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What is the description of the growth and growth pattern of the microorganisms in the pour plate method?
Type of Growth: Surface or Sub-surface?
What is the typical growth pattern of inocula?
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- What is the description of the growth and growth pattern of the microorganisms in the spread plate method?Type of Growth: Surface or Sub-surface?What is the typical growth pattern of inocula?What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable numberAfter streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?
- Using your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. List and draw the possible characteristics of an isolated bacterial colony that can be observed based on type of (a) margin, (b) elevation, (c) texture, and (d) optical property.What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?What is the advantage of the plating method over an electronic cell counting method in counting cells? Why does turbidity lose reliability at high cell concentrations when the culture reaches the stationary phase?
- In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldWhat method of bacterial measurement does this graph represent? Is it considered direct, or indirect? What phase of the growth curve can NOT be measured using this method?In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?
- What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar plate? What are the ways in which this can be prevented?A soil sample is placed in liquid and the number of bacteria in the sample determined in two ways: (1) colony count and (2) direct microscopic count. How would the results compare?a) Methods 1 and 2 would give approximately the same results.b) Many more bacteria would be estimated by method 1.c) Many more bacteria would be estimated by method 2.d) Depending on the soil sample, sometimes method 1 would be higher and sometimes method 2 would be higher.1) What are all of the different colony types that can be seen on a mixed-culture streak plate? 2) What kinds of colonies can be seen when mixed cultures of E. coli, M. luteus, and Serratia marcesens? 3) What is the range of colonies that may be seen with each mixed culture?