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- Using the formula V₁D₁ = V₂D2, what volume of a 1/100 (10-2) dilution would you need to produce 100 mL of a 1/10000 (10-4) dilution? (Note: provide your answer in decimal format to three decimal places.)If a pharmacist added each of the following to 22-g packages of 2% mupirocin ointment, what would be the percentage strengths of the resulting ointments: (a) 0.25 g mupirocin powder and (b) 0.25 g of nonmedicated ointment base? (answer to two decimal places). answers: (a) 3.10% w/w (b) 1.98% w/wA volumetric flask made of Pyrex glass is calibrated at 20 °C.It is filled to the 100 mL mark with 35 °C acetone. After the flask is filled the acetone cools and the flask warms so that the combination of acetone and flask reaches a uniform temperature of 32 °C. The combination is then cooled to 20 °C. a) What is the volume of the acetone when it is at 20 °C? b)At the temperature of 32 °C, does the level of acetone lie above or below the 100 mL mark?
- i) Write down the equation derived from your Excel generated standard curve and describe its components; ii) provide the values of the absorbance data of the unknown sample (do NOT show the absorbance data of the glycine standards). Show all details of the working out of your calculation. Indicate all units! Provide the answer with two decimals precision iii) state the answer in a full sentence. (note: avoid mathematical symbols [=, +, -, etc] in your answer sentence).Below is a Beer's Law Plot for the concentration of a specific colored compound x. A solution of compound X is measured with the spectrophotometer to have an optical density of 0.60. a) What is the concentration of compound X in this solution. Answers must contain proper units for full credit. b) The absorbance of this compound was measured at 540 nm. Why was this absorbance chosen? 0.9 0.8 0.7 0.6 805 4 0.4 0.3 0.2 Absorbance at 540 mThe standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.
- a) A bottle is labeled Demerol (meperidine) 50 mg/cc. How many cubic centimeters are needed to give a 125 mg dose? b) Promazine HCL comes as 500 mg/10 ml. How many milliliters are needed to give 150 mg dose? c) Hyaluronidase comes as 150 U/cc. How many cubic centimeters for a 30 U dose?Determine the weight/volume of the chemicals needed to prepare the following solutions: a) 100 ml of 0.9% (w/v) saline (NaCl) b) 30 ml of 50% glycerol (v/v) c) Electrophoresis requires TAE, which is a specific mixture of Tris base, acetic acid, and EDTA. TAE is normally made as a 50X concentrated stock. Provide a recipe to make 40 ml of 50X TAE. The recipe for one liter of 50X TAE is as follows: 242g Tris base, 57.1 ml glacial acetic acid, 100 ml 0.5 M EDTAB. You will need approximately 1 ml of the 100 µg/ml bromophenol blue (which is your most concentrated standard) to generate your standard curve. Calculate how much bromophenol blue stock solution and how much water you will combine to accomplish this. Make 1 ml of this solution using a 100-1000 μl micropipetter. Use the equation C₁V1=C2V2 μl of 1 mg/ml stock + μl of water = 1 ml of 100 µg/ml bromophenol blue
- 1.0 ml of serum albumin (BSA) solution was precisely diluted to 100 ml with a buffer solution, and the absorbance at 280 nm was measured from this buffer solution at a distance of 1 cm from the light. The result was A=1.0. BSA is 0.1%(=0.1g/l). The absorptivity is e(0.1%)=0.667, so 1g/l solution gives an absorbance of 0.667. What was the original protein content? Report in the result unit mg/ml with an accuracy of 0.1 mg.Scott test is one of the methods used for screening for Cocaine in Forensic Chemistry. The multi step method consists of a) reacting the sample drug with cobaltous thiocyanate (Co(SCN)2) b) dissolving of the formed precipitate by adding HCl and c) extraction of coordination compound with Chloroform d) measurement of absorbance of extracted coordination compound at 627 nm What is the type of chemical equilibria involved in first step of the method? a.Solubility Product equilibria b.Bronsted Lowry acid – Bronsted Lowry base equilibria c.Complex ion formation d.Coordination compound formationA 5 ml vial of the hydrocortisone is having 2% (w/v) concentration. How many vials will be required, if we needed to prepare 30 ml of a 8 mg/ml oral hydrocortisone?