6. Tim is observing xylem vessel elements under the microscope. He initially used the low power objective (LPO) to search for the vessel elements formed in his specimen (Fig.3 A), he. He then wanted to see the vessel elements in greater detail, so he used the high power objective (HPO). Although he didn't have any trouble in resolving the vessel elements using the HPO he wanted to try using the oil immersion objective (OI0). He noticed that he needed to use the fine adjustment knob to focus at two different groups of vessel elements one at a time (Fig. 5 B and C). He also noted that he cannot completely resolve the specimen under the O10 when it should appear clearer as shown by his instructor previously. A B Figure 5. Vessel elements developed from cassava pith in response to synthetic plant hormones under LPO (A) and OIO (B and C). a Explain why Tim can only focus on one group of vessel elements at a time when he used
Microscopic examination
The analysis of minute organisms, cellular organization of any biological structure, and composition of body fluids with the help of a microscope is known as microscopic examination. The magnification of specimens or samples under study helps in attaining a clearer picture of it.
Gram Staining
Named after Hans Christian Gram, a Danish bacteriologist, Gram stain is one of the most powerful staining techniques within microbiology. This technique was introduced in 1882 to identify pneumonia-causing organisms. The Gram staining technique uses crystal violet or methylene blue as primary staining colors to distinguish gram-positive from gram-negative organisms. Under a microscope, the gram-positive organisms appear purple-brown, retaining the primary color. Gram-negative organisms appear pink or red as they do not acquire the color of the primary stain.
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