In an experiment of analysis of bovine serum albumin the spectrophotometer will be set to measure the light absorbed at 595 nm only, rather than a variety of different wavelengths of light. If you have measured the light absorbed at 580 nm for all of samples instead, how does it affect your results and consequently final result? Explain your answers in details.

In an experiment of analysis of bovine serum albumin the spectrophotometer will be set to measure the light absorbed at 595 nm only, rather than a variety of different wavelengths of light. If you have measured the light absorbed at 580 nm for all of samples instead, how does it affect your results and consequently final result? Explain your answers in details.

Principles Of Radiographic Imaging: An Art And A Science

6th Edition

ISBN:9781337711067

Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac

Chapter30: The Art Of Image Critique

Section: Chapter Questions

Problem 7RQ

See similar textbooks

Similar questions

You have carried out an experiment using the spectrophotometry concept. A solutioncontaining compound X is mixed with reagent 1 and then reagent 2. This mixture produces ablue colour whose absorbance (A) could be read at 550 nm. The results are shown below. If the standard solution (compound X) used have a concentration of 1 mM:1. Calculate the quantity of the standard compound X in μmoles for each test tube (1-7).
Consider the fluorescence spectra for bovine serum albumin (BSA) below. One solution contains phosphate buffered saline (PBS), while the other contains 1M HCL. Why does the fluorescence change the way it does? I.e., why do we observe a higher peak and a shift to the left under the presence of acid?
Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %
This question is based on Bovine Serum Albumin I would like help on answering letters a-c. This lab examines the relationship between the absorbance of light by a solution at 595 nm and the concentration of the Coomassie Blue dye-BSA protein complex in the solution. State whether the following descriptions of the lab experiment are valid or not, and explain why you say Yes or No: a. The experiment would be significantly more accurate if absorbance readings were recorded for a range of wavelengths, not just for 595 nm b. The experiment has limited accuracy because it does not account for the absorbance of light by the other components (components that are not the dye-protein complex, such as excess dye that is not bound to any protein) of the solution. c. The absorbance reading measures practically all the protein content in the solutions
In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.
The field of view (FOV) is the entire circular image we see when looking into the eyepiece. The diameter of the FOV gets smaller as we increase magnification. It can be measured by using a stage micrometer like a ruler, measuring from edge to edge. Notice that the stage micrometer is 1000 microns (µm) in length, and the field of view under the lowest magnification is 5000 µm. Describe how we measure it?
in basic spectrophotometer technique, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 CM-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2.
In basic spectrophotometer, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of a light beam is transmitted through 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 0.025 mg/mL. and compare between 1 and 2
From the basic spectrophotometer technique, 1. Calculate the concentration of bovine serum albumin (BSA) if 40% of light the beam transmitted through a 2 cm of the cuvette (assume the molar absorption coefficient, e is 43,824 M-1 cm-1). 2. Calculate the %T, if the concentration of BSA in a 2 cm of cuvette is 25mg/mL. Compare between 1 and 2.
A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the Amax of Ben, and 0.463 at the Amax of Jerry. STD CURVE BEN Amax Ben Amax Jerry STD CURVE JERRY Amax Ben Amax Jerry CONC (mM) ABS ABS CONC (mM) ABS ABS 100 0.074 0.025 100 0.037 0.074 200 0.148 0.057 200 0.081 0.148 400 0.284 0.103 400 0.164 0.284 800 0.607 0.218 800 0.287…
In etanol determination by specrophotometry, explain the purpose of the negative controls included in a protocol, what do they control for? (What will you learn if the negative control gives an unexpected result. i.e. returns a positive result?)
his is the result of an experiment in a spectrophotometer. please explain the graph (red and green one)