Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I site, what can be expected about the growth coli transformants containing the recombinant plasmid? EcoRI Pstl Ampicillin resistance (AmpⓇ) BamHI Tetracycline resistance (TetⓇ) pBR322 (4,361 bp) Sall

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I
site, what can be expected about the growth coli transformants containing the recombinant plasmid?
EcoRI
Pstl
Ampicillin
resistance
(AmpⓇ)
Origin of
replication
(ori)
d. All of the above are true
pBR322
(4,361 bp)
Pvull
BamHI
Tetracycline
resistance
(TetⓇ)
Sall
a. The transformants should grow on nutrient agar containing ampicillin and tetracycline.
b. The transformants should be able to grow on nutrient agar without antibiotics.
c. The transformants should be able to grown on nutrient agar containing ampicillin
Transcribed Image Text:Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I site, what can be expected about the growth coli transformants containing the recombinant plasmid? EcoRI Pstl Ampicillin resistance (AmpⓇ) Origin of replication (ori) d. All of the above are true pBR322 (4,361 bp) Pvull BamHI Tetracycline resistance (TetⓇ) Sall a. The transformants should grow on nutrient agar containing ampicillin and tetracycline. b. The transformants should be able to grow on nutrient agar without antibiotics. c. The transformants should be able to grown on nutrient agar containing ampicillin
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