Phosphorus in urine can be done by treating with molybdenum (VI) and then reducing the phosphomolybdate with aminonaphthol sulfonic acid to give the characteristic blue color. The wavelength of maximum absorption was at 690 nm. A patient excreted 1270 mL urine in 24 h, and the pH of the urine was 6.5. A 1.00-ml aliquot of the urine was treated with molybdate reagent and aminonaphthol sulfonic acid and was diluted to a volume of 50.0 mL. A series of phosphate standards was similarly treated. The absorbance of the solutions at 690 nm, measured against a blank, were as follows: alution

Phosphorus in urine can be done by treating with molybdenum (VI) and then reducing the phosphomolybdate with aminonaphthol sulfonic acid to give the characteristic blue color. The wavelength of maximum absorption was at 690 nm. A patient excreted 1270 mL urine in 24 h, and the pH of the urine was 6.5. A 1.00-ml aliquot of the urine was treated with molybdate reagent and aminonaphthol sulfonic acid and was diluted to a volume of 50.0 mL. A series of phosphate standards was similarly treated. The absorbance of the solutions at 690 nm, measured against a blank, were as follows: alution

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry

Section: Chapter Questions

Problem 13.8QAP: At 580 nm, which is the wavelength of its maximum absorption, the complex Fe(SCN)2+ has a molar...

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