You are purifying a protein for the first me and decide to analyze it on SDS PAGE. You run two lanes on the gel, one with DTT and one without DTT (DTT is a subs tute for Beta-mercaptoethanol that is less s nky) The lane with DTT yields 2 bands one at 30kD and one at 20kD while the one without yields a single band at approximately 50 kD. A. What does this result suggest about the overall protein structure? B. Sketch what may be occurring in this protein natively.
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
You are purifying a protein for the first me and decide to analyze it on SDS PAGE. You run two lanes on
the gel, one with DTT and one without DTT (DTT is a subs tute for Beta-mercaptoethanol that is less
s nky) The lane with DTT yields 2 bands one at 30kD and one at 20kD while the one without yields a
single band at approximately 50 kD.
A. What does this result suggest about the overall protein structure?
B. Sketch what may be occurring in this protein natively.
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