You attempted to determine the number of bacteria in a culture using the Turbidimetry method with a spectrophotometer and obtained the following data: Sample Optical Density Uninoculated Media only 0.054 1:16 dilution of culture 0.412 1:8 dilution of culture 0.964 1:4 dilution of culture 1.815 1:2 dilution of culture 2.450 1:1 dilution of culture 2.860 Using the formula below: OD600 of 1.0 = 8 x 108 cells/ml

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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You attempted to determine the number of bacteria in a culture using the
Turbidimetry method with a spectrophotometer and obtained the following
data:
Sample
Optical Density
Uninoculated Media only
0.054
1:16 dilution of culture
0.412
1:8 dilution of culture
0.964
1:4 dilution of culture
1.815
1:2 dilution of culture
2.450
1:1 dilution of culture
2.860
Using the formula below:
OD 600 of 1.0 = 8 x 108 cells/ml
Transcribed Image Text:You attempted to determine the number of bacteria in a culture using the Turbidimetry method with a spectrophotometer and obtained the following data: Sample Optical Density Uninoculated Media only 0.054 1:16 dilution of culture 0.412 1:8 dilution of culture 0.964 1:4 dilution of culture 1.815 1:2 dilution of culture 2.450 1:1 dilution of culture 2.860 Using the formula below: OD 600 of 1.0 = 8 x 108 cells/ml
What is the best estimate of the number of cells in the original culture
tube?
O 2.45x108 cells/ml
9.64x108 cells/ml
3.83x10° cells/ml
O 6.17x10° cells/ml
O 2.24x109 cells/ml
O 4.12x1010 cells/ml
5.82x10° cells/ml
O 2.29x10° cells/ml
O Cannot be determined
5.32x108 cells/ml
Transcribed Image Text:What is the best estimate of the number of cells in the original culture tube? O 2.45x108 cells/ml 9.64x108 cells/ml 3.83x10° cells/ml O 6.17x10° cells/ml O 2.24x109 cells/ml O 4.12x1010 cells/ml 5.82x10° cells/ml O 2.29x10° cells/ml O Cannot be determined 5.32x108 cells/ml
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