Match the term to the application or description Synthetic Stem Cells 1. IPS Amplifying small amounts of DNA 2. Crispr Small circular DNA molecule Cutting purified DNA at specific sequences Making specific DNA alterations in living organisms 3. Restriction enzyme 4. PCR 5. Plasmid
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Genetics Question 3
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- Pcr is an twchnique to amplify: dna rRna mrna proteinCloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymeraseFor each of the following, give a brief description of the purpose and give an application Restriction enzymes Plasmids Gel Electrophoresis PCR
- SC DF eas fill The diagram below illustrates how recombinant DNA technology is used to produce valuable products. What is the purpose of using restriction enzymes in recombinant DNA technology? PDF UNOFFIC TRANSCR ostly cloudy DNA plasmids OO Bacterial cell (E. coli) Bacterium with recombinant DNA plasmid Plasmids isolated OO DO Cloned cells produce new protein such as enzymes or hormones O-C Ligase joins sticky ends Replication of new gene 00 00 Restriction enzyme cuts plasmids Sticky ends Gene obtained from donor DNA using the same restriction enzyme O The restriction enzymes are used to catalyze the synthesis of new proteins such as enzymes or hormones. Sticky ends O The restriction enzymes are used to join sticky ends of both the cut plasmid and the cut gene obtained from donor DNA PI mas omis O SearchFIGURE 2 shows the only suitable DNA restriction site in a plasmid DNA vector that can be cleaved. 5'.GTAATCCGGATCCGAAATCCCCCGG... 3' 3.CATTAGGCCTAGGCTTTAGGGGGCCC... 5 FIGURE 2 Identify the most suitable enzymeto be used as a restriction enzyme forthis purpose. O Ligase O Smal O EcoRI О ЕсoRVThe figure below illustrates the stages of production of useful medical products using recombinant DNA technology. Closely study the figure then answer the Activity 1 questions that follow: Human cell Bacterium 1. DNA Plasmid DNA Human insulin-producing gone 2. DNA is cut with restriction Bacterial DNA with human gone inserted enzymes. 3. Plasmid is reintroduced into bacterium. 4. Engineered bacteria multiply producing insulin. 5. Insulin is Human insulin separated and purified to produce human insulin. 6. Insulin injected into patient 1. What is a plasmid? Make a diagram of bacterial cell containing a plasmid. 2. How do you explain the selection of plasmids for carrying the desired gener 3. Follow the steps of human insulin production, then 4. Make a diagram for the production of growth hormone. first
- Give me 3 examples of how PCR and restriction enzymes can be used to create a genetic fingerprintWhich of the following is not a component of Sanger sequencing? OddNTPs with fluorescent tags Primers Restriction enzymes O DNA templateWhich is a complete list of the ingredients that are essential for PCR? nucleotides, DNA template, Taq polymerase, and plasmids nucleotides, DNA template, DNA ligase, and plasmids nucleotides, DNA template, Taq polymerase, and primers restriction enzymes, DNA template, Taq polymerase, and primers nucleotides, DNA template, DNA ligase, and primers
- The main enzymes commonly used in genetic engineering are: restriction endonuclease and ligase ligase and polymerase restriction endonuclease and polymerases O endonuclease and ligase________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by ___________ to then be replicated in the host. Group of answer choices 1. Restriction enzymes; ethidium bromide 2 DNA polymerases; ligase 3 Restriction enzymes; ligase 4 Ligases; restriction enzymesRegarding STR markers used in forensic science. Tick all the correct statements: no correct statement the PCR primers used to amplify STRs are located in the repeat units PCR primers to amplify STRs are located on both sides of the repeat units the PCR primers used to amplify STRs are coupled to a fluorochrome which is essential for the detection of amplicons they are absent from the gonosomes the allelic frequencies of STR markers vary according to the ethnicity of the individuals genotyped they are present homogeneously throughout the nuclear genome