En una extensa revisión de la literatura los científicos desarrolladores de la base de datos lograron recolectar 288 proteínas que han sido reportadas bajo la clasificación de "moonlight proteins"; con las cuales desarrollaron la base de datos conocida como Multitasking Proteins DataBase o Moonlighting Proteins DataBase. Esta base de datos se puede acceder por medio de la dirección http://wallace.uab.es/multitask/ (Hernández, 2013).
En general la base de datos provee información sobre las funciones que realiza cada una de las proteínas "moonlight" disponibles en la base de datos junto con su NCBI y UniProt "accesion number", el estado oligomerico y monomerico de la proteína, código PDB y referencias bibliográficas sobre las funciones designadas a cada proteína. Dentro de las características de la base de datos hay que mencionar que los desarrolladores nombraron como función canónica a la primera función que fue descubierta de la proteína y "moonlight" a la segunda función que haya sido descubierta de la misma proteína. Multitasking Proteins DataBase posee un formato y diseño sencillo que facilita la obtención de información sobre las proteínas moonlight disponibles en la base de datos. El grupo de trabajo que desarrollo la base utilizaron MySQL que es un sistema de gestión de bases de datos muy rápida y utilizada para la creación de aplicaciones web. Este sistema se caracteriza en que provee una colección estructurada de tablas que contiene la información que se desea
This must be in your own words and not copied and pasted from the original source. Include the purpose of the database and the subject matter it covers. This may be four or five sentences; and
Wikimedia Foundation, I. (2014, 10 13). From Wikipedia, the free encyclopedia. Retrieved from From Wikipedia, the free encyclopedia: http://en.wikipedia.org/wiki/MySQL
A protein has multiple existing structures, these are the primary, secondary, tertiary and quaternary structures which occur progressively. A protein is essentially a sequence of amino acids which are bonded adjacently, and interact with one another in various ways depending on the R group that the amino acid contains. There are 20 different amino acids which are able to be arranged in any given order, thus giving rise to a potential 2.433x1018 (4.s.f) different combinations, and therefore interactions between the various amino acids.
Proteins are primarily considered to have one primary function to serve its role in an organism, however studies have observed to have multiple functioning proteins known as moonlighting proteins (Khan et al. 2014). Moonlighting proteins along with primary functions, have secondary functions that are not related to the primary function and does not correlate to the primary or other functions (Khan et al. 2014). The multifunctional proteins play essential roles in carrying out biochemical functions which aids in the cell growth but are not caused by gene fusion and multiple RNA splice variants (Amblee et al. 2015). The discovery of moonlighting proteins was first discovered by Piatigorsky and Wistow while observing crystallins (Khan et al. 2014). Crystallins, are structural proteins that are found in the eye lens that exhibit enzymatic activity to make the lens itself (Khan et al. 2014). Crystallin has a primary function to help form the lens of the eye by acting as a structural protein (Amblee et al. 2015). Besides enzymatic activity, crystallin was observed in other mammals to have secondary functions such as metabolic functions which are helpful in prokaryotic (Khan et al 2014). Most moonlighting proteins are characterized as cytosolic enzymes and chaperons, or in other words helping proteins (Amblee et al 2015). The multifunctional proteins or moonlighting proteins can also be identified as receptors, channel proteins and ribosomal proteins (Khan et al. 2014). Due to the
The basic building blocks of proteins are amino acids, the biuret reaction tests for protein. A solution of sodium hydroxide is added to a sample then a few drops of copper sulphate solution, if positive – the solution will turn mauve. There are 20 different amino acids and they can be joined in any order. Therefore there can be many different functions. A protein consists of one or more polypeptide chains (a polypeptide chain being multiple amino acids joined together via condensation, producing a peptide bond). Different proteins have different shapes as the shapes are determined by the sequence of amino acids.
There are several important steps to consider when designing a database, as a well-designed database should be deployed and not only support the accuracy and integrity of business information but also avoid redundant data and assist with has enterprise level reporting tasked. If we analyze the
Sophisticated software compared these parts using existing proteins of the human genome to determine the actual proteins in the samples. They found that the Maiden's profile of
The concept of protein domains and motifs has dominated the first half of this course. Discuss the relevance of protein domains to the following topics:
They are known to be the workhorses of the body cell that carries out diverse catalytic and structural roles into building the structures of all living organisms [15]. The basic structure of protein is a chain of amino acids that supplies energy to a body. There are 20 different naturally occurring amino acids that make all types of protein. Proteins come in various sizes and shapes. Some comes in a thread-like shape known as fibrous proteins and they tend to have structural or mechanical roles. Others come in spherical shapes, known as the globular proteins [16]. These spherical proteins function as enzymes, transport proteins, or antibodies. The key function of protein is based on its ability to recognize and bind specific ally to molecules, it also need to be in the right shape in other to function properly [15]. The primary structure of proteins is a linear sequence of amino acids encoded by DNA. This sequence controls how protein folds into three dimensional structure, the stability of its resulting structure [17], and functions. It is important to add that protein is an important building block of bones, skin, blood and
At the end of this unit, students will be able to use the terms DNA, RNA, protein, and nucleotide when it comes to protein synthesis. They will be able to explain how transcription and translation are processes of protein synthesis. They will be able to use genetic code table to translate an RNA sequence into an amino acid sequence. Students will be able to demonstrate their understanding of the Central Dogma. They will be able to describe the semi-conservative nature of DNA replication. They will be able to explain how a change in the DNA sequence code can alter protein function.
Methods: Firstly in this experiment, one had to get familiar with how to use many of the basic commands in FirstGlance in Jmol. To get familiar with the program, one did a tutorial analysis of protein PDB ID: 1LGD at http://bioinformatics.org/firstglance/fgij//, following the specific step listed in the handout.1
With modern advances to genome sequencing, whole genomes can now be used to rapidly identify protein encoding sequences from a small amount of amino acid sequences. This has led to a field of study called proteomics, which encompasses the study of proteins, protein complexes, and protein-protein interactions. As an example when one isolated protein is found to interact with a complex of proteins known to be part of an enzyme complex, it could be inferred that the isolated protein may be part of the enzyme complex as well. Some of the techniques involved for studying proteomics are interaction assays that allow for the rapid identification and purification of proteins.
In most instances, protein molecules are usually embedded from hundreds to thousands of amino acids. A repertoire of twenty different amino acids, joined in any possible sequence allows the existence of an inconceivably large number of proteins that is infinite in nature.
Information is the backbone to today’s society and it is constantly being shared and analyzed by everyone from students to professionals to anyone that has an interest in the subject. Data can be made available to the general public or to a subset of individuals who pay for it. Especially with data that is shared freely can be just a text/csv file of information without any real database schema defined like the one used in this experiment. This information can be manipulated, the structure changed and indexes can be created to aid in what queries that individual is running.
Different techniques and principles for protein extraction and characterization were demonstrated in this experiment. Various proteins were extracted from different sources: 1.67 g yeast invertase, 1.03 g egg white albumin, and 5.15 g of milk casein. Activity assay for invertase was performed using Benedict’s test and the enzymes inverting action on sucrose was confirmed. Warburg-Christian Method and Bradford Assay were also employed to determine the protein concentration in the albumin and the casein samples. The concentrations for the albumin and casein samples were found to be 0.519 and 0.327 mg/mL, respectively based on Warburg-Christian Assay; and 6.5x10-3¬ and 1.9x10-2 mg/mL