂O + H₂SO4 + 12 → SO3 → Group 6 N₂16 + All Groups NH,OH → H₂SO4 H₂ Page 2 / 2 H₂O +…

Q: Decribe the working mechanism of a Cu-Zn cell as illustrated below. Zn anode Switch NO₂ NO₂ 1.10…

A: Galvanic cell - it is spontaneous process in which chemical energy is converted into electric energy

Q: A chemical reaction produces formaldehyde, with a chemical formula of CH2O. Carbon is in Group 4A,…

A: A molecule of formaldehyde contains covalent bonds formed by the sharing of valence electrons…

Q: The gas phase decomposition of hydrogen iodide at 700 K HI(g) → H₂(g) + ½/1₂ (9) is second order in…

A: We have been given initial concentration, concentration at time (t) and rate constant. We have been…

Q: Ⓒ Macmillan Learning A sample of hydrogen sulfide, H₂S, has a mass of 51.00 g. Calculate the number…

A: Mole concept: The number of molecules or atoms present in the one mole of the substance is equal to…

Q: Rank the following dienes in order of increasing reaction rate in a Diels- Alder Reaction (5 =…

A: We have been given five dienes and we have been asked to rank the given five dienes in order of…

Q: Which type of electromagnetic radiation has the shortest wavelength? O X rays Radio waves O Ultra…

A: X rays has the shortest wavelength means they have higher frequency. The order of increasing…

Q: Organic chemistry question I. Write an equation for the proton transfer reaction that occurs when…

Q: 4.55) In which of the following pairs of species can an ion-dipole interaction occur? a) H₂O and H₂O…

A: Given species: a) H2O and H2O b) H2O and CO2 c) Mg2+ and H2O d) Mg2+ and CO2 We have to find the…

Q: In the provided reaction profile, the black line represents the pathway of the reaction of A and B…

A: The catalyst is a chemical species that increases the rate of a chemical reaction and it does not…

Q: Use the orbital diagrams to explain why the first ionization energy of nitrogen is greater than the…

A: Ionization energy is the minimum energy required to remove the most loosely held electron or highest…

Q: 14. A CHEM 106 student made a 55.0 % by mass sodium sulfate aqueous solution at 50 °C (Assume the…

A: Solutions- The given graph shows the solubility of all substances. we have to find the solubility of…

Q: 3) Which of these structures contain aromatic groups? If there is an aromatic group, write the…

A: 1. Compound is said to be aromatic if it is cyclic, planar, has conjugation and has (4n+2) π…

Q: Question 2- Provide the structure of the missing starting material 1)Cul 2 equiv. ? 2) Br

A: Given missing reagents

Q: Identify which changes in the list below will cause the system at equilibrium to shift towards…

A: Le Chatelier's principle: This principle is used to predict the effect of a change in conditions on…

Q: Sample Jones Test 1 HO A 2345 2 4 CH3 Blue-green precipitates No reaction No reaction No reaction…

Q: How many kinds of chemically non-equivalent hydrogens are there in each of the following compounds?…

A: Chemically non-equivalent hydrogens means the types of hydrogens which are present at different…

Q: sample contains 52.0 g of elemental phosphorus, P4. Determine the number of moles of P4 and the…

A: Given , sample contains 52.0 g of elemental phosphorus, P4. To Determine the number of moles of P4…

Q: A sample of NOCl decomposes according to the following equation: 2 NOCl(g) 2 NO(g) + 1 Cl2(g)…

A: Answer :

Q: At a certain temperature K = 1.10×103 for the reaction: Fe3+(aq) + SCN-(aq) FeSCN2+(aq)…

Q: d) i) Mark all five stereocentres in this morphine derivative: ii) Identify as S or R the chiral…

A: The R and S configuration of a chiral molecule can be determined by assigning priorities to the four…

Q: An empty steel container is filled with 0.370 atm of A and 0.370 atm c B. The system is allowed to…

Q: b) i) The Parkinson's disease drug Levodopa is shown. Which one-letter stereodescriptor (S or R)…

A: According to Cahn-Ingold-Prelog rule- 1) More atomic number having more priority. 2) If first atom…

Q: Write a rate equation, showing the dependence of rate on reactant concentrations, for each of the…

A: Answer: Elementary reaction is a single step reaction in which reactants present in the balanced…

Q: carrying out Part 1 of this experiment, assume an excess of HCl(aq) reacts with 0.426 g of Mg(s). If…

Concept explainers

Environmental Pollution

Environmental pollution occurs due to some unwanted alteration in the surroundings which results in unfavorable effects on all organisms of that surrounding.

Question

5. You are making ampicillin plates. Before pouring the plates, you add 2 mL of 10 mg/mL to the
400-ml bottle of LB agar that you use to pour the plates. What is the final concentration of the
ampicillin in the plates? Express your answer as ug/mL. Show your work.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Step 3

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 3 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, chemistry and related others by exploring similar questions and additional content below.

Similar questions

50 mL of stock solution were taken and added to flask. Then 50 mL of Di water were added to the flask. This is solution 1. Then 50mL of solution 1 were taken and added to a flask and 50 mL of Di water were added to the flask. This is solution 2. Then 50mL of solution 2 were taken and added to a flask and 50 mL of Di water were added to the flask. This is solution 3. Then 50mL of solution 3 were taken and added to a flask and 50 mL of Di water were added to the flask. This is solution 4. Find the concetrations of each solution. Information of stock solution - molar mass- 534.3g/mole 0.587g in MilliQ
Table 1: Temp vs. solubility data of ammonium chloride. Test tube g NH4Cl/10 mL H2O Crystallization temp. (ºC) 1 1.0 g/10 mL H2O 10 oC 2 1.8 g/10 mL H2O 20 oC 3 2.9 g/10 mL H2O 40 oC 4 3.8 g/10 mL H2O 65 oC 5 4.4 g/10 mL H2O 95 oC Use the table to plot a solubility curve for ammonium chloride on the graph provided below. For the best fit line, use a smooth curve. "Mass of Solute per 100 mL of H2O," should be written on the y-axis (use increments of 2g for every box). "Temperature (°C)" should be written on the x-axis (use increments of 10 °C for every 2 boxes).
You have a 20% stock of NaCl, a 30% stock of K2HPO4, 2.5 M NH4Cl and 10 mg/ml stock of ampicillin. a. How many grams of NaCl would you need to weigh out to make up 150 ml of a solution?b. You will be preparing 300 ml of media. You need to dilute this 20% stock of NaCl, the 30% stock of K2HPO4, the 2.5 M NH4 how much stocks you need to add. Fill in the table below. Stock Calculate Final Concentration needed ml added20% NaCl 1.5% ? 30% K2HPO4 2% ? 2.5 M NH4 Cl 0.1M ? 10 mg/ml ampicillin water 0.05 mg/ml ? water To 300 ml ?
15. You are given a series of 10 tubes. In the first is placed 9 milliliters of saline. In each of the others is placed 5 mL of saline. One milliliter of serum is added to the first tube. Five milliliters from this tube are transferred to the second tube, and 5 ml are transferred throughout the remaining tubes. Discard the last 5ml transferred. Give the following: a. Tube dilution for tubes 1 and 6 b. The solution dilution for tubes 1,4, and 7 c. The amount of serum present in tube #3 after transfer.
A student, trying to standardize NBS solution using a standard Vitamin C solution (220 mg/L), obtained data that was obviously incorrect. They realized their mistake when they noticed the standard Vitamin C solution was dated 8/04. What was their mistake and explain why?
A 1 mL sample of glycogen was calculated to contain 21 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCl. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 340 µg/mL (microgram/milliliter). i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1 mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample. ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample. iii) Calculate the purity of the glycogen used in the sample as % Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100 iv) state your answer in a complete sentence. Show your working out such that the marker can easily understand it.…
A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter). i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample. ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample. iii) Calculate the purity of the glycogen used in the sample as % Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100 iv) state your answer in a complete sentence.
A protocol calls for 1 liter of solution that is 1.5 % (w/v) NaCl, 5mM Tris (pH 8.0), 10% (w/v) Carnation Instant Milk (we really do use this in lab!), and 0.03% (v/v) Tween-20 detergent. How do you make this solution?
Materials Needed solid I2 solid CUSO4-5H20 food dye solid (NH4)2SO4 heavy metals waste container halogenated waste container non-halogenated waste container semi-micro test tubes and rack regular test tubes and rack squash pipettes acetone cyclohexane propan-2-ol Method Part A: Solubility of ionic and molecular solids 1. Place a small amount (about the size of 1 grain of rice, see picture) of copper sulfate into each of three DRY semi-micro test tubes. Add 20 drops of water to the first test tube and gently flick the test tube with your finger to ensure mixing. 2. Repeat step 1 using acetone in place of water as the solvent in the second test tube. 3. Repeat step 2 replacing acetone with cyclohexane in the third test tube. Hold the test tubes against a white background to compare the solubility of copper sulfate in the three solvents and record your results. Discard these mixtures into the heavy metals waste container in the fume cupboard. Once these test tubes have been emptied you…
Pretend you are making dilutions using a 20-mg/dL stock standard solution of uric acid. Complete the table to indicate preparation of various concentrations. Concentration (mg/dL) Stock Standard (mL) Distilled H2O Total Volume of Dilution (mL) 0 0.0 5.0 5.0 2.5 5.0 5.0 5.0 7.5 5.0 10.0 5.0 12.5 5.0 20.0 5.0 0.0 5.0
In the phosphate analysis of a wastewater sample, 30.00 mL of the original sample was taken and diluted with 120.00 mL deionized water. The diluted sample was found to have a phosphate content of 25.5 ppm. What is the phosphate content (in ppm) of the original wastewater sample?
2. An 88-pound patient will be receiving aztreonam 30 mg/kg (MAX 1 gram) in 100 mL D₁0W. MWaztreonam = 435.43 g/mol General Structure: aztreonam a. If you have aztreonam 40 mg/mL in stock, calculate the appropriate volume, in milliliters, of the stock solution the patient should receive. b. Fill in the values on the table.