You obtained the following raw data when setting up a Biuret standard curve: BSA (mg/ml) 0 1 2 3 4 5 6 7 8 9 10 Absorbancy 540nm 0.158 0.210 0.260 0.305 0.360 0.410 0.455 0.510 0.530 0.550 0.554 What would the quality of the line-fit (R2 value) be if you exclude experimental outliers? (Give you answer to 4 decimal places)
Q: The master formula for preparation of a ganciclovir solution is: Ganciclovir 500 mg injection vial 1…
A: Each vial contains 500 mg of ganciclovir. After 10 mL additions each of the vials gives 50 mg.
Q: Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus niger at…
A: According to Michaelis-Menten Kinetics, when the rate or velocity of an enzyme catalyzed reaction…
Q: Which of the following is incorrect? O a. Metabolic pathways involve mostly intermediates or…
A: Metabolic pathways are very important for a cell to provide energy.metabolic pathways might be…
Q: 6. Below are two bar graphs. One represents the ratio of saturated to unsaturated fatty acids…
A: Saturated fatty acids are the fatty acids without double bonds in the hydrocarbon chain. Unsaturated…
Q: Q38 (1.5): In the diagram, what does the plane draw behind the peptide bond indicate? Ca N H H R
A: A polypeptide is a long chain of amino acids linked via peptide bonds. A peptide bond is the bond…
Q: The class of the enzyme catalyzing the reaction shown in this figure is a(n) Choose the one best…
A: Given, that the enzyme can catalyse the conversion of glucose to glucose-6-phosphate by transferring…
Q: Which of the following is incorrect about the chymotrypsin mechanism? a. The (-) charge of oxygen in…
A: The proteolytic activity on the food that enters the intestine is carried out by the protease…
Q: ◆ Arginine Phenylalanine ◆ Cysteine ◆ Serine Glutamic acid ◆ Glutamine ◆ Isoleucine Leucine A.…
A: There are 20 naturally occurring amino acids. In a polypeptide chain, the alpha amino and the alpha…
Q: Table 1 shows the kinetic data that have been obtained for glucoamylase from Aspergillus niger at…
A: Enzymes catalyse the biochemical reactions by decreasing the activation energy. Enzyme kinetics are…
Q: Describe the levels of structure of a protein and tell how they would be affected by: a. Hydrolysis…
A: Proteins are composed of amino acids. They are linked together by peptide linkages. Proteins have…
Q: Two pathways that yield NADPH in the cytosol, needed for fatty acid biosynthesis, are: (1) (2)
A: Since there are multiple questions and which question is to be solved has not been specified, as per…
Q: All of the following are mechanisms for regulating enzyme activity in the cell, except: a. Rate of…
A: Enzymes are biological catalysts that drive cellular reaction and are usually protein on nature.…
Q: Which of the following is incorrect about the conversion of pyruvate (with 3 C) and CO₂ to…
A: In gluconeogenesis, glucose is synthesized from non-carbohydrate sources e.g., amino acids,…
Q: 4. Histidine, one of the 20 amino acids, contains an imidazole functional group with a pKa of 6.0.…
A: Dissociation of a weak acid is mathematically described by the Henderson-Hasselbalch equation: pH =…
Q: how can we clasify table sugar is it organic or inorganic compound?
A: Carbohydrates or carbs are macronutrient consisting of Carbon, hydrogen and oxygen atoms. In nature…
Q: With thousands of different spots on the gel, how are the identities of the proteins ascertained?
A: The protein of protein purification involves the separation of the protein and non-protein parts of…
Q: Which of the following amino acid residues cannot act as a nucleophile? a. Serine b. Glutamate C.…
A: Amino acids are the building blocks of proteins. They have side chain R groups that are important…
Q: C. 2 mM
A: Option C is the correct answer. By using the Michaelis-Menten Equation, we get the answer
Q: In an experiment, the extracellular [Na+] surrounding a nerve cell was reduced from 145 to 45 mM.…
A: In the presence and absence of an impulse, the nerve cells exist in two states known as action…
Q: You take the PKA catalytic subunit, the regulatory subunit, and the dimerization domains and mix…
A: As given in the question; the regulatory, catalytic and dimerisation domains of PKA were taken in…
Q: In fatty acid oxidation, each cycle shortens the fatty acid being catabolised by 2 carbons,…
A: Beta oxidation is the process by which fatty acids are partially oxidized and broken down into a 2…
Q: Which of the following are examples of diffusion (either passive or facilitated)? Glucose in the…
A: The process of diffusion is a passive, spontaneous process and occurs down the concentration…
Q: the following peptide is incubated as chymotrypsin: Gly-Val-Phe-Lys-Ala. Present the detailed…
A: Peptides are small amino acid sequences.The proteolytic enzymes like trypsin, pepsin,…
Q: Describe the composition of bacterial cell walls.
A: The cell wall in bacteria tends to form a stable frame of constant thickness all around cell and is…
Q: true or false 1. Phosphoenolpyruvate carboxykinase is the enzyme involved in the carbon dioxide…
A: Photosynthesis and photorespiration due to the fact that photosynthesis occurs when the RuBisCO…
Q: Why is there a need for human experimentation?
A: Human Experimentation means the physician’s departure from the standard medical practice of…
Q: discuss the purpose of the hexose monphosphate shunt, and explain the relationship to this pathway…
A: The hexose monophosphate shunt (HMP) is a mechanism that doesn't produce any energy in the form of…
Q: Which of the following is incorrect about the triacylglycerols? a. If they are of animal origin,…
A: Triacylglycerols, commonly known as triglycerides, are the most widely recognized type of lipid.…
Q: which intermediate of the citric acid cycle regulates the flow of metabolites through glycolysis,…
A: The citric acid cycle also known as the Krebs cycle or the TCA cycle is a series of chemical…
Q: In the G protein signaling pathway, the 2nd messenger is: a. ATP b. GDP Oc. Cyclic GMP d. Cyclic AMP…
A: The G-protein signalling pathway play important role in metabolic regulations, embryonic…
Q: Which of the following is a property of both D-altro O They are found in sucrose. O They are major…
A: Monosacharides are the monomers of Polysaccharides that are joined by Glycosidic bonds .…
Q: 1. a) What is meant by the term "buffer solution"?
A: A buffer solution is an aqueous solution that can be used to maintain physiological pH when a small…
Q: Estimate the initial rate, vi, at each initial substrate concentration assuming that only the data…
A: Time(minutes) So = 10mM So = 15mM So= 20mM So =40mM 0 0 0 0 0 1 1.03 1.08 1.12 1.15 2 2.10…
Q: Epinephrine B-Adrenergic receptor α В Figure 13.04 Biochemistry, Third Edition 2015 W.H. Freeman and…
A: As there are two images and it's not mentioned which question needs to be answered, therefore I am…
Q: Which are the energy investment steps in glycolysis that transfer phosphate from ATP to a substrate
A: Glycolysis is the process of breakdown of glucose to produce energy in which glucose, a six-carbon…
Q: [S] (MM) 0.5 1.0 1.5 Vo No Inhibitor (mM min-¹) Vo With Inhibitor (mM min¹¹) 16.67 25.25 30.49 23.5…
A: Enzymes are protein molecules that increase the rate of reactions by decreasing the activation…
Q: Technique Initial IMAC IEX Gel Filtration Protein (mg) 3467.8 196.7 153.6 142.1 Enzyme activity…
A: Technique Protein Enzyme activity Overall yield Specific activity Overall Enrichment initial…
Q: How biotechnology evolved from ancient biotechnology, classical biotechnology, and modern…
A: Introduction: Biotechnology is technology that utilizes biological systems, living organisms or…
Q: Which of the following is incorrect? a. Protease inhibitors are small proteins that pose as protease…
A: Proteases are enzymes that can cause the hydrolysis of peptide bonds and can thus cleave the protein…
Q: The graph shows the number of microbial cells (log scale) against time in a liquid culture. In which…
A: Bacterial growth is divided into four stages. During the lag phase, the bacterium prepares itself by…
Q: 4. Exhibit 4A The following question(s) refer to this peptide: Cys-Ala-Gly-Arg-Gln-Met Refer to…
A: The charge of the peptide is due to the ionizable groups present in the amino acids. The charge on…
Q: Mention four advantages and two limitations of capillary electrophoresis over HPLC.
A: Capillary Electrophoresis (CE) is a widely used separation technique in biochemistry. Here a very…
Q: Human Response to Cold Temperatures 2. Hypothermia: In hypothermia, the body temperature is lowered…
A: The human have multiple physiological responses to combat the cold weather so that maintain the…
Q: Which of the following is incorrect? a. The ratio "catalyzed rate/uncatalyzed rate" is known as…
A: The Enzymes as biological catalysts. In the enzyme catalyzed reaction, the substrate is converted to…
Q: Glycophorin is an integral membrane protein found on the surface of red blood cells that is…
A: Glycophorin is a single pass total alpha-helical integral membrane protein. This protein plays an…
Q: An enzyme has a maximal Velocity of 110.36 uM/s. At a substrate concentrationof 8.5 uM/s. What is…
A:
Q: Which of the following is incorrect about the drugs called statins? O a. None; all the other choices…
A: The regulation of cholesterol synthesis can be explained simply in terms of economics. SREBP is…
Q: List ALL of the substrate-level phosphorylation steps in cellular respiration and the enzymes that…
A: Substrate level phosphorylation is the metabolic process by which nucleotide tri phosphate ATP or…
Q: Lipids are common components of membranes and cell walls. What is the structural motif of membranes?…
A: Lipids are biomolecules that do not have a fixed chemical structure like carbohydrates or amino…
Q: The enzyme Lysozyme is present in human nasal mucus and catalyzes the breakdown of a component of…
A: Hi! Thank you for the question. We are authorized to answer three subparts at a time, since you have…
Step by step
Solved in 2 steps with 2 images
- QUESTION 1 What is 105 ng ml in: a. ug/ml b. mg 1 C. ug1 d. g/ul (Please list each answer on a separate line, beginning with the corresponding letter. Don't forget units for each answer!) For the toolbar, press ALT+F10 (PC) or ALT-FN+F10 (Mac). 14px E E YA BIUS Paragraph Arial 田 Bヨ田田用 国 田EEt oQuestion 17 (3 points) An order is for Compazine 8mg IM q4h prn. Available is Companize 10mL multidose vial at 5mg/mL. How many milliliters will you give and what size syringe will you use? Question 18 (3 points) A doctor orders streptomycin 500mg IM q12h. The vial you have contains streptomycin sulfate for injection with 1 g/2.5mL of solution. How many milliliters will you give and what shize syringe will you use? Question 19 (3 points) nighs 55 nounds. Paily dosQUESTION 3 You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 0.420 3 0.510 4 0.610 0.720 0.810 7 0.915 8 0.950 9 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you exclude experimental outliers? (Give you answer to 4 decimal places)
- Question 33 Orciprenaline (C₁₁H₁7NO3, 211.258 g/mol) is a bronchodilator used in the treatment of asthma. The BP assay for orciprenaline tablets is described below. HO The following information was obtained during the assay: Weight of 20 tablets = 2.5564 g Weight of tablet powder assayed = 0.5266 g Absorbance reading - 0.5868 Stated content per tablet = 20 mg A (1%, 1 cm) at 276 nm = 72.3 OH H N OH "Weigh and powder 20 tablets. Shake a quantity of the powder containing 80 mg of orciprenaline sulphate with 50 mL of 0.01 M hydrochloric acid (HCI), filter and add sufficient 0.01 M HCI to the filtrate to produce 100 mL. Dilute 10 mL to 100 mL with 0.01 M HCI and measure the absorbance of the resulting solution at the maximum at 276 nm." Orciprenaline 4 Answer the following questions, explaining your reasoning clearly. a) Calculate the amount of orciprenaline in the tablet powder analyzed. b) Calculate the average amount of orciprenaline per tablet. c) Calculate the percentage of the stated…QUESTION 4 You obtained the following raw data when setting up a Bradford standard curve: BSA (mg/ml) Absorbancy 595nm 0.225 1 0.310 0.420 0.510 4 0.610 0.720 6 0.810 7 0.915 8 0.950 9. 0.980 10 0.990 Set up a standard curve. What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) N MQUESTION 2 You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0.158 1 0.210 2 0.260 0.305 4 0.360 0.410 0.455 7 0.510 8 0.530 9. 0.550 10 0.554 After blanking against a biuret-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.324 was obtained. curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / Set up a standard ml (up to 3 significant figures). 5
- QUESTION 2 You obtained the following raw data when setting up a Bradford standard curve: Absorbancy BSA (mg/ml) 595nm 0.225 0.310 2 0.420 0.510 4 0.610 0.720 0.810 0.915 0.950 9 0.980 10 0.990 After blanking against a bradford-dH20 sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.583 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg / ml (up to 3 significant figures).QUESTION 2 You obtained the following raw data when setting up a Bradford standard curve: 0 1 2 3 4 BSA (mg/ml) 595nm Absorbancy 6 7 8 9 10 0.225 0.310 0.420 0.510 0.610 0.720 0.810 0.915 0.950 0.980 0.990 After blanking against a bradford-dH₂O sample, the protein concentration of an unknown sample was determined using the same method and an absorbancy of 0.568 was obtained. Set up a standard curve, excluding outliers (experimental and statistical) and determine the protein concentration in the unknown sample in mg/ml (up to 3 significant figures).Question 2 You are checking a calculation that a foundation pharmacy student has performed. The question asks that 120 suppositories weighing 1 gram are to be made, containing 300mg Zinc Oxide. The displacement value of ZnO is 4.7 in the base that is to be used. How many grams of base would be required? units - g Answer to 2 decimal places Amount of base required = theoretical amount – displaced amount Where; Displaced amount = (amount of drug in g/DV of drug).
- Question 2 of 20 0.1 mg The dosage strength of Granisetron Hydrochloride is written on the drug label as v. i mg per mL. Complete the fractional ratio that expresses this dosage strength (known ratio): ? mL NDC 25021-778-01 Ronly 1024061 Granisetron Hydrochloride Injection, USP SAGENT" Mfd. for SAGENT Pharmaceuticals Schaumburg, IL 60195 (USA) Made in India ©2013 Sagent Pharmaceuticals, Inc. Code No.: TN/DRUGS/TNO0003234 0.1 mg per mL Lot: For Intravenous Use Only 1 mL Single-Use Vial Exp.: Enter the correct numeral of mL. Do not include units in your answer. Enter your answer. 8 mL Previous Next Sterile, Nonpyrogenic, Preservative-free. Store at 20° to 25°C (68° to 77°F). Do not freeze. Protect from light. Single Use Only. Discard Unused Portion. (01)00325021778012Question 1 Ordered Demerol 200mg and Vistaril 250mg IM. You have Demerol in 2 ml ampul with 100 mg/ml. Vistaril is in a 20 ml multi - doses vial labeled 150 mg/1.5ml. Wha is the total volume to be given? (If rounding is required, round to the nearest tenth).Question 12 (5.333 points) Listen A 1-year-old child weighing 26 lbs is prescribed ibuprofen 60 mg orally every 6 hours. The nurse has ibuprofen suspension 100 mg/5 mL on hand. The safe dose range is 5-10 mg/kg every 6-8 hours for a maximum of 40 mg/kg/day Blank 1: Calculate the single safe dose range in mg/dose: (round to nearest tenth) Blank 2: How many mL would this dose be to administer? (round to nearest tenth) Blank 3: Should the nurse administer this dose (yes/no, if no, why?) Blank # 1 Blank # 2 Blank #3 Question 13 (4.444 points) Listen A/ A 38 lb child is prescribed phenobarbital 10 mg orally every 12 hours. The nurse has phenobarbital 15 mg/5 mL in the med cart. The SDR is 1 to 6 mg /kg daily in two divided doses not to exceed 100 mg/day.