1. A two-component mixture is analyzed by paper TLC. Component A is more soluble in the mobile phase thancomponent B. The following result is obtained. Calculate the Rifor each component and label the identity of eachFrond (3-0,4)CM3D2.6cmrA (2-6-04 m = 2.2cmB C67-0.ycm=0.3cmRF (A= 2.2cm = 0.852. Lemspot. 239でSolvent FrontA0-2.6m%3D3cmSpoting Line Rf (8- 0.3cm2. Scm= 0.12%3D2. Which one is the more polar component? Explain IS morC polo Becauseits Rf Is low3. If a more polar solvent (mgbil phase) is used for the above experiment, the result will be:O Both component A and Component B will travel further up the plate-Distance may verurb) Only Component A will travel further up the platc) Only Component B will travel further up the platd) They will travel on the plate the same distance as when a less polarChoose one of the answers and explain4. Paper chromatography works as the same principle as TLC however, cellulose paper (the same as filter paper) isused for stationary phase. An unknown liquid sample is analyzed using paper chromatography using solvent X as themobile phase. One spot is observed after the plate is developed and visualized. The same unknown substance is re-analyzed using solvent Y as the mobile phase. This time, three spots are observed after the plate is developed andvisualized. Is the unknown sample a pure substance or a mixture? Explain your answer, including a possible reasonfor the different observations in the two experiments, The liquid sam pleis a mixtereSalvents *toseperate the camponents of the liquid5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it isBecause withn soluent Y t showspolar3sopotstoowaspacked into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull.The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and asilica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume thesame type of binding forces between the components and the stationary phase are present in both paperchromatography and column chromatography.)

5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it is packed into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull. The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and a silica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume the same type of binding forces between the components and the stationary phase are present in both paper chromatography and column chromatography.)

5. Column Chromatography works by the same principle as TLC however the silica gel is not plated on a plate but it is packed into a glass (or plastic column) and the eluting solvents runs down a column by gravitational pull. The mixture from question 1 (above) is analyzed by column chromatography using the same mobile phase and a silica gel stationary phase. Which component has the shorter retention time? Explain your answer. (Assume the same type of binding forces between the components and the stationary phase are present in both paper chromatography and column chromatography.)

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter15: Molecular Luminescence Spectrometry

Section: Chapter Questions

Problem 15.8QAP

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