Gram staining

specific organism. For example, some gram negative bacteria can produce endotoxins when they die, which would be of concern to a doctor prescribing antibiotics. Identifying a specific bacteria as a source of a disease is the first step in moving towards discovering how it can be transmitted and preventing reoccurrence. Species can be distinguished by morphology and

A gram stain is a simple way to begin differentiating an unknown bacterial sample. Bacteria may either be gram-negative or gram-positive. Staining makes bacteria cells visible, and this particular staining method allows us to narrow down whether the bacteria in gram-negative or gram-positive. Gram-negative stains purple and gram-positive stains pink. Gram staining is used by students in advanced placement biology high school courses and in college microbiology courses. Gram staining is conducted

study was to identify the There are a group of tests used specifically to differentiate bacteria in the Enterobacteriaceae family. These Results Table 1 Microscopic Data of Differential Gram Stain Gram Stain (A) Gram-negative Cell Results (B) Gram-positive Cell Results Before Staining Transparent Color Transparent Color After Crystal Violet Stain Purple Color Purple Color After Iodine Stain Purple Color Purple Color After Decolorization with Alcohol Transparent Color Purple Color After

it is gram negative or positive) the specimen would have to undergo differential (Gram) staining. Hans Christian Gram was the Danish bacteriologist behind the gram staining technique. His Gram stain technique led to the discovery that Typhus bacillus did not retain the stain. Another procedure like differential staining is called simple staining. The main difference with this procedure is its simplicity and use of just one dye while the differential staining is more complex, uses more staining and

Most bacteria are classified as either gram positive or gram negative. Gram positive is a purple stain and gram negative will appear pink or red. Gram negative can cause many kinds of infections. One type of bacteria is Escherichia which is a rod shaped bacteria. A Gram stain is used in lab setting to identify what type of bacteria is present. A sample is placed in a nutrient media and incubated. This media will encourage bacteria growth present. This process allows for further testing and to identify

discusses the technique of Gram Staining in order to characterize bacteria. Gram-staining is a process in which the cells are immersed in crystal violet, iodine, ethanol, and safranin. Based on bacteria’s cell wall, most common bacteria are either Gram-positive or Gram-negative. The Gram-positive cell wall are composed of multiple layers of peptidoglycan layers, whereas the Gram-negative cell wall has one layer of peptidoglycan. Through the technique of Gram-staining, the Gram-positive cells will turn

potential to be round (cocci), rod (bacilli), or spiral shaped. Gram-staining is a technique that can help the scientist learn more about the bacteria. Gram-staining was a technique developed by H.C. Gram in 1884. Gram-staining is intended to categorize bacteria by their composition regarding cell wall composition, cell wall layering, bacteria size, and more.

another technique known gram staining. Gram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents4. This technique helps to identify among Gram positive and Gram negative groups by coloring these cells pink or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with. Alternatively, Gram negative bacteria stain

to satisfactorily grow, collect data on and successfully identify an unknown given culture. Materials and Methods: 1. Gram Staining: In order to determine the type of bacteria on hand by using the dichotomous key within the laboratory manual the first test to be utilized would consider whether the microbe presented is gram positive or gram negative. In order to perform a gram stain you must utilize the steps listed in figure 7.1 of the laboratory manual as well as the four basic steps. These steps

Gram staining is a way of classifying bacteria into two groups: gram-positive and gram-negative. The experiment conducted allowed for distinguishing of two specimens, Bacillus subtilis and Escherichia coli, using several dyes to distinguish the two. The experiment revealed the culture of B. subtilis to be a Gram-Positive organism and E. coli to be a Gram-Negative organism. Introduction Cell staining is a method that can be used to view cells and cell components with a microscope. The structure