You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells in a volume of 200 μL. Explain how you would do this.

Q: Here is an image of a UV-irradiation (wavelength 254nm) experiment performed on a plate of E. coli…

A: UV radiations are known to cause genetic damage and cessation of bacterial growth. UV-irradiation is…

Q: If there is a sample having 3.5x10^5 CFU/ml, and this cell count came from a sample of the original…

A: CFU stands for colony-forming unit that gives rise to a particular colony. It is generally assumed…

Q: Why is there a need for an incubator in the lab? How about incubating cultures at room temperature?…

A: The Incubator is an insulated device in which the conditions like temperature, humidity, pH, and…

Q: Describe how you would prepare a dilution series of a 1 x 107 CFU/mL culture to the 10-8 dilution…

A: Serial dilutions is a process of making step by step dilutions of a substance. It is formed by…

Q: You have spread 0.1ml of a 1x10-8 diluted bacterial culture sample on a Petridish and counted 35…

A: Given  Volume plated = 0.1ml  Total dilution = 108  Number of colony = 35

Q: The laboratory that you are working in does not have a microscope or motility medium. Now, how will…

A: The motility of a bacterium is determined by the presence and absence of flagella. The flagellum is…

Q: If 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies, calculate thenumber of cells per ml of…

A: In the given example, 0.1 ml of a 1 * 10−6 dilution plate contains 56 colonies. The number of cells…

Q: Refer tó the image below to answer this question. You have a bacterial culture with a concentration…

A: Serial dilution is the dilution of a substance in the given solution in a stepwise manner. Serial…

Q: If 100,000 normal human cells were plated in a petri dish, around how many normal cells would be…

A: Introduction: If we start with one cell, when it divides, there are 2 cells in the first generation,…

Q: If a bacterial culture is started with 50 cells, what will be the cell number after four…

A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…

Q: You prepared a 7x 10^5x dilution from your bacterial culture, plated 0.2 ml of it on a Petridish and…

A: A colony-forming unit (cfu) is a unit used in microbiology to estimate the number of viable bacteria…

Q: Dilute cells from an old culture 1:200 into 5 mL of fresh media. What volume of the old culture…

A: Introduction Dilution is the process of lowering the concentration of a solute in a solution by…

Q: Would heating the culture in a sealed, small-diameter tube that is totally immersed in a water bath…

A: Microbial culture is a process of multiplication of microbial organisms by allowing them to…

Q: When the number of colony-forming units per milliliter is calculated, you will focus your counts on…

A: Petri dish or cell culture dish is used to grow microbial cultures such as bacteria and fungi. The…

Q: In the petri dish in Figure 1, the bacteria Esherichia coli (E. coli) was grown and treated with…

A: The disk diffusion technique is used to estimate the susceptibility of the organism to a particular…

Q: a. After conducting the Gram staining procedures and you observed that all cells are stained with…

A: The microorganisms that can be found in several areas of the habitat and come under the category of…

Q: You count 4 squares of a hemacytrometer and ontain 82,65,85 cells in each squy.what is the cell…

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Q: Both stained and live organisms are viewed using a microscope, but what is the purpose/benefit of…

A: Cell staining is a technique that is used in microscopy where certain chemicals can stick to…

Q: Choose the one answer that fits best. Which of the following statements regarding loading the well…

A: Electrophoresis is the process by which electrically charged particles are observed to be moving in…

Q: Which one of the following is NOT considered a biohazard?            old bacterial cultures slides…

A: Biohazard is defined as any biological substance that can pose or cause a threat and harm to biotic…

Q: The bacterium Dontuplodemeus tocheggus has a generation time of 20 minutes. Starting with one cell…

A: Bacteria are a family of rod-shaped bacteria that are often found in soil or water. They are known…

Q: In a lab experiment, there was yeast added to four different test tubes.  One tube has water, one…

A: Answer  Yeast belongs to fungus group  The four different test tubes will show different results…

Q: The image is showing Bacillus subtillis bacteria under 400x magnification, the same magnification…

A: Bacteria belongs to Kingdom Monera and are prokaryotic microorganisms that are generally…

Q: You use a spectrophometer and estimate the concentration of bacteria in a urine sample to be 4.6 x…

A: dilution factor  =10^-6

Q: You are incubating cells for 3 hours. The initial number of cells present in the sample were 102.…

A: Generation time is the time interval required for cells or a population to divide. Generation time…

Q: A student has a compound microscope equipped with 10X ocular lenses and 4X, 10X, 40X and 100X…

A: Introduction  Magnification refers to enlarging something's apparent size rather than its true size.…

Q: a. What can you observe in viewing your stained bacterial slide under the microscope if you fixed a…

A: The staining is a very important process in microbiology that is used to identify specific…

Q: When using a light microscope, objective lenses can be found to have a magnification of all of the…

A: Light microscopes are devices that are used to magnify small objects by using visible light.…

Q: Syneresis is a process by which there is the loss of water trapped within the gel matrix through the…

A: There are several biological conditions, like when serum drains from a blood clot, which is…

Q: What should be considered when preparing a monolayer cell culture?

A: Monolayer culture, also known as adherent culture, is a form of animal cell culture in which cells…

Q: If a cell sample is placed in pure water, what is the concentration of the salt inside the cell?…

A: The water move inside the cell and outside the cell through the process of osmosis. If a cell is put…

Q: In the preparation of a pure culture media, the petri plates are inverted when placed in an…

A: In microbiology, culture media is considered the growth medium that possesses the specific mixtures…

Q: Which of the following statements is TRUE about the band of molecules labeled X ( close to where it…

A: Agarose gel electrophoresis is carried out to separate DNA fragments under the influence of an…

Q: In cell counting, why do you need to calculate for the volume of media needed after computing the…

A: The cell enumeration is important for seeding the appropriate cell density into the media flask.

Q: You use the Gram staining procedure to stain a bacterium that lacks a cell wall. Would you consider…

A: The stirring process is very much important for visualisation and identification of any living…

Q: When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies…

A: Dilution is the process of decreasing the concentration of the solution. The cells count decreases…

Q: Assuming you had a bacterial culture with a concentratoion of 2x10^9 bacteria/mL, whaat volume would…

A: A microbiological culture, also known as a microbial culture, is a technique for growing…

Q: If you started with a bacterial concentration of 2,000,000 bacteria/ml and wanted to plate out 40…

A: In the question it is asked that they want to plate out 40 bacteria, and this count comes under the…

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A: Microscopy is a technique that is used to visualize objects that are not visible to the naked eyes.…

Q: You want to determine the amount of cells in a culture.  You dilute the suspension to 10^-4 and…

A: For calculating the number of bacteria or microorganisms within a sample we usually used the serial…

Q: Considering you can’t identify bacteria from a Gram stain, why might a physician perform a Gram…

A: Gram stain is a useful stain for identifying and classifying bacteria. It is a differential stain…

Q: When the number of colony-forming units per milliliter is calculated, you will focus your counts on…

A: A Petri dish (also known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish…

Q: On agar plate does each discrete colony represent the growth of one cell? Explain your answer. Why…

A: Question - On agar plate does each discrete colony represent the growth of one cell? Explain your…

Q: Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol.…

A: Gram positive bacteria has thick peptidoglycan layer and thin outer membrane. Gram negative bacteria…

Q: On the graph that relates absorbance to flavin concentration, draw a horizontal line that starts at…

A: Absorbance can be defined as the optical density that means the amount or the quantity of light…

Q: Would a syringe filter with a pore size of 0.22 micrometers be able to completely remove all…

A: Filtration refers to the sterilization technique that eliminates the microorganism. The fluid that…

Q: A 2x10-6 dilution of your bacterial culture yielded 10 colonies. What was the cell density (in…

A: The purpose of dilution of the bacterial culture before plating is to ensure that the cells are…

Q: In a serial dilution, you plate 1 ml of a 1/1000 dilution of a bacterial culture and find 25…

A: Serial dilution of the samples is used to calculate the number of microorganisms in a sample.…

Q: If you plated 0.1mL of a 103 dilution and you obtained 235 colonies on your agar plate, how many…

A: Introduction: CFU's: Colony-forming units are used to estimate the concentration of microorganisms…

Q: Dilute cells from an old culture 1:50 into 200 mL cultures. What volume of the old culture would you…

A: Culture consists of appropriate substances which provides the growth to the desired organisms.

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.