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How does a contaminant such as salt affect the absorption of light by a spectrophotometric sample?
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- What are the light sources used in UV-vis spectrophotometry.The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?What are the underlying physical principles of paper chromatography? What is spectrophotometry, the essence of it?
- What is the total magnification possible with a 10x objective?Explain the relationship between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.Define each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?
- Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.Why is it important that the standard curve you create in biological analyses with spectrophotometry is measured using specialty cuvettes?Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement or during the transfer to the spectrophotometer?