Q: Explain the reasons you would use a negative stain?
A: In a negative stain the slide is actually stained. This is because the negatively charged stain…
Q: What are the sources of error in performing Gram staining?
A: Bacteria can be divided into two categories namely gram positive(with thin layer of peptidoglycan)…
Q: Crystal violet is an example of what type of stain?
A: A stain is used to clearly view a specimen under the microscope. For staining, a smear of the…
Q: Explain the mechanism of staining for each of the stains used. Iodine Methyl green Nigrosin
A: The living cells can be seen under a microscope. The cells is not visible properly as it is…
Q: What is the principle application of negative staining?
A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…
Q: Why are basic dyes more effective for bacterial staining than acidic dyes?
A: Staining is a technique used in slide preparation to colour the specimen. With light microscopy…
Q: Why is the Gram stain considered a differential stain?
A: Prokaryotes are the primitive organisms that were the first to develop and harness life on Earth.…
Q: Principle and procedure for Mann’s methyl blue-eosin stain
A: Methyl blue stain Methylthioninium chloride or methyl blue is a stain use to highlight animal…
Q: In gram staining, could colors other than red be used as a counterstain?
A: A counterstain is a stain with color contrasting to the principle stain, making the stained…
Q: How is xylol used to clean the lens of a microscope? What may happenif xylol is constantly used for…
A: The microscope can be defined as the laboratory instrument which is utilized to examine objects that…
Q: Which step is the most crucial or most likely to cause poor results in the Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: Give the principle of Grams stain, Ziehl-Neelsen, and Kinyoun Staining methods
A: Ziehl neelsen staining principle The presence of mycolic acids in the cell walls of acid-fast…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: What are the most commonly used methods in staining? why?
A: Introduction Staining is a technique for enhancing contrast in material, usually on a tiny scale.…
Q: How are negative stains prepared?Name two applications for which this type of stain especially…
A: Negative staining is defined as a method of demonstrating the type of small objects (like bacteria)…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: What is the best stain for Gloeocapsa, India ink or methylene blue?
A: Gloeocapsa belongs to the group cyanobacteria. They have gelatinous sheath around the cells which…
Q: What are the functions of a primary stain and a counterstain?
A: The main stain and the counterstain must be of different colours so that the two forms of bacterial…
Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: What is the purpose of using a biological stain when microscopically examining cellular components?
A: Staining is a technique that is adopted to enhance the visibility of the specimens under study that…
Q: What is the advantage of the Kinyoun staining procedure over the Ziehl-Neelsen method?
A: Kinyoun staining is a technique used to stain bacteria. Specifically, acid-fast species of the…
Q: Why is it necessary to perform a negative stain to visualize capsules?
A: Introduction: Negative staining: This uses an acidic stain. The results yield a clear cell with a…
Q: What is an example of a negative stain?
A: The bacteria is the unicellular prokaryotic organism that maintains a definite shape since it…
Q: What are the principle and basic concepts of Simple staining
A: The simple staining doesn't give a lot of data about the cell separated from the bacteria'…
Q: What is the purpose of the steam in spore stain?
A: Small tiny objects which are not viewed by the naked eye are called microbes. The microbes are seen…
Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: Classify H and E staining?
A: Hematoxylin and eosin staining (H and E staining) is a widely used histological staining method used…
Q: Why must young cultures be used when doing a Gram stain?
A: Gram staining is a common technique that is used to differentiate bacteria into two broad groups…
Q: What is the mordant in the spore stain?
A: A mordant is any substance, which is used as a fixative to bind the dye on the substance.
Q: What is the procedure for methylene blue stain on e.coli?
A: Methylene Blue is a cationic stain, which binds to negatively charged parts of the cells, such as…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: what is the function of each one of the Gram-stain reagents?
A: Gram staining is the technique used to classify the bacterial species into broad groups based on the…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: What is the function of a mordant, and which reagent serves this purpose in the Gram stain…
A: An extensively used microbiological method of staining is called a Gram stain. It significantly…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: bacteria require staining before microscopic examination?
A: Bacteria are single-celled organisms with a small size. Bacteria can be found practically everywhere…
Q: why is gram staining important
A: Gram staining is a laboratory technique which is used to differentiate between different types of…
Q: Describe the procedure for performing a Gram stain. ▪ What is the procedure? ▪ How does each…
A: Bacteria are ubiquitous microscopic , one celled organism which possess variety of shape ,…
Q: What is the type of gram stain?
A: Gram stain or Gram staining, also called Gram's method, is a method of staining used to classify…
Q: What color wouod gram negative cells appear if the decolorizing step was left out of staining…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: do we need to stain microorganisms?
A: The study of microorganisms is termed microbiology. Microorganisms is microorganism, fungi, archaea…
Q: What type of chromophore is associated with a negative stain?
A: Bacteria are unicellular prokaryotic organisms that maintain a definite shape since they possess the…
Q: Why do acidic dyes not stain bacterial cells?
A: Stains may be divided into two classes based on the nature of their charged group Basic/ cationic…
Q: How can the thickness of the smear affect the staining procedure?
A: Smear can e defined as the spread of cells in a layer to observe it under microscope. Since, cells…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
Q: How do stains used for light microscopy compare withthose used for electron microscopy?
A: The micro-organism is the organism that is too small to be seen with the naked eye. It is viewed by…
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- What is the difference between simple and differential stains? Give an example of a differential stain.What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?
- What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary stain in the Ziehl-Neelsen method?Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the stain affect the staining of bacteria? Can dyes other than methylene blue be used for direct staining? Briefly explain.