In the "biochemical assay of b-galactosidase activity", what is o-nitrophenyl-b-D-galactosidase (ONPG) used for? O When ONPG is cleaved by b-galactosidase, we are able to assay b-galactosidase activity. O It provides essential nutrients for bacterial growth. O It partially disrupts the cell membrane to allow cellular proteins to diffuse out of the cell. O ONPG cleaves the b-galactosidase that is made by the lac operon so we can see how much activity there is in the cell. O ONPG cleaves galactose so we can measure how much lactose there is in the cell.
Q: This question is based on vertebrate structures. How is the terrestrial environment different from…
A: An ecosystem is a natural community of living beings that deals with the external environment and…
Q: The bacterium Dontuplodemeus tocheggus has a generation time of 20 minutes. Starting with one cell…
A: Bacteria are a family of rod-shaped bacteria that are often found in soil or water. They are known…
Q: answer 6, 7, 17 that would fit to the puzzle
A: Cell biology is an area of biology that focuses on understanding the composition, division,…
Q: why do patients with von Gierke disease are still able to perform glycolysis given…
A: Introduction A cytoplasmic mechanism called glycolysis converts glucose into two molecules with…
Q: What is the importance of sllid width in fluorescence spectroscopy
A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.
Q: Differentiate budding binary fission, multiple fission. Give one organism example for each.
A: Introduction Reproduction is process by which organisms gives birth to offsprings. Reprpduction may…
Q: Discuss how environmental changes 65mya allowed mammals to diversify and reptile diversity to…
A: Introduction:- Evolution of living organisms on the planet Earth occurs continuously from the…
Q: At what stage of meiosis (I or II) does crossing-over occur?
A: Crossing-over is a process that occurs during meiosis (I or II) whereby homologous chromosomes…
Q: What are 3 characteristics that all types of connective tissue share?
A: Three man characteristics of connective tissue 1. Connective tissue are mesodermal in origin 2.…
Q: A scientist compares the plasma membrane composition of an animal from the Mediterranean coast with…
A: Introduction The plasma membrane, also known as cell membrane is the external layer of the cell that…
Q: The amino sequence of a protein is: alanine, leucine, valine, threonine. This information reveals…
A: The main structure is the most fundamental kind of protein structure. An easy, linear chain of amino…
Q: How do the pathologic changes and clinical manifestations of syphilis differ during the incubation,…
A: The spirochete Treponema pallidum causes syphilis, an infectious venereal disease. The transmission…
Q: why dp patients with von Gierke disease suffer from hypertrophy of the liver due to excess glycogen…
A: Introduction :- In Von Gierke disease, the body is unable to break down glycogen. The muscles and…
Q: Our current concept of the plasma membrane is called the fluidmosaic model. Why?
A: The most fundamental unit of life, a cell is made up of numerous membrane-bound compartments called…
Q: Identify the organelle most likely responsible for each cellular job within skeletal muscle a)…
A: Skeletal muscles are those which are connected with the skeleton and are voluntary in nature. These…
Q: Use the following sequence data to assign haplotypes and build a haplotype network for a 200 bp…
A: In its broadest meaning, a haplotype refers to a collection of DNA variants along a chromosome that…
Q: what are the 2 componenets that make up trhe extracelluar matrix?
A: The extracellular matrix surrounds the cells and tissues. It helps in cell movement, communication…
Q: Distance from your knees to the ground Distance from your belly button to the ground Distance from…
A: There is a cosmic constant called the ‘golden ratio’ which South African researchers say governs the…
Q: Answer quickly fast don't copy from Google otherwise downvote
A: We know that The plasma membrane is the regulator of movement of different molecules in and out of…
Q: You want to pipette 250uL of a sample in the lab. Which pipetter do you use? O p20 O p200 Op2 O…
A: Pipetter It is a laboratory tool commonly used in chemistry, biology and medicine to transport a…
Q: The cells shown in the image are from a rare species of plant and illustrate different stages of…
A: Cell division is an important process that helps to keep the cells growing in number. This enables…
Q: List five proteins of the myofilaments and describe their physical arrangement.
A: In muscle cells, myofilaments are the three protein filaments of myofibrils.
Q: Scientists often say “correlation does not equate to causation”. What do you/we/they mean?
A: Q.Scientists often say “correlation does not equate to causation”. What do you/we/they mean?
Q: What is your favorite muscle in the human body and what the action of that muscle is?
A: I think the most important, exciting and basic muscle which is present in human body is Cardiac…
Q: Which branch of the autonomic nervous system is responsible for penile erection? Ejaculation?
A: Introduction The nervous system is the main controlling center of the body which controls all…
Q: Why are most marine photosynthesizers unicellular?
A: Introduction: A single-celled organism is a unicellular organism, as opposed to a multicellular…
Q: What are the advantages and disadvantages of using benzodiazepine hypnotics versus non -…
A: Introduction Benzodiazepines are a class of drugs that can treat a range of conditions. Familiar…
Q: Fill in the blank. The style is No answer text provided. O Diploid O Haploid
A: Answer : First option is right - No answer text provided . Style - This is the…
Q: E3
A: The development of the embryo and of the fetus during the gestation period is known as prenatal…
Q: 3. Are animal cells and plant cells eukaryotic or prokaryotic? Why do you say so? 4. What structures…
A: Prokaryotic cell is that cell which does not have true nucleus or membrane bound organelles.…
Q: Based on your experience in the histology lab, examine the image below and identify the correct…
A: Respiration is a process by which oxygen found the lungs is carried by the blood to the tissues and…
Q: INSTRUCTION: Give the principle or significance of the following practices: 1. Flaming the mouth…
A: INSTRUCTION: Give the principle or significance of the following practices: 1. Flaming the mouth…
Q: Choose the best options, by dragging and dropping the colour coded text boxes below, that fill in…
A: Operon is transcription unit of prokaryotic genome with consist of many contiguous structural genes…
Q: From the ectodermal epithelium of the lining of the upper part of the intestinal fossa of the human…
A: Introduction :- Skin, sweat glands, skin sensor receptors, and hair follicles are just a few of the…
Q: 4. The following applies for humans and not birds? A. lack of lymph nodes B. somatic DNA…
A: Recombination is the production of new DNA molecule (s) from two parental DNA molecules or different…
Q: What is nomenclature
A: Later, Linnaeus divided all living organisms into two kingdoms: Plantae and Animalia. Whittaker…
Q: A woman 25 years old after a month after childbirth appealed to a doctor with a complaint about the…
A: The process of lactation results in the production of milk in humans. The mammary glands, found in…
Q: Give an example of a secretion by an endocrine gland and a secretion by an exocrine gland.
A: The ductless gland which secrets specific chemical substances and releases them directly into the…
Q: Define the two alternatives of bisexual reproduction-parthenogenesis and hermaphroditism, and give a…
A: Sexual reproduction is the process of creating persons from gametes. The most popular type of sexual…
Q: Which of the following statements is true regarding pili and flagella assembly? O Pili and flagella…
A: Pili are the short and straight hairlike structures of prokaryotic cells present on their outer…
Q: Give five types of locomotion of invertebrates and describe each.
A: Locomotion is the act of propelling an animal forward and moving from one location to another.…
Q: Explain how an individual’s phenotype may be determined by multiple genes that interact with one…
A: The mendelian inheritance includes a single gene with two alleles for each trait.
Q: and permanent. Why is ected into the dermis
A: So, this question is all about Tatoos & there location in which it's dye is being injected.…
Q: 1. Name the five types of white blood cells, and state a function for each type.
A: Introduction A component of our immune system that defends our body against infection is called…
Q: Distinguish between the genotype and phenotype of a given genetic trait.
A: A genetic trait is the feature of a living being that is regulated by genes.
Q: Label name biology
A: Introduction: The respiratory system constitutes a group of organs concerned with breathing…
Q: Highlights name
A: We can say that In the given image, we are shown a particular type of epithelium tissue. Epithelium…
Q: A cellular process is shown in stages from left to right in the image. Which of the following is the…
A: The correct description of its function is- B. Endocytosis, uptake of nutrients
Q: Coprinus-a small mushroom. Draw at a low enough power to see the overall central structure and…
A: The tiny genus Coprinus, which produces mushrooms, includes Coprinus comatus, also known as the…
Q: How did the diverse feeding appendages of arthropods arise?
A: Feeding appendages are the appendages which got modified to aid in feeding of organism. Arthropoda…
Step by step
Solved in 2 steps
- GTP-y-s serves as an analog of GTP that cannot be hydrolyzed any further. How would a Co-IP experiment differ between G- alpha & G-beta proteins in the presence of GTP-y-a and GDP?Measure the uptake of leucine by epithetial cells of the mouse intestine. Measurements of the rate of update of L-leucine, D-Leucine, and L-valine , with and without Na+ in the assay were perform and yield different results (see table below). A) What can you conclude about the properties and mechanism of leucine transporter? B) Would you expect L-leucine uptake to be inhibited by Ouabain, which is a cardiac glycoside drug treatment?CTP synthetase catalyzes the glutamine-dependent conversion of UTP to CTP. The enzyme is allosterically inhibited by the product, CTP. Mammalian cells defective in this allosteric inhibition are found to have a complex phenotype: They require thymidine in the growth medium, they have unbalanced nucleotide pools, and they have an elevated spontaneous mutation rate. Explain the likely basis for these observations.
- . Mutants of Neurospora crassa that lack carbamoyl phosphate syn- thetase I (CPS I) require arginine in the medium in order to grow, whereas mutants that lack carbamoyl-phosphate synthetase II (CPS II) require a pyrimidine, such as uracil. A priori, one would expect the active CPS II in the arginine mutants to provide sufficient carbamoyl phosphate for arginine synthesis, and the active CPS I in the pyrimidine mutants to "feed" the pyrimidine pathway. Explain these observations.A Leu →Ala mutation at a site buried in the core of the enzyme lysozymeis found to be destabilizing. Explain the observed effect of this mutationon lysozyme stability by predicting how enthalpy (ΔH°), conformationalentropy (ΔS°peptide), and the hydrophobic effect (ΔS°solvent) are expected to change for the mutant compared to wild-type lysozyme. Explain how ΔG°for unfolding is affected by your predicted changes in enthalpy or entropy.A classic way to isolate thymidylate synthase–negative mutants of bacteriais to treat a growing culture with thymidine and trimethoprim. Most ofthe cells are killed, and the survivors are greatly enriched in thymidylatesynthase–negative mutants.(a) What phenotype would allow you to identify these mutants?(b) What is the biochemical rationale for the selection? (That is, why are themutants not killed under these conditions?)(c) How would the procedure need to be modified to select mammalian cellmutants defective in thymidylate synthase?
- Mutations in the Galactose-1-Phosphate Uridyl Transferase Gene Can you use RFLP to identify this mutation?Why do E. coli cells with a defective lacZ gene fail to show galactoside permease activity after the addition of lactose in the absence of glucose?b. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Denote the point in the pathway in which each individual is blocked.
- a. Compounds A, B, C, and D are known to be intermediates in the pathway for production of protein E. To determine where the block in protein-E production occurred in each individual, the various intermediates were given to each individuals cel Is in culture. After a few weeks of growth with the intermediate, the cells were assayed for the production of protein E. The results for each individuals cells are given in the following table. A plus sign means that protein E was produced after the cells were given the intermediate listed at the top of the column. A minus sign means that the cells still could not produce protein E even after being exposed to the intermediate at the top of the column. Draw the pathway leading to the production of protein E.Wild-type E. coli cells can synthesize all 20 common amino acids, but some mutants, called amino acid auxotrophs, are unable to synthesize a specific amino acid and require its addition to the culture medium for optimal growth. Besides their role in protein synthesis, some amino acids are also precursors for other nitrogenous cell products. Consider the three amino acid auxotrophs that are unable to synthesize glycine, glutamine, and aspartate, respectively. Match each nitrogenous product with the mutant or mutants that would fail to synthesize it. Each mutant may fail to synthesize more than one nitrogenous product, and some nitrogenous products may fail to be synthesized by more than one mutant. glycine auxotrophs glutamine auxotrophs aspartate auxotrophs Answer Bank adenine nucleotides cytosine nucleotides guanine nucleotides uridine nucleotidesConsider the following simple regulatory pathways. Assume the full pathway is shown. A- E- B- F- C- G- D- 1 A H- 2 B || L You identify several null mutations (a complete deletion of the gene). For each mutant (indicated with a - sign), determine whether the final product (I, J, K or L) is inducible, uninducible, or constitutive. 3 C 4 D- [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] E [Choose ] F G I H || J K