3.You are studying an enzyme in the laboratory that is only active between pH 5 and 8.You wish to study the activity in a buffer of pH 7. Pick the best buffer system formaking your buffer:Buffer systemTris/HCIAcetic acid/acetateLactic acid/lactateCarbonateBicarbonatePhosphatepka8.14.83.96.410.27.2

A buffer solution is a water solvent-based solution consisting of mixture of weak acid and its…

Answered: You are studying an enzyme in the…

Ebk:Nutrition & Diet Therapy

10th Edition

ISBN:9780357391747

Author:DEBRUYNE

Publisher:DEBRUYNE

Chapter16: Specialized Nutrition Support: Enteral And Parenteral Nutrition

Section: Chapter Questions

Problem 2CA

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You have been provided with stock solutions of: stock A: 0.06 M sodium pyrophosphate buffer pH 8.5 stock B: 3 M ethanol stock C: 0.015 M NAD+ stock D: milli Q water Determine the volume you will need of each solution to prepare a buffer of with a final volume of 60 mL containing 10 mM sodium pyrophosphate pH 8.5, 100 mM ethanol, 1 mM NAD+. i.e. volume of stock A = _________mL volume of stock B = _________mL volume of stock C = _________mL volume of stock D = _________mL Show your calculations to arrive at your answers.
You have a stock solution containing 1.5 mM of substrate in borate buffer pH 9.5. You transfer 1.25 mL of this stock into a cuvette and then add 250 µl of enzyme solution to initiate the reaction. What will be the final concentration of substrate in the cuvette following addition of the enzyme? 1.5 mM 1.25 mM 0.0075 mM 7.5 mM 1250 mM
You are asked to prepare HEPES buffered saline with the following constituents: НЕРES 10mM KCI 5mM NaCl 135mM CaCl2 1mM M9SO4 0.6mM Glucose 6mM You have previously made up 100 mM stock solutions of HEPES, KCI, CaCl2, and M9SO4 and a 1M stock of NaCl. The MW of glucose is 180.16 g/mol What volume of HEPES would you require to make up 400ml of buffer?
From the Henderson-Hasselbach equation, calculate the ratio of dihydrogen phosphate (H2PO4-) and monohydrogen phosphate (HPO4-2) components required to produce buffer solution with1. pH 6.22. pH 7.23. pH 8.2
you get an order of 5% amino acid 15% dextrose premixed parenteral nutrition solution, 2L at 83mls/hr. Your pharmacy technician tells you there is a manufacture's backorder on those. How many ml of 20% dextrose would you need to provide the same amount of dextrose in 24 hours?
An appropriate biochemical buffer should have charges in both its conjugate acid and conjugate base form. This avoids the presence of a neutral form of the buffer that might be able slowly leak into cells. Does unmodified dimethylpiperazine meet this criterion when buffering at pH 8? O Yes No What about buffering at pH 4? D Yes No
How much water must be added to 300 ml of a S M aqueeus selution of HCI in arder to obtain a feal conicentration of 2 M7 O A 750 mL OB. 450 mt OG 120 ml O D.400 mt. QUESTION 37 What are the imolecular formulas for sodium sulfate, methylene chłoride and sodium bicarbanate respectively? OA NazS04, CH3CI NAHCO, OB, Nazso3, CHCI3. Naco3 O. NazsO4, CCly, NaHCO3 O D. none of the answers OE. NapSo4, C2CH, Nazco3
A researcher is preparing a reaction mixture to test the activity of a protein. They combine the required reaction components, which contained in a final 100 ml reaction volume 200 mM NaCl, unknown concentrations of acetic acid and acetate anions and a total [H+] concentration of 790 ricromolar. Can you determine the pH of the solution? Provide the answer to one decimal place. Note: You may need to round the numbers to get the required answer. 100 Strips pH indicator strips non-bleeding pH pH 0-14 EM-Reagents Dip in-read while still moist. immerse in weakly-buffered solutions une there is no further color change (1-10 min) 3 7 5 6
1. Describe how you would go about making up the following solutions. a) 150ml GTE buffer (resuspension solution) containing, 20mM Tris pH 8, 5000µM EDTA, 1M Glucose
Another source of phosphorus in water is known as organic phosphorus which commonly finds its way into natural bodies of water from human and animal waste and food residues. If you wish to measure the phosphorus content due to organic matter in the water sample using the vanadomolybdophosphoric acid method, you must first "digest" the sample to convert the organic phosphorus to orthophosphate. O True O False
Choose the FALSE statement An indicator can be used to monitor a buffer range When pH equals PKa of weak acid, the buffer capacity is the greatest Polyprotic acids have multiple stoichiometric points in their titration curve At the mid-point of titration of weak acid by strong base, the pH of the solution -does not equal the pKa of the weak acid The moles of titrant equals moles of titrate
Tris NaCl buffer is known as?

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