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- Are there technologies that can help make Bacterial Culture and Sensitivity done faster?Discuss how DNA extraction of microbial DNA directly can be done from infected tissue through an optimized protocol for use in nanopore sequencing.Why is pure culture and culture collection important in identifying novel species of bacteria?
- What is the difference between complete medium and minimal medium? How are complete media and minimal media to which one or more nutrients have been added (selective media) used to isolate auxotrophic mutants of bacteria?explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. (1) Restriction Fragment Length Polymorphism (RFLP)(2) Pulsed-Field Gel Electrophoresis (PFGE)(3) Whole Genome Sequencing (WGS)(4) Gram Staining(5) Biochemical Reactions: Multiple Tube Fermentation Technique(6) Biochemical Reactions: IMViC TestHow can single-cell genomics be used to addressmicrobial dark matter?
- Describe how specimen collection is performed using aseptic techniques to isolate medically significant bacteria and identify these clinically important bacterial species, Escherichia coli, Streptococcus pneumonia, Haemophilus influenzae and Streptococcus pyogenes by using different staining techniques and biochemical tests. Evaluate the effectiveness of different biochemical techniques used to identify different bacterial species.How can a suspect bacterium be fingerprinted?What is the advantage of using Molecular data compared to plated culture or biochemical techniques in identifying bacterial species, especially for oral microbes ?
- Determining the genetic profile of a bacterium for its identification is a part of which step of the identification process?What is the difference between culture-based technique and culture-independent techniques based on the principle behind the method?Describe how would you determine the following using a real-time PCR instrument. A) The quantity and quality of a DNA sample. B) Whether an individual had cystic fibrosis from a genomic DNA sample. C) If several bacterial pathogens were present in DNA extracted from a food sample.