13. Southern blotting is separation of DNA by agarose electrophoresis and probing with a labeled primer. By analogy, Eastern blotting is: A a method that analyzes nucleotides and small RNA fragments B. a method that analyzes ribosomal RNA C. a method that analyzes transfer RNA D. a method that analyzes messenger RNA E. all of the above F none of the above

13. Southern blotting is separation of DNA by agarose electrophoresis and probing with a labeled primer. By analogy, Eastern blotting is: A a method that analyzes nucleotides and small RNA fragments B. a method that analyzes ribosomal RNA C. a method that analyzes transfer RNA D. a method that analyzes messenger RNA E. all of the above F none of the above

Biology (MindTap Course List)

11th Edition

ISBN:9781337392938

Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Publisher:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. Berg

Chapter15: Dna Technology And Genomics

Section: Chapter Questions

Problem 10TYU: DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c)...

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Question Image hown bele nallest? Q. A gel from gel electrophoresis is shown below. Which DNA fragment is the smallest? B. Direction of Travel
DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkages
Which of the following statements on restriction enzymes is correct? I. to cut phosphodiester bonds of foreign DNA II. to serve as a defense mechanism for bacteria III. to recognize DNA sequences with site-specific manner Lütfen birini seçin: O a. Only III O b. I, II, III О с. Only I O d. Only II O e. I and II
9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation method Description Ligated product incubated with competent E. coli, heat-shock treatment. Cells incubated with the plasmid DNA are subjected to a high-voltage pulse. Transiently renders the bacterial membrane permeable to large molecules. The process of injection of foreign DNA into a cell using micropipette/micromanipulator.
Gene cloning vectors should have the property of self-replication so that DNA insert can be copied as the vector replicates. Lütfen birini seçin: O a. True O b. False
Which of the following would be the best assay to use to determine if a protein binds to RNA? O northern blot O PCR O Southern blot O Electro Mobility Shift Assay (EMSA) O western blot
_1. Bacterial proteins that have the ability to cut both strands of the DNA molecule at certain points 2. Contain foreign DNA 3. Is made by connecting segments of DNA from different sources _ 4. General term for a carrier used to transfer a foreign DNA fragment into a host cell 5. A small ring of DŇA found in a bacterial cell 6. The procedure for cleaving DNA from an organism into small segments, and inserting the f. genetic engineering or segments into another organism a. recombinant DNA b. vector c. restriction enzymes d. plasmid e. transgenic organisms recombinant DNA technology
7. Consider the following plasmid (size 6700 bp), with restriction sites at the positions indicated: BamHI + 1 6700 bp 2800 3500 EcoRI BamHI Probe
Gene therapy can be done by? O Lamda phage O totipotent O CRISPR-Cas9 O pluripotent O dihybrid
1. Draw a gel showing the bands/fragments generated from cutting the plasmid below with Sall, BamHI and Pstl. Make sure you label the bands with the expected sizes. PstI 3607 3000 4000 amp ori HindIII EcoRI 4359 0 29 EcoRV 2295 1 NdeI 185 pBR322 4361 bp 375 tet 2000 BamHI 651 Sall 1000 2. If you want to use the plasmid above to clone an insert of 500 bp using BamHI and Sall, what would your expected FINAL plasmid+insert size to be if your cloning was successful? (remember that you first have to treat the intact pBR322 with BamHI and Sall to prepare it for introducing the insert).
3. Which of the following is not an important characteristic of a bacterial plasmid vector (shown)? (4) Plasmid pBR322 Host: E.com Ampicillin resistance- reporter gene lamp O Orgh of replication Hrdl +-Dants San Tetracycline rositance reporter gene (7) It should have the ability to integrate into the host's genome. It should have a reporter gene. It should have a gene for a selectable marker. O It should be small in size. O It should have the ability to amplify the gene being carried by producing many copies within the host cell. See Concept 13.2: DNA Can Genetically Transform Cells and Organisms
3. Using the same template strand, transcribe the DNA:GCA TAG CAA TGC