1. You need to make 125ml of Polyacrylamide (solid) to make the gel at different percentages. Show a calculation if you wanted to make the following gels? 1% = 1g/100ml A. 5% B. 12%
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- CHROMATOGRAPHY HW 1. What does adsorb mean? 2. What is chromatography used for? 3. Why must a pencil rather than a pen be used to mark the TLC plate? 4.If you make an error recording data, how would you correct it? Is it ok to erase it or white it out? 5.Using the data on the last slide, calculate the Rf value for each spot.does melting point determination remove impurites in crude protein?2. Although we use pre-cast gels in this lab it is common practice to make your own polyacrylamide gels in a laboratory. How much bis-acrylamide (in grams) should you use to make 5 mL of a 5% bis-acrylamide solution? 3. We typically store running buffer (used in SDS-PAGE) in a 10X concentrated form. You are given 100 mL of a 10X running buffer stock solution and need to make it all into a 1X running buffer solution. How much water would you add to the stock solution?
- Q.2: Write the Separation of heavy metals using solvent extraction technique.2 d) In a typical HPLC column, 150 atm of pressure is required to push the mobile phase through the column. Why is high pressure needed in HPLC? e) Why are HPLC particles porous?purification of egg yolk vitelline 1) Measure the volumes of 3 egg yolks and mix by adding an equal volume of NaCl solution. 2) Extract the mixture with 3 solid volumes of ether and separate the aqueous phase. 3) Do the same procedure 3 times. 4) Mix the sample with water and rinse. 5) It is expected for the protein to collapse. 6) Some more water is added in order to check whether the collapse has occurred completely. 7) The sample is centrifuged and the precipitate is dried.For each step, explain why that action was taken. for example why is NaCl added? Why is the procedure repeated 3 times?
- Use special gel loading tips or a micro-syringe to load the complete sample in a narrow well. Take care not to poke the well bottom with the tip as this will create a distorted band. Never overfill wells. This could lead to poor data if samples spill into adjacent wells, and poorly resolved bands. Load 20-40 μg total protein per mini-gel well. -If you have a lysate with protein concentration of 1500 ug/mL, how much sample you need to load according to the step indicated in D.349. R Lactic acid Salicylic acid Trichloroacetic acid Flexible collodion qs ad Sig: wart remover. Use as directed. 48 5 g 452 to an to 2 g 100 g a. Flexible collodion contains 20% w/w camphor and 30% w/w castor oil. How many grams of each would be contained in 100 g of the mixture? b. The specific gravity of castor oil is 0.955. How many milliliters of the oil are contained in 100 g of the mixture? c. If the specific gravity of the mixture is 0.781, what are the percent w/v concentrations of lactic acid, salicylic acid, and trichloroacetic acid in the mixture?How does capillary zone electrophoresis (CZE) works.
- PART1: Put True (T) or False (F) for the following sentences: 1. chromatography" derived from two Greek words "Chroma" meaning color and "graphein" meaning to write. The stationary phase may be a solid, or a liquid supported on a solid or gel. 3. The mobile phase may be 2. Solid. 4. Silica gel is the most common stationary phase in adsorption chromatography. 5. Liquid Chromatography abbreviated as (GC ). 6. In Thin Layer Chromatography, the mobile phase is gas. 7. a = KB/KA, where Kg is the distribution constant. 8. k'B and k'A are the retention factors. 9. Rf, is the distance traveled by the compound ONLY. 10. Chromatography is a physical method of separation.A measurment for the Lambda-max for a colored solution is 671 nm with a very high Absorptivity. What does the color of the solution look like in the sunshine? and What does the color look like in a room where the only light source emits 671 nmIn a surfactant molecule the active head surface area was 7.0 (secured angstroms) whereas it is hydrocarbon chain length and volume were 20 (angstroms) and 50 (cubic angstroms) respectively. The expected micellar shape is then? 00 Select one O a. Spherical O b. Rod-like O c. lamellar Od None FINISH ATTEMPT