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- A culture of S. cerevisea has an overnight OD of 2.3 (1.0 OD is approx 1.0x107 cells/ml) You will be plating 100µl onto agar and want the final count of colonies on the plate to be around 300 colonies. How much of the 2.3 OD culture must you use to get a 500µl subdilution (with sterile water), so that you have diluted enough to get approx 300 colonies per 100ulYou are given reference temperature 121 °C, Z value 10 °C, F0 15 min for killing Clostridium botulinum in commercial sterilization. Calculate F value when sterilized at 131 °C in canning processing.Is UVC disinfection of N95 face masks effective? Give two (2) important considerations for UVC disinfection of N95 face masks to be effective?
- Sulfur Indole Motility (SIM) Medium H2S produced, color and +/-: ______________________________ Indole present/Tryptophan hydrolysis, color and +/-: ___________________________ Motile or non-motile: _____________________________5 1 2 6 Testing Anti-Microbial Agents: Disk-Diffusion. Lab Report Fill out the zones of inhibition table with your data: Agent Tested Cleansing Spray 3 4 Foaming Handwash Hand Sanitizer Lysol spray Detergent Desinfecting zone of inhibition: (mm) E. coli 10mm 15 mm 20mm 0mm 9mm P. aeruginosa 8 mm 35mm 17mm 15mm 0 mm 1mm Hydrogen Peroxide Here, write a few observations about your experimental results. 25mm S. aureus 25mm 20mm 25mm 12mm 8mm 42mm What factors (at least 2) other than the identity of the agent being tested can influence the size of the zone of inhibition? NOT Were any colonies observed within the zone of inhibition? Even if you didn't have any, explain why you might see colonies in the zone of inhibition. Be specific about how that might happen? What follow up questions or conclusions do you have related to your data? (at least 2)Detergents that are good micelle formers often have strongantibacterial properties. Considering the structure of thecell membrane, suggest a reason for this antibacterialaction.
- The KD of a given objective lens is 1.5 μm/ocular unit, a microbe spans 3 ocular units. What is the cell diameter of this microbe?A research scientist is developing a new antimicrobial drug for a pharmaceutical company. Discuss at least 4 attibutes that this drug should posses in order to be an ideal antimicrobial drug.diameter AM=47MM TE= 27 MM both left hand side is ampicillin and right hand side is tetracycline With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against coli and S.aureus. S.aureus Antimicrobial agent Interpretive categories and zone diameter breakpoints, nearest whole mm sensitive Intermediate resistant Tetracycline >19 15-18 <14
- How does cotton plug prevent contamination of the medium? [Answer in not more than 2 sentences]Differentiate Streptolysin O from Streptolysin S. What titer is significant for streptococcal infection? Discuss the principle behind ASO titration method based on Neutralization reaction.Why is washing with Hank’s balanced salt solution with 0.5% antibiotic-antimycotic done for Splenocyte Preparation?
