Q: What kind of stain would a pathologist use to visualise a chrondrosarcoma under the microscope? why…
A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Biological…
Q: Define fluorescent staining.
A: The advent of microscopy started with the development of optical microscopy. Then the level of…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: Why must heat or a surface-active agent be used with application of the primary stain during…
A: Microbiology is the study of microorganisms that are invisible to the naked eye. The microorganisms…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: What is the principle application of negative staining?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Why can’t nitric acid stains be removed by washing?
A: Nitric acid: it has the chemical formula HNO3. It is a conjugate acid of nitrate and a protic…
Q: For a stain to be considered differential, what must it do?
A: Differential stain uses more than two stains.
Q: What are the pros and cons of using the TCE stain-free method versus a traditional gel staining…
A: PAGE (polyacrylamide gel electrophoresis) is a well-known method for separating, detecting, and…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What are the principle and basic concepts of DIFFERENTIAL staining?
A: The simple dye is used in the technique of simple staining to highlight only the specific structures…
Q: In four steps, describe the simple staining procedure.
A: A stain is used to clearly view a specimen under the microscope. In simple staining, bacteria are…
Q: Differentiate between negative and positive staining, givingexamples.
A: These are the staining methods that are used to see bacteria under a microscope.
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: Defi ne dyes and describe the basic chemistry behind the process of staining.
A: Staining is a method by which contrast in a sample is enhanced usually at the microscopic level.…
Q: When it comes to simple stains, why is time so important? And what is the purpose of rinsing the…
A: Microbiology is a hugely important field in both basic and applied research. New and fascinating…
Q: What are the functions of a primary stain and a counterstain?
A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?
A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: What step normally associated with staining bacterial cells is omitted when the dimensions of cells…
A: Step 1 Gram staining is a technique of staining that is used to differentiate and categorize…
Q: What's the theory and conclusion of a acid fast stain?
A: It was Ziehl who originally devised differential staining procedures, that were later refined by…
Q: What is the secondary stain in both acid-fast stain methods?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: What is safe view dye for? Compare it with Ethidium Bromide.
A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…
Q: What is the purpose of the steam in spore stain?
A: Small tiny objects which are not viewed by the naked eye are called microbes. The microbes are seen…
Q: Why are chemical stains required for visualizing cells and tissues with the basic light microscope?
A: Cell staining is a technique that is used to better visualize cells and cell components under a…
Q: How does viability staining differ from stains like DAPI?
A: Staining is the technique in biology, which increases the visibility at the microscopic level. Dyes…
Q: Use the table to differentiate between agarose gel electrophoresis and SDS-PAGE based on the…
A: Agarose Gel Electrophoresis is the technique of separating DNA or protein. SDS-PAGE is the technique…
Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: Classify H and E staining?
A: Hematoxylin and eosin staining (H and E staining) is a widely used histological staining method used…
Q: What is the difference between Giemsa and Wright stain?
A: Staining is a technique used to enhance contrast in samples, generally at the microscopic level.…
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: What is the procedure for methylene blue stain on e.coli?
A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…
Q: What major advantage does phase-contrast microscopy haveover staining?
A: Phase-contrast microscopy is an optical microscopy technique used in cells. When the light passes…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle,…
A: Fungi consist of a broad class of organisms from single yeast to multicellular organisms like…
Q: Why is it incorrect to say that the GFP is a “staining” method?
A: Green fluorescent protein (GFP) is a protein composed of 238 amino acid residues (26.9 kDa) that…
Q: What macromolecules does DAPI stain? What are its advantages and disadvantages?
A: Genetics is the branch of biology that deals with genetic material like DNA, RNA, inheritance.…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: Discuss the importance of why flagellar stains must be freshly prepared?
A: Flagella Some bacteria, archaea, and eukaryotes whihcb are usually stayed to define as the microbe…
Q: Briefly describe the uses of Special stains.
A: Routine stain: It refers to the hematoxylin and eosin (H&E) staining that is used routinely to…
Q: Why do acidic dyes not stain bacterial cells?
A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: Name two fixatives used in staining?
A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…
Q: How do stains used for light microscopy compare withthose used for electron microscopy?
A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…
For What purpose is Giemsa stain used for?
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- What is the difference between Giemsa and Wright stain?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?