i have a gene (3222bp) i want to put it into a expression vector (pet 28b+),when i did pcr i flanked my insert with EcoRI and HindIII restriction sites,how do i ensure that i insert the gene in the correct orientation in the vector,so that translation of the gene into a protein occurs.if the gene is inserted in the wrong direction would that mean i cant translate or would it just give me the wrong protein produced. also is the vector the one providing the start and stop codon ,and if so how do insert my gene so that it has a start and stop condon in the right fram

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Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.