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Ap Biology

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AP ESSAY ANSWERS: 16-20
1. Information transfer is fundamental to all living organisms. For TWO of the following examples, explain in detail, how the transfer of information is accomplished. A) The genetic material in one eukaryotic cell is copied and distributed to two identical daughter cells. B) A gene in a eukaryotic cell is transcribed and translated to produce a protein. C) The genetic material from one bacterial cell enters another via transformation, transduction or conjugation. 16 total pts possible
A) max 8 pts combined (4 pts max each part – Part a is looking for “copy and distribute”) “copy”= DNA replication -when DNA is copied- interphase, S phase of cell cycle -recognition of origin site on DNA …show more content…

The human genome illustrates both continuity and change.
A) Describe the essential features of TWO of the procedures/techniques below. For each of the procedures/techniques you describe, explain how its application contributes to understanding genetics. -The use of a bacterial plasmid to clone and sequence a human gene -Polymerase Chain Reaction (PCR) -Restriction Fragment Length Polymorphism (RFLP) analysis
B) All humans are nearly identical genetically in coding sequences and have many proteins that are identical in structure and function. Nevertheless, each human has a unique DNA fingerprint. Explain this apparent contradiction.
16 points possible
A) max 12 pts total (6 pts for each part of the subquestions) Describe= max 3 pts, 1 for each bullet Explain= max 3 pts, 1 for each bullet Describe plasmid cloning: -cut plasmid with restriction enzyme -cut/isolate human sequence with the corresponding restriction enzyme -mix/ anneal/ligate -introduce recombinant plasmid into bacteria -select recombinant bacteria (ex. antibiotic resistance, fluorescence, reporter gene, etc) -bacteria reproduction used to amplify the sequence (PCR) -describe either degradative (Maxam-Gilbert) or dideoxy (Sanger) method

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