If you have a mixed (Gram positive and Gram negative) sample, and you complete a Gram stain, and all the cells are purple at the end of the stain, what have you done wrong? forgotten safranin forgotten crystal violet forgotten decolorizer forgotten iodine everything worked perfectly

Q: Let's assume that you were teaching the Gram stain to four students, and each student did one thing…

A:

Q: A microscope that has a total magnification of 1,500× when using the oil immersion objective has an…

A: The microscope is the major tool in the identification of a microorganism after its isolation. The…

Q: layer

A: The crystal violet eventually escapes from cytoplasm of the cell, if left too long for…

Q: Which stain is most frequently used to distinguish differences between thecell walls of medically…

A: The cell walls of bacteria are made up of peptidoglycan- a layer of polysaccharide chains cross…

Q: If the iodine step in a Gram-staining procedure was omitted, what color would you expect…

A: Gram staining is a technique which is used to identify the bacterial cell type, whether it is gram…

Q: The acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with…

A: Mycobacteria are immobile, slow-growing (doubling time = 24 h), rod-shaped, gram-positive bacteria…

Q: An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and…

A: Introduction: Acid-fast staining is a type of differential staining technique that was first…

Q: In Gram staining technique, Decolorizing agent step should take Select one: O a. 60 seconds O b. 30…

A: Usually, Acetone is used as a decolorizer but some prefer ethanol and acetone in a 1:1 ratio.…

Q: Move the images of bacterial cells into the chart to illustrate the color of gram-positive and…

A: Gram staining is a technique used to differentiate between two groups of bacteria namely…

Q: Gram staining: What color would a Gram negative cell be if you forgot to wash with ethanol and why?…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: What is the importance of Gram-staining

A: Gram staining was created by Christian gram  Bacteria with Peptidoglycan in their cell walls are…

Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…

A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…

Q: The Gram Stain Build the gram negative structure

A: Gram stain is a type of stain that is used to identify the structure of bacteria. The stain itself…

Q: -What are the steps of gram staining -Which of the four steps is the most important and why? - What…

A: Based on the chemical and physical characteristics of their cell walls, the Gram stain is an…

Q: The bacterial cells in the image below were stained with a high concentration of carbolfuschin for…

A: Carbol fuchsin, carbolfuchsin, or carbol fuchsin is basically a mixture of "phenol" and basic…

Q: The Gram Stain is able to stain bacterial cells differently depending upon the structure of their…

A: Bacterial cell wall is made up of peptidoglycan. it prevents the cell from bursting when it absorbs…

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique used for staining the microbial samples. It was performed by the…

Q: list 4 advantages and 4 limitations of the Gram staining process

A: Gram staining process is the procedure which is uses differential gram stain which has two dyes to…

Q: The counterstain in the Gram stain is: A basic dye A negative stain A mordant An acid dye Necessary…

A: Staining is a method for enhancing contrast in material, usually on a tiny scale. Histology, as well…

Q: A new lab recruit performs their first Gram stain on a slide containing both G(+) and G(-)…

A: Gram staining is a common technique used to differentiate between gram positive and gram negative…

Q: what is gram staining

A: Bacteria is a vast group of microorganisms that consists of various shapes, sizes, structure and…

Q: If you forgot to apply the safranin counterstain while performing a Gram stain, which outcome would…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: If the decolorizing step was omitted in the Gram-staining procedure, what color would you expect…

A: Gram staining is performed on the basis of differential characteristics of the cell wall in…

Q: Stains Primary Stain Mordant Destain Secondary Stain Diagram of Stain Simple Stain N/A N/A N/A Gram…

A: This question is regarding different staining techniques of microorganisms.

Q: You and a partner conduct the acid-fast staining using the same two bacteria. You know that there…

A: Acid-fast staining is a differential staining technique that proves to be an important diagnostic…

Q: Define the following terms: a.Simple staining b.Complex medium c.Defined (synthetic) medium

A: Staining is used to visualise the bacteria i clinical specimen under a light microscope. Bacterias…

Q: The specimen for an electron microscope is always a. stained with dyes c. killed b. sliced into thin…

A: Electron microscope: It is a microscope which obtains a high-resolution image of non-biological as…

Q: - After you are finished, list each step for the Gram staining procedure. - Which of the four steps…

A: NOTE: AS PER OUR GUIDELINES WE NEED TO ONLY ATTEMPT FIRST QUESTION AND ITS PARTS , PLEASE RESUBMIT…

Q: Using the acid-fast stain's name as a guide, what would be a logical alternative name for the Gram-…

A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…

Q: observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram…

A: Microscopy is the technique which is used to magnify  small microorganisms and small specimens . It…

Q: An acid-staining method was performed on a bacterial smear using the dyes carboifuchsin and…

A: Acid Fast staining is used to detect the presence of acid-fast bacteria e.g. Mycobacterium…

Q: You have a microscope with a resolution of 0.5 um. Your bacteria on your sample are 0.3 um apart on…

A: The light microscope is a versatile instrument used to study a wide variety of types of specimens…

Q: Microscopic samples can be stained. the stains are used to increase and improve O resolution and…

A: Staining It is defined as a technique through which the cells or thin sections of biological tissue…

Q: This part of the staining process helps the primary stain to remain in the cell during…

A: Staining of biological samples is a very useful process for detailed observation and measurement of…

Q: Which step in the Gram stain procedure is most prone to error? If done incorrectly, how might that…

A: The differential stain is used to distinguish between different structures and cellular components.…

Q: You use gram staining procedure to stain a bacterium that lacks a cell wall. What color will the…

A: Gram staining is a special technique in which bacteria are stained with gram stain. It is used to…

Q: move the images of bacterial cells into the chart to illustrate the color of gram-positive and gram…

A: answer given below

Q: The Gram stain works because of differences in the O 1) cell membranes O 2) genetic characteristics…

A: Answer 1 cell membrane

Q: After performing the gram stain you observe your result as gram negative. To help confirm this…

A: Gram staining is a technique to identify bacterial stain gram-positive or gram-negative. It can be…

Q: You partner stained bacterial cells and saw only the background and not the actual cell was stained.…

A: Staining technique is a method in which recognition and visualisation of structure ,  arrangement of…

Q: After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple…

A: steps that are included in the Gram staining are- applying a primary stain (crystal violet) to a…

Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine  C.…

A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…

Q: What are the expected result after gram staining?   a. Crystal Violet = b. Gram Iodine =  c.…

A: Gram staining is a type of staining technique used to differentiate bacteria based on their cell…

Q: why is gram staining important

A: Gram staining is a laboratory technique which is used to differentiate between different types of…

Q: Explain how bacterial cells would look in the gram staining procedure if the following mistakes were…

A: Microorganisms are those small organisms that are invisible to the naked eye and can be seen only…

Q: employ a chemical (using a reagent, not a physical method) mordant? Gram stain; Acid-fast stain.…

A: The main function of modern in staining procedure is to retain the original stain or dye. It is a…

Q: You accidentally switch the Crystal violet and Safranin steps during your Gram stain. When you look…

A: The gram-positive and gram-negative bacteria can be differentiated based on their cell wall elements…

Q: Imagine you accidentally applied water during the decolorization step instead of ethyl alcohol.…

A: Gram positive bacteria has thick peptidoglycan layer and thin outer membrane. Gram negative bacteria…

Q: The gram stain is a ______________ stain in which a decolorization step occurs between the…

A: Gram stain is a popular microbiological staining technique for identifying and characterising…

Q: What is the identification of the unknown bacteria

A: Tetrad form are only seen in Gram positive cocci bacterium.

Microbial Isolation

Isolation of the microorganisms in microbiology is the process of developing a pure culture by separating one species of microbe from a mixture of many other species. A pure culture is a culture that contains only one species of microbe.