In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.
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In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3, oil immersion noil=1.52)? Clearly show your calculations.
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- Complete the following table by calculating the total magnification of the different objectives of the microscope with the given eyepieces. OBJECTIVES OCULAR Designation Magnification Focal Length Numerical Aperture (NA) Final magnification with: 10x 15x 25x Scanner 4x 46mm 0.10 LPO 10x 16mm 0.25 HPO 40x 4mm 0.55 OIO 100x 1.8mm 1.30Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Question: What is the total magnification of the image under microscope? Show your solution.Using a good compound light microscope with a resolving of 0.3 micro meter a 10x ocular lens ,and a 100 x oil immersion lens, would you be able to discern two objects separated by 0.4 micro meter?0.2 micro meter? 300 neno meter ? Explain your reasoning.
- What is the magnification of the following drawing of a specimen extending one- third of the medium power field (1.8mm) of a compound microscope? 10.8 cmCalculate the diameter of the field of view for each total magnification on your microscope in millimeters (mm) and then convert this value to micrometers (um): 4.5 mm = 4,500 um Scanning (40X): 1.8 mm x 100X/40X = Low power (100X): FOV diameter 1.8 mm = 1,800 um High power (400X): 1.8 mm x 100X/400X = 0.45 mm = 450 um Oil immersion (1000X): O.18m 180 um 1.8 mm x 100x/1000X = Draw and estimate the length of a single Euglena (high power) and Paramecium (low power): Paramecium Euglena total magnification total magnification 400x yoox FOV diam. FOV diam. um uni length length umWhat is the diameter of the field of view (DFV) of a 1000x objective lens if the DFV of a 400x objective lens is 500 μ? Express your answer in mm.
- Given: The used ocular objective while taking this image, has magnifying power of 6x The used objective while taking this image, has a magnifying power of 100x The used stage micrometer has spaces graduation of 01 mm each Ten (10) graduations pn the ocular micrometer conincided with two (2) graduations on the stage micrometer Questions: What is the total magnification of the image under microscope? Show your solution. What is the distance of one ocular division? Show your solution. What is approximate cell length of Cell A, Cell B, and Cell C? What is approximate combine cell length of Cell A and Cell B? Show your solution. Remember: Always measure the cell length on the longest middle section of the cell!The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?The FOV on low power of a microscope is measured to be 4.5mm. Using the chart below and the above listed formulas calculate: FOvx = FOvlow X Maglow Magx S.S. = FOvx # times the specimen fits across the FOv Total Mag = Ocular lens x objective lens a) What is the FOV on High Power? (Magnification on High power is 40x) Can you please include the units too. asap please.
- Differentiate between the limit of resolution of the typical light microscope and that of the unaided human eyes the relationship between the depth of field and magnification is that When magnification increases, the depth of field decreases. Name two procedures (steps) you should do to achieve the maximum resolution when using the oil immersion lens State the relationship between the working distance of an objective lens and its magnification power “The coarse focus knob can be used to adjust the focus when using any of the objective lenses”. Is this statement true or false? If it is false, please correct it. When using the oil immersion lens but you cannot focus, what should you should not do? And what you should do? What does this statement mean: “Your microscope is parfocal”? What is the significance of condenser lens? Multiple choice question: The most useful adjustment for increasing image contrast in low power magnification is _______ Closing down the diaphragm Closing one…If the total magnification of a microscope is 700X and the ocular lens magnifies by a factor of 10x, what is the magnification of the objective lens that was used? Paragraph BIUthe figure shows the result of the spectral analyzer for paper spectrophotometer without any transparent paper and with transparent yellow color paper. observe and discuss the intensity and the wavelength for both results.