For an enzyme obeying the Michaelis-Menten equation with Km = 5 µM, kcat = 10 s-l and a total enzyme concentration of 1 nM, → Calculate Vmax. Calculate the substrate concentration at which v = 0.1 Vmax Calculate the substrate concentration at which v = 0.9 Vmax on your plot. What fraction of the enzyme is bound to substrate when v = 0.9 Vmax? Sketch a graph showing the Michaelis-Menten plot. Make sure you label the axes Label on the graph: i) the point on the graph at which S=Km ii) Vmax At low substrate concentration, v=Keat [Etot] [S] Km Circle on your graph where this equation applies. What name is used to refer to keat/Km? :Calculate keat/Km for this enzyme. How does this value compare with the fastest enzymes?
For an enzyme obeying the Michaelis-Menten equation with Km = 5 µM, kcat = 10 s-l and a total enzyme concentration of 1 nM, → Calculate Vmax. Calculate the substrate concentration at which v = 0.1 Vmax Calculate the substrate concentration at which v = 0.9 Vmax on your plot. What fraction of the enzyme is bound to substrate when v = 0.9 Vmax? Sketch a graph showing the Michaelis-Menten plot. Make sure you label the axes Label on the graph: i) the point on the graph at which S=Km ii) Vmax At low substrate concentration, v=Keat [Etot] [S] Km Circle on your graph where this equation applies. What name is used to refer to keat/Km? :Calculate keat/Km for this enzyme. How does this value compare with the fastest enzymes?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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