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Pour-Plate Method
1. Three milliliters instead of 1 mL was placed in the Petri dish.
Effect:
Rationale:
Step by step
Solved in 2 steps
- Principle of Methamine silver techniqueSerial dilution is recommended prior to pour and spread plate. Why is this so?Topic: Isolation of Crude Ovalbumin from Egg White by Ammonium Sulfate Precipitation (Salting Out) The computed amount of powdered ammonium sulfate was added to the egg white sample portion byportion with constant stirring while submerged in an ice bath. The solution is expected to become moreturbid, and a white precipitate is expected to form. The resulting mixture was then filtered using a cheesecloth. The residue was discarded, and 30.0 mL of thefiltrate was brought from 40% to 60% saturation by adding the required amount of powdered ammoniumsulfate in the same manner as the previous addition. After adding ammonium sulfate, the mixture was allowed to stand with occasional stirring for 30 minutes inan ice bath. The formation of a white precipitate is expected to happen QUESTION: Explain the significance of allowing the mixture to be submerged in an ice bath for a given time.
- what is the effect and rationale when three milliliters instead of 1 mL was placed in the Petri dish in pour-plate method?Cause-and-Effect Analysis: Given the conditions below, describe the effect on the staining procedure and explain briefly: 1. Excessive heat was applied during fixation 2. Low concentration of crystal violet used during gram staining 3. Excessive washing between steps 4. Insufficient decolorization 5. Excessive counterstainingUnknown Testing: Which compound(s) were present in the unknown sample? (sugar? starch? protein?
- Step-by-step smear preparation of Kinyoun MethodThe outer surface of the product container was not disinfected. effect: rationale:Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to tube 2, 4 ml to tube 3,6 ml to tube 4, 8 ml to tube 5 and 10 ml to tube 6. Mix thoroughly. 3. Transfer 1 ml of each into 6 separate test tubes (discard excess solution) and add 1 ml of 1% starch paste. Mix well and heat in a water bath with temperature maintained at 40°C for 30 minutes. 4. Divide each of the contents of each tube into 2 and test with: a. Iodine Test – to half of the content of each tube, add 1 drop of iodine in KI and note the color produced. Compare the intensity of color in each of the 6 tubes. b. Benedict's – to 1 ml benedict's reagent, add 5 drops of the other half of each tube and heat in a boiling water bath for 3 minutes. Note color of precipitate. 5. Rank each tube according to decreasing reaction rate one (1) being the fastest to hydrolyze and 6 being the slowest.
- PLAQUE 1. Using a toothpick, remove as much plaque as possible from between your molars. The sample will be placed into a mortar (be sure to get it the sticky plaque off of the toothpick). 2. Dispense 2 ml of the buffer into the mortar and grind the buffer-plaque with the pestle. Grind it up well. 3. You will make a 1/100 dilution (as near as we can, considering that we will not weigh out the plaque) of the plaque in phosphate buffer by adding the ground up plaque-buffer in the mortar back into the 99ml diluent buffer bottle and mixing it well . 4. Make 10 fold dilutions by using 9ml phosphate buffer solution dilutions. Be SURE to mix each dilution well, and use new pipettes. o Transfer 1ml of the 10-2 into 9ml phosphate buffer, using a fresh pipette: This is a 10 -3 . o Transfer 1ml of the 10-3 into 9ml phosphate buffer, using a fresh pipette: This is a 10-4. o Transfer 1ml of the 10-4 into 9ml phosphate buffer, using a fresh pipette: This is a 10-5. o Transfer 1ml of the 10-5 into…Procedure:1. Prepare 5 test tubes. Place 1 ml of 1% starch and add 10 drops of saliva toeach tube. Mix thoroughly.2. Place the first tube in ice water, the 2ndtube leave at room temperature, the 3rdtube in 40°C , the 4thtube at 60°Cwater bath and the 5thtube boil for 2minutes..3. Leave the 4 tubes in their respective temperatures for 30 minutes. The 4thtubeallows to stand for 30 minutes after heating for 2 minutes.4. Test the contents of each tube with iodine and benedict’s tests.1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to completely grind the beef? 3. Why is necessary to balance the homogenate tubes for centrifugation? 4. How do you prepare a 100mL of 0.1 M phosphate buffer? 5. From the anterior buffer, how do you make 100mL of 0.05 M? 6. Calculate the amount of ammonium sulfate necessary to get a 20% solution? 7. Which is the importance of dialysis?