Cat Noir was tasked to determine the concentration of a CuSO4 unknown solution. She prepared 5 different standards of CuSO4 using the table below as her guide. She ran the standards and the unknown solution in the UV-VIS Spectrophometer, and recorded the absorbances of each solution. Show linear equation derived from calibration curve and solution to concentration of the unknown solution below. Encircle final answer.

Cat Noir was tasked to determine the concentration of a CuSO4 unknown solution. She prepared 5 different standards of CuSO4 using the table below as her guide. She ran the standards and the unknown solution in the UV-VIS Spectrophometer, and recorded the absorbances of each solution. Show linear equation derived from calibration curve and solution to concentration of the unknown solution below. Encircle final answer.

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter10: Tlc Analysis Of Analgesic Drugs

Section: Chapter Questions

Problem 5Q

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To run a spectrophotometry experiment, begin by warming up the spectrophotometer and preparing the samples. Be sure to select the correct absorbance (X), then run a measurement on the sample solution. Follow up by running measurements on sample solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples. Partially correct (2 of 4 correct)
A series of six solutions was prepared at the given concentrations. Sample AB C D E F Concentration (mM) 50 100 | 150 | 200 300 400 The absorbance of each solution was then measured using a colorimeter. Sample A B C D E F Absorbance 0.25 0.50 0.75 1.00 1.50 2.00 What is the concentration of the solution with an absorbance of 1.50? C = mM
Construct a calibration curve based on the following data: Concentration Fe 0.100 0.500 2.00 4.00 7.00 (ppm) Absorbance 0.0122 0.0608 0.2322 0.455 0.5858 Use LINEST to find the slope and intercept and their uncertainties. Enter your answer as a number with no units to three significant figures. 0.085 0.037 b m 0.100 0.500 Sm Sb r2 0.958 0.037 Sy
How do I find Concentration? flask Calculate solution Concentration (M) Volume stock of 0.40 M CuSO4 (ml) Volume of flask (ml) Absorbance at 635 nm(a.u.) 1 2.0 10 .185 2 4.0 10 .362 3 6.0 10 .510 4 8.0 10 .674 5 10.0 10 .843
The spectroscopic data in the table is generated with five solutions of known concentration. Concentration (M) 0.0133 m= 0.0266 0.0532 0.106 0.213 Absorbance 0.1271 What is the intercept of the linear regression line? 0.08531 0.5388 1.069 Use a spreadsheet program, such as Microsoft Excel, to graph the data points and determine the equation of the best-fit line. 1.954 What is the slope of the linear regression line formed by these points? M-1
What are the differences between systematic and random errors and how do they effect accuracy and precision? In what circumstances would you use standard addition (versus a normal calibration curve) to determine the amount of an analyte in a sample? A urine sample, containing analyte Z is analysed by the standard addition method where 5 mL of the original sample was mixed with increasing amounts of a Z standard and each solution diluted to a volume of 50 mL prior to analysis. A plot of the final concentration of the standard in each of the 50 mL samples (x axis) versus The measured signal from the analysis of each 50 mL sample (on y axis) produced a straight line with the general equation: y = 44.72x + 4.06 what was the final concentration of Z in the 50 mL standard addition sample? what was the initial concentration of Z in the original urine sample?
■ True or False: in a TOF analyzer, a fragment of low mass reaches the detector first ▪ In GCMS when we know what analytes we are measuring we use not know what analytes are in the sample we use than scan. ▪ A) Scan; SIM ■ B)SIM; scan • C) SIM; SIM mode. When we do mode. SIM allows greater sensitivity
#9: This unknown contains 51.90 % C and 9.80 % H by mass with the remainder being an unknown element (you have to figure out what element). 100- Base 80 60 90 Relative Intensity 100 TRANSNETTANCE 40 20- O 4000 10 20 3000 30 peak 656 50 40 m/z REVENUTER 60 1300 peak 094 92-37= 90-35= C) 70 80 1000 90
The spectrophotometer is set at 600 nm. The absorbance of each cuvette is read. The data is shown below: Cuvette 1 is the clank Cuvette # ml albumin Absorbance 0. 0.2 .063 3 0.4 .163 4. 0.6 .187 0.8 .253 1.0 .289 Using this data, plot an Excel graph showing absorbance (y-axis) vs ml albumin (x-axis). Find the correlation coefficient (r). R2 > 0.99 = excellent data; r2 0.98-0.95 good data r2 0.94 -0.90 = fair data. How would you describe this data?- To find the relative sensitivity, for each of tubes 2-6 (neglect blank), perform the following operation absorbance (Lowry)/Absorbance (Biuret x 0.5 gram albumin (Biuret)/0.05 gram (Lowry) x 200 ml (Lowry)/100 mL (Biuret). This implifies to Relative sensitivity = 20 dilution factor x absorbance (Lowry)/Absorbance (Biuret). Report your relative sensitivity as an average of all 5 values.
1. Take your data home and use EXCEL to prepare a Beer's Law plot of the data from your standards (Trials 1-5). You should plot Absorbance on the "y" axis and concentration on the "x" axis. Include a print out of your graph with your report. 2. Add a trend line to your graph in the same way that you added the trend line to the graph you prepared in your density experiment (EXP 1). Be sure to show the equation the line on your graph. 3. Utilize your graph to determine the concentration of your unknown copper sulfate solution. Show how you did this on your graph. Unknown Concentration from Graph. M 4. Use the equation of the line on your graph to determine the concentration of your unknown copper sulfate solution. Show your work below. Unknown concentration from linear equation M
2. Add the absorbance of your Part III solution to the graph. Llse the of best fit of your qraph to calcuate its line concentration. Concentraton vs Absorbance 1.6 con c: 0.003M 1.4 1.2 0.8 0.6 0.4 0.2 0.0005 0.001 0.0015 0.002 0.0025 0.003 0.0035 Concentration Co.003M) Concentration you have calculated in 2 is CCrO, of Ag CrO4. calculate your experimental ksp of Ag CrO4. = [Cr O4]? Use it to 2. %3D ksp of AgCr Oy = CAg+] [CrO4] Absorbance te Out
A solution of FESCN2+ has a concentration of 1.00 x 10-M and an absorbance of 0.755. What is the concentration of an FeSCN2+ solution with an absodbance of 0.491? Enter your answer in scientific notation, with one decimal place, as follows: 2.3e-4 or 2.3E-4 Do not include units and note that 10 and x are NOT USED in this type of scientific notation. Question 3 of 26>»

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Cuvette	Volume 0.5000 M CuSO4	Volume distilled H2O	Absorbance
1	1.000 mL	4.000 mL	0.607
2	2.000 mL	3.000 mL	0.655
3	3.000 mL	2.000 mL	0.709
4	4.000 mL	1.000 mL	0.797
5	5.000 mL	0.000 mL	0.832
Blank	0.000 mL	5.000 mL	0.000
Unknown solution			0.684