You need to calculate the volume of the forward and reverse primers you need to add to each reaction tube using the following information: The stock concentration of each primer is 10 mM. You need a final concentration of 0.3 µM in a 25 µl reaction.

Biomedical Instrumentation Systems
1st Edition
ISBN:9781133478294
Author:Chatterjee
Publisher:Chatterjee
Chapter5: Biomedical Electronics: Digital
Section: Chapter Questions
Problem 3P
icon
Related questions
icon
Concept explainers
Question

You need to calculate the volume of the forward and reverse primers you need to add to each reaction tube using the following information: The stock concentration of each primer is 10 mM. You need a final concentration of 0.3 µM in a 25 µl reaction.

Expert Solution
steps

Step by step

Solved in 2 steps

Blurred answer
Knowledge Booster
Proteins
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biochemistry and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biomedical Instrumentation Systems
Biomedical Instrumentation Systems
Chemistry
ISBN:
9781133478294
Author:
Chatterjee
Publisher:
Cengage
Curren'S Math For Meds: Dosages & Sol
Curren'S Math For Meds: Dosages & Sol
Nursing
ISBN:
9781305143531
Author:
CURREN
Publisher:
Cengage