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Explain the relationship between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.
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- Answer the following questions: 1. Give the general steps in the use of a spectrophotometer. 2. Define the following: a. ) Optical Density b.) Transmittance c.) Beer's LawDefine each of the following terms: A) What is resolution and how is resolution related to the wavelength of light used to illuminate the sample? B) What is the magnification of the specimen if you are using a 40x objective and a 10x eyepiece? C) How is the numerical aperture (NA) of a lens related to its ability to gather light from a specimen?What are the light sources used in UV-vis spectrophotometry.give ans
- What is the reason for the decrease in the ABS value over time when measuring in the spectrophotometer?Explain the underlying physical principles in the creation of image contrasts for the following two processes of image contrasts for the following two procedures. (a) Magnetic resonance tomography (b) X-ray-computed tomographyUsing the Field of View (FoV) formula: (Low Magnification/High Magnification)= (High Field of View/Low Field of View) determine the FoV in microns for all other objective lenses (4x,10x,40,100x) given that the diameter of the 4X scanner FoV is 3.2 mm Please, explain with the formula how it was used and how values were added
- In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?a. The scale of a spectrophotometer extends from 1 to 100% T, what are the values of these two extremes in absorbance? b. Convert the 98%T to absorbance
- a) Describe the technique Of Atomic Force Microscopy under the following headings: (i) Basic principle of AFM and overview of operations (ii) Modes of A FMIn spectrophotometry, what is the role of the green background in the set-up? What does it represent in the actual spectrophotometer?Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %