Provide a chemical test/reagent that would differentiate each of the following pairs of compounds. Also, for each test, indicate the compound (write only the corresponding CAPITAL LETTER) that would give the positive result, and the indication(s) for a positive result. OH OH 1. and B А CEC-H and 2. A OH Но CH3 3. and A
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- Explain the chemical basis (reaction) of Millon’s test. (Generic reaction and explanation) What is the difference between Millon's test and Biuret test?(minimum 5)Discuss the medical application of the Benedict’s test? What other test(s) are used in parallel to Benedict’s test?Provide the Principles and detailed procedures of the following tests for the "Color Reaction of Proteins". 1. Hopkins-Cole Test 2. Sulfur Test 3. Heller's Test
- You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution; however, you only have access to two9 ml dilution blanks. Explain how you could accomplish this task with only two blanks.What is the phenol red test? Please explain the testing and its results and the chemistry behind it? Please explain for a beginners level. Thank youThe table below summarizes the results for Hopkins-Cole test. Provide the correct remarks from the results if Negative for Hopkin's Cole test or Positive for Hopkin's Cole test and if Negative for indole group or Has the presence of indole group.
- The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…To prepare a vaginal irrigation, a nurse uses 3 tsp of vinegar and 1.5 L of water. a. How many ml of vinegar should she use? b. If she prepares a solution using only 500 ml of water, how many ml of vinegar should she use?What does translucent spot mean with regards to lipid? Identify the control for this test. 1. Oil 2. Cheese 3. Water 4. Cheese
- The table below summarizes the results for Lead-sulfide test. Provide the correct remarks from the results if Negative for Lead-sulfide test or Positive for the Lead sulfide test and it's either Negative for -SH or Positive for -SHYou need to make a 1/750 dilution of each human serum sample prior to testing it in your ELISA. You will need 1 ml of the diluted serum (which already includes extra for Murphy's Law.) Calculate the volume of undiluted human serum you need for your 1 ml test volume. 1.3 microliters O 13 microliters O 0.75 microliters O 7.3 microlitersParaphrase the text below: Series of test tubes were filled with the desired volume of the BSA (0.1, 0.2, 0.3… 1 ml) .PBS was added to this to make the volume of 1 mL. 5 mL of copper reagent was added to all the tubes. After proper mixing, all the tubes were incubated at room temperature for 15 minutes. 1 mL Folin reagent was added to each and mixed properly with the help of vortex mixer and incubated for 20 minutes. The intensity of the colour was then determined spectrophotometrically at 680 nm. The graph was than plotted between optical density and the amount of BSA. Proteins estimation was done for the detection for the amount of proteins present in the sample solutions by the Lowry method