1. Compare and contrast DAT from IAT based on procedure and clinical significance. 2. Explain the importance of the three phases of Indirect Coomb's test. 3. Differentiate the three potentiators used in Coomb's Test (PEG, LISS, 22% BSA)
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1. Compare and contrast DAT from IAT based on procedure and clinical significance.
2. Explain the importance of the three phases of Indirect Coomb's test.
3. Differentiate the three potentiators used in Coomb's Test (PEG, LISS, 22% BSA)
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- 2. Provide information about the TLI test.1. Why is a 1:20 dilution of patient serum, rather than undiluted patient serum, used for the qualitative test? 2. What level of Rheumatoid Factor in serum is clinically significant? 3. Describe how the Rheumatoid Factor concentration is computed.1. Differentiate the 2 specific types of VDRL namely: 1.1 quantitative VDRL 1.2 qualitative VDRL 2. Describe the principle behind the RPR (Rapid Plasma Reagin) Test. 3. What precautionary measures should be observed in the collection and preparation of specimen for VDRL examination?
- 1. define the latex agglutination (LA) and gelatin particle agglutination tests (GPAT) techniques 2. State the principle underlying the above techniques 3. state the types or forms of the techniques if present1. How will you differentiate VDRL from RPR Card test? 2. Give the composition of VDRL reagent and their function.2. Discuss the different types of antibiotic based chemical composition. Give examples for each 3. Enumerate 3 qualities of a good chemotherapeutic agent. 4. What are the different methods of sensitivity testing? Discuss briefly.
- 3. Discuss the applications of the direct and indirect antiglobulin tests.Draw the steps of Qualitative Test in LATEX AGGLUTINATION TEST FOR THE DETECTION OF RHEUMATOID FACTOR 1. Bring the test reagents and samples to room temperature (Note 1). 2. Shake the latex vial gently. Aspirate dropper several times to obtain a thorough mixing. 3. Place 1 drop (50 µL) of the serum under test into one of the circles on the card. Dispense 1 drop of positive control serum and 1 drop of negative control into two additional circles. 4. Add 1 drop of ASLO-Latex Antigen to each circle next to the sample to be tested. 5. Mix the contents of each circle with a disposable stirrer while spreading over the entire area enclosed by the ring. Use separate stirrers for each mixture. 6. Rotate the slide by means of a mechanical rotator (100 r.p.m.) for a period of 2 minutes (Note 2). 7. Observe immediately under a suitable light source for any degree of agglutination.i. The 21 Day (or 3 Weeks) Cumulative Irritancy Patch test ii. The Repeat-insult Patch Test Describe the procedure of the two tests and the interpretation of their results for the presence of allergies.
- Answer the questions briefly and concisely. Describe the limitations of FANA Describe the other Assays for ANA testing What are the advantages of FANA over the other assays? Describe the limitations of the RF Agglutination test What are the sources of errors in RF agglutination?REACTIONS OF CARBOHYDRATES Reagents:Samples:10% glucose, galactose, fructose, xylose, maltose, sucrose, lactose, starch (5 ml each) ,2 ml Fehling's A, 2 ml Fehling's B, 8 ml Benedict's reagent, 8 ml Barfoed's reagent, 3 g sodium acetate, 2 g phenylhydrazineHCl Materials: 12( 20 ml) test tubes, test tube rack, test tube brush, test tube holder, alcohol lamp, tripod, wire gauze, water bath, (10 ml) graduated cylinder, dropper, stirring rod, beaker(250 ml), spatula, microscope,7 glass slide, 7 cover slip, watch glass, platform balance Procedure: 1. Osazone formation –Mix 3 g sodium acetate and 2 g phenylhydrazineHCL and 14 ml distilled water. Warm with stirring until solution clears. Place 1 ml sugar solution (glucose, fructose, galactose, maltose, xylose, sucrose, lactose) in separate tube. Add 2 ml of the hot solution in each of the sugar solution, stopper with cotton, mix well and heat in a boiling water bath for 30 minutes. Cool at room temperature and examine the crystals under the…1. The urine dipstick immunoassay only provides a presumptive result for the presence of drugs. What is the confirmatory test for drugs? 2. What is the cut-off range for methampbetamine and tetrahydrocannabinol in the Philippines? Why was it established at that level? 3. Enumerate 5 common adulterants for urine drug screening.