Which of the following is NOT true about heat fixation Select one: O a. Heat-fixed bacteria adhere better to the slide O b. Heat fixation requires using 95% methanol c. Heat fixation is carried out as the first step of many staining procedures O d. The heat fixed smear needs to be air-dried first

Which of the following is NOT true about heat fixation Select one: O a. Heat-fixed bacteria adhere better to the slide O b. Heat fixation requires using 95% methanol c. Heat fixation is carried out as the first step of many staining procedures O d. The heat fixed smear needs to be air-dried first

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter21: Infection Control And Medical Asepsis

Section: Chapter Questions

Problem 8CR

See similar textbooks

Related questions

Q: What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop…

A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…

Q: Gram Stain Results: Staphylococcus aureus (a Gram-positive coccus) Escherichia coli (a Gram-negative…

A: Staphylococcus aureus It is a Gram positive coccus bacteria. These bacteria have the very distinct…

Q: There is definitely survival of some microorganisms even after alcohol treatment. Without staining…

A: Bacteria are microscopic single-celled organisms that thrive in diverse environmental conditions.…

Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…

A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…

Q: The number of bacteria in the samples need to be quantified. The laboratory uses a specific protocol…

A: Introduction :- Bacteria are single-celled organisms with a small size. Bacteria can be found…

Q: For post mortem stain to occur ______must happen. A. Coagulation B. Hemolysis C. refridgeration…

A: For post mortem stain to occur pH shift to alkaline must happen.

Q: For a successful direct staining, one should A. use a minimal amount of water to speed up the…

A: Direct staining is the process to make slides using a single stain or dye.

Q: The sediment observed in stained blood smear caused by: a) Overstraining b) More Haematoxylin in the…

A: Here we have to explain about why sediment observed in stained blood smear.

Q: A medical technologist intern is preparing a blood agar medium on a hot plate while the other intern…

A: While working in the laboratory, laboratory hazards can take place unintentionally at anytime which…

Q: ... E Topic: laboratory Instrument Which of the following are TRUE regarding Which of the following…

A: INTRODUCTION The answers on the topic laboratory instruments are given below.

Q: Which of the following are true about a differential stain. There may be more than one answer!…

A: A microscopic organism made of one cell is referred to as a prokaryote. Prokaryotic organisms do…

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A: Gram staining may be a staining strategy utilized to distinguish and recognize bacterial living…

Q: Using a light microscope, samples can be viewed by which method (choose all that apply) O dissecting…

A: An optical microscope is another name for a light microscope.

Q: Select all of the following that represent examples of positive stains. A) nigrosin B) methylene…

A: Staining is a technique usually employed to enhance the contrast of specimens in microscopy. Many…

Q: .Leifson's stain is made up of tannic acid, basic fuschin stain prepared in alcohol base .A .B F .c…

A: Leifson's stain is a method used to identify the infections of the Helicobacter pylori bacterium.…

Q: Put the numbers in the space provided to indicate the order of Capsule Staining. If the statement is…

A: The primary function of capsule staining is to differentiate capsular material from bacterial cells.…

Q: During the preparation of the capsule stain, the slide used to spread the India ink/bacteria mixture…

A: Capsule stain is a type of differential stain which uses acidic and basic dyes to stain the…

Q: Gram staining is the most common initial test of unknown identification, explain in your own words…

A: The Gram staining is a typical process that is used to separate two groups of microorganisms based…

Q: bacteria (Mustard + 0.5% acetic acid or Mustard +1% acetic acid)

A: Different methods are used to prevent food from spoilage without changing its nutritional…

Q: Is is best to observe slides of bacteria stained with the acid fast stain under high dry…

A: The acid-fast stain is used to stain the acid-fast organisms. Acid-fast microorganisms have a thick…

Q: Give at least two other examples of samples that are best prepared using Smear Preparation…

A: Smear preparation technique: it is used to fix the samples onto the slides.

Q: If the stain is too red to the naked eye, what can be done to correct this? O that is normal O check…

A: Staining is a microscopy auxiliary method used to improve contrast in the microscopic image. Stains…

Q: Which of the following is true Select one: O a. Inoculating loop and needle are culture equipment…

A: Answer: CULTURE MEDIA = These are the microbiological culture which are used for the bacterial…

Q: A bacterial sample collected from a patient is processed using a Gram's stain. After staining, the…

A: Gram stain is a test that looks for bacteria in areas where an infection is suspected, such as the…

Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: a.) Give the purpose of heat fixation. b.) What can be observed in wet mounts or hanging-drop slides…

A: The Purpose of fixation of a smear is as follows: 1) Tissue Killing- As this will help to prevent…

Q: In case you forgot to identify and label the specimen, this can be easily troubleshooted since body…

Q: chemically defined media b. complex culture media

A: Media is a growth medium of solid,liquid and semi solid type. Diffarent types of media are used for…

Q: Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and…

A: Bacteria are small, microscopic and unicellular organisms that can live in variety of environment.…

Q: An inoculating loop must be flamed to uniform orange-hot from base to tip A. after culture is…

A: An inoculation loop is a tool used by microbiologists to transfer a small culture of microorganisms.…

Q: You collected a sample from a patient with a suspected STI. After Gram staining, you observed this…

A: The staining of bacteria is an important method of classifying the bacteria There could be multiple…

Q: In order to have a better visualization of the shape acid the fast stain, you should do the…

A: Use of carbol fuchsin on the bacteria and heating it p, makes the cell appears red, presence of…

Q: Select all of the following that represent examples of positive stains. Check All That Apply…

A: Staining is the common laboratory procedure which can be specific and general according to the use.…

Q: You can perform a specific staining method to confirm the purity on the culture. Describe this…

A: Staining methods are used to differentiate between two bacteria depends on their cell wall. To…

Q: With liquid media such as broth, is it possible to determine if the culture is pure using the naked…

A: A culture is a method by which microbes are allowed to reproduce and multiply in predetermined…

Q: Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big…

A: Petri plates, slants and stabs of Culture media such as Luria Bertani agar is used in microbiology…

Q: You have a patient sample and have done a Gram Stain. When you look on the microscope, this is what…

A: After a clinical sample is received in the laboratory, the first step often is to do a mandatory…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: If you prepared a tube with 10-2 dilution and from this 0.1 ml ia taken into 9 ml medium, the new…

A: Given Initial concentration 10-2 Volume taken = 0.1ml Volume added = 9 ml Find final dilution…

Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…

A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…

Q: A patient has subcutaneous nfection and the specimen is submitted on a swab . Explain why this is an…

A: Several biological tests involves testing of the the samples from the infected individuals body.…

Q: Gram straining procedure. Indicate the following information A. Shape........ B. Stained color…

A: On the basis of gram staining, we can differentiate between two types of bacteria, one with thick…

Q: Which of the following is true Select one: O a. Inoculating loop and needle are transfer equipment…

A: Culture medium is the primary production environment for biological products. The pilot or scale-up…

Q: a. STREAK PLATE METHOD: a. Pour melted NA or CWA, following aseptic technique in a petri dish and…

A: The reason for the streak plate is to acquire separated colonies from an inoculum by making areas of…

Q: Which of the following are true regarding UV disinfection? Mark all that apply. Water must be clear,…

A: Electromagnetic spectrum is the large range of light rays containing x rays, gamma rays, visible…

Q: at is the wavelength of maximum aborbance for the Bradford reagent in its blue form. at is the…

A: Bradford reagent in Bradford assay is used to to assess the amount of protein in a sample. The…

Q: Explain how bacterial cells would look in the gram staining procedure if the following mistakes were…

A: Microorganisms are those small organisms that are invisible to the naked eye and can be seen only…

Q: You can perform a specific staining method to confirm the purity on the culture. Describe this…

A: Microscopic samples are generally stained prior to analysis as it enhances their contrast and aids…

Q: Congo red is a red acidic stain and methylene blue is a l a bacterial smear with a mixture of eosin…

A: Eosin Methylene Blue (EMB) agar is both selective and differential culture medium. It is a selective…

Q: Which of the following methods is most sensitive for identifyingdifferent strains of a microbe?a.…

A: Microorganisms or microbes are small living organisms that can be observed with the aid of…

Concept explainers

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.

Question

Microbiology

Which of the following is NOT true
about heat fixation
Select one:
O a. Heat-fixed bacteria adhere
better to the slide
O b. Heat fixation requires using
95% methanol
c. Heat fixation is carried out as
the first step of many staining
procedures
O d. The heat fixed smear needs
to be air-dried first

Study of lifeforms too small to see with the naked eye, including bacteria, viruses and protozoans. Microbiologists may study bacteria, yeast, or other microbes to understand better their reproduction, life cycle, or role in causing disease.

Expert Solution

This question has been solved!

Explore an expertly crafted, step-by-step solution for a thorough understanding of key concepts.

This is a popular solution!

SEE SOLUTION Check out a sample Q&A here

Step 1

VIEW

Step 2

VIEW

Trending now

This is a popular solution!

Step by step

Solved in 2 steps

SEE SOLUTION Check out a sample Q&A here

Knowledge Booster

Learn more about

Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.

Similar questions

G When is it appropriate for gloves X + rface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod... 2 Exit rientation to Infection Prevention Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG IN
entic X G Which of the following are prope X elnterface/defaultui/player/modern.html?configuration=&preventRightClick=False&cc=en-US&ieCompatibilityMod.. 2 Orientation to Infection Prevention Exit Which of the following are proper disinfection steps? Check all the apply. O Remove organic matter O Disinfect only O Follow suggested contact time O Mix cleaning products to make the disinfectant more powerful O Disinfect O Disregard the product labels SUBMIT ENG 令中) IN 40 ho
Cite the purpose of each of the following reagents in a differential stainingprocedure.a. Primary stain:b. Mordant:Lab reportExPEriMEnt9 90 Experiment 9: lab reportc. Decolorizing agent:d. Counterstain:
Neutralization of residual disinfectant could be made by: a. Dilution if the concentration exponent is high b. Dilution if the concentration exponent is low c. Adding specific agent e.g. 3% Tween 80 d. Either a or c e. Either b or c
Briefly explain how the following can affect the quality of stained blood film; I) .The size of the blood drop used for the preparation of the filmi ii. A delay in smear preparation III). The angle of spreadiv). humidity of the laboratory environmentv). Pressure 2. Describe five characteristics of an ideal smear
Idenitfy the statements whether they are TRUE or FALSE 1. You should use P-20 micropipettes to transfer volumes for spread plating. 2. You should use P-200 micropipettes to transfer volumes for pour plating.
Vida works in the haematology laboratory of a smallhospital. The laboratory’s haematology procedure manualcontains instructions for staining blood smears and callsfor applying the stain for 2 minutes and the buffer for 2minutes. Vida stained three patient blood smears, but thesmears appeared bluer than normal. When she examinedthe smears microscopically, the cells also appeared blue,making it difficult to identify them.1.What can cause blood smears to stain too blue?2.Discuss factors that could have affected the stain results.
For which disease(s) is the Acid Fast stain diagnostic? (select all that apply) Strept throat Diabetes Tuberculosis O Leprosy o o o O
Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.
The following methods used for sterilizing an antibiotic solution. i. Dry heat sterilization ii. Microfiltration iii. Autoclaving iv. Desiccation Select one: O a. i and ii O b. i and iii O c. ii and iv O d. ii only
In the conduct of serial dilution, which of the following tools is NOT necessary? a. Agar plates b. Micropipettor c. Inoculating needle d. Alcohol lamp or Bunsen burner e. Test tubes or microcentrifuge tubes
What is known as sterile technique, prevents contamination of an open wound, serves to isolate the operative area from the unsterile environment, and maintains a sterile field for surgery? " Aseptic Technique O Handwashing Medical Asepsis O Surgical Asepsis The clinical instructor asks her students the rationale for hand washing.. The students are correct if they answered that hand washing is expected to remove: transient flora from the skin. O resident flora from the skin. all microorganisms from the skin. O media for bacterial growth.