Living modified organism (LMO) is defined as any living organism that possesses a novel combination of genetic material obtained through the use of modern biotechnology. Two LMOs that have obtained approval from FDA to be commercialized are the Hawaiian 'Rainbow' papayas and the AquAdvantage salmons. Discuss the advantages of Hawaiian 'Rainbow' papayas and the AquAdvantage salmons over their respective wild type.
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- AquAdvantage salmon is a genetically modified (GM) Atlantic salmon developed by AquaBounty Technologies. The Food and Drug Administration (FDA) has approved the company's application to allow the entry of this GM salmon into the United States food supply late last year. The FDA announced that: "AquAdvantage salmon is as safe to eat as any non- genetically engineered (GE) Atlantic salmon, and also as nutritious." However, opponents of genetically modified food are claiming that the AquAdvantage salmons could pose a serious environmental risk to the marine ecosystem if the engineered fishes escape from the farms. These transgenic salmons would be a big threat to the wild salmons. Justify this statement.Genetically modified foods are products produced from organisms that have had changes introduced into their DNA using the methods of genetic engineering. Golden rice, Flavr Savr tomatoes and Roundup Ready soyabeans are products of this technology. Discuss the advantages of these three genetically modified crops. Name ONE (1) strategy to produce transformed plant cells.It has been suggested that it would make the study of human diseases easier if cloned transgenic animals were produced that carried faulty versions of human genes (e.g., the gene that causes cystic fibrosis). a. Why would such animals be useful in medical research? : b. What ethical questions are raised by the creation of such transgenic animals?
- Agrobacterium-mediated plant transformation is a method used to create transgenic plants. Agrobacterium tumefaciens, a soil bacterium works exceptionally well as a natural genetic engineer. Describe briefly the creation of a transgenic plant using A. tumefaciens with illustration.As the leading scientist in a biomedical science laboratory, it is a requirement to give advice to your lab assistants when they are having problems with their experiments. What advice would you give to your assistants that are having the following problems: After performing a polymerase chain reaction (PCR) and agarose gel electrophoresis to confirm the presence of the C01 gene of 750bp. 2.1. They observe no band appearing on an agarose gel. What would be your conclusion? 2.2. They observe three bands of different sizes that resemble a smear on the gel. Advice 2.3. They observe a single band on the gel and conclude that the PCR product is an exact copy of the original template DNA. Would you support their condusion? Explain. 2.4. Explain how PCR can be used to detect infectious agents in diagnoses of diseases.Cystic fibrosis (CF) is a genetic disorder affecting a number of organs, including the lung airways, pancreas, and sweat glands. Mutations in both copies of the CFTR gene causes cystic fibrosis. Imagine that you have sweat gland samples from several Cystic Fibrosis patients (A-C) with unknown mutations in CFTR. You also have normal (+) sweat gland sample to use as a positive control. А В С А В С Choose which mutation would explain the RNA and protein results in A, B, & C: 1. Promoter/Regulatory mutation 2. Silent mutation 3. Missense mutation 4. Deletion mutation 5. Splice site mutation 6. Nonsense mutation RNA gel Protein gel
- Microarray hybridization is used mostly in transcript profiling or assaying DNA variation. Although the technology for establishing DNA microarrays was developed only recently, numerous applications have already been developed and their impact on future biomedical research and diagnostic approaches is expected to be profound. Give some examples of the practical use of this technique.explain the ethical concerns for this biotechnology method (sanger sequencing)Remember that although there are many interesting ideas about genetic engineering of plants and animals, this is specifically about GE bacteria. Please be sure you are answering the following questions: 1) What is the species that was modified, and what species did the introduced genes come from? 2) What is the purpose of the genetic engineering? What were people hoping to accomplish? 3) What are the benefits (or potential benefits) of the engineered bacterium? 4) What are the risks (or potential risks) of the engineered bacterium?
- pZERO®-1 is a 2808 bp cloning vector from Invitrogen. This vector allows effective selection of positive recombinants via disruption of the lethal gene, ccdB. Besides the lethal gene, it has an additional Zeocin resistance gene for tighter screening control. The Zeocin resistance gene in this vector is certainly more advantageous over ampicillin resistance gene. Give its THREE (3) advantages.Genetic information has become part of our culture and it is difficult to tell the difference between unmodified and genetically modified food sources such as plant and animals. After reading this module’s material regarding vectors in biotechnology, consider the potential for nanotechnology and scientific advancement.Research nanotechnology and its potential use in biotechnology. In one or two paragraphs, explain the potential advantages and disadvantages of nanotechnology in health care, agriculture, or industry and discuss whether you would or would not support further research.2) Transgenic animal refers to an organism whose genome has been altered by the transfer of a gene or genes from another species or breed. Cows, pigs and goats had been modified especially for pharmaceutical purposes. Explain three (3) most used transfection technology for the creation of transgenic animals.