You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence, resulting in a non functional protein product. You remove blood stem cells from a patient and use a virus to insert which of the following sequences into the stem cells (select all sequences you need). ORNAi for the mutated gene Cas9 gene Donor DNA with wild type DNA sequence Guide RNA that matches the mutated gene Donor DNA with mutant DNA
You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a missense mutation that changes a glutamic acid to a valine in the beta-globin coding sequence, resulting in a non functional protein product. You remove blood stem cells from a patient and use a virus to insert which of the following sequences into the stem cells (select all sequences you need). ORNAi for the mutated gene Cas9 gene Donor DNA with wild type DNA sequence Guide RNA that matches the mutated gene Donor DNA with mutant DNA
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
Related questions
Question
Please help a bit confused
Transcribed Image Text:You would like to design a method to treat the most common mutation that causes sickle cell anemia using CRISPR/Cas. This mutation is a missense mutation that
changes a glutamic acid to a valine in the beta-globin coding sequence, resulting in a non functional protein product. You remove blood stem cells from a patient
and use a virus to insert which of the following sequences into the stem cells (select all sequences you need).
RNAi for the mutated gene
Cas9 gene
Donor DNA with wild type DNA sequence
Guide RNA that matches the mutated gene
Donor DNA with mutant DNA
Expert Solution
This question has been solved!
Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.
Step by step
Solved in 2 steps
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education