Q24) Consider the following data obtained from the size exclusion chromatography of polypropylene, which was made from propylene of molecular weight 42.08 g/mol. S.No. Number of Molecular Weight Molecules Range (g/mol) 8,000-16,000 16 16,000-24,000 3. 24 24,000-32,000 4. 28 32,000 – 40,000 20 40,000-48,000 48,000-56,000 From the above data, determine a) Number average molecular weight (M). b) Weight average molecular weight ( Mw).
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- Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.ef Correlate the structures of the standard food dyes to their relative position in the paper chromatogram. Refer to the structures shown below. H3C. *Na O O HO. O CH3 Allura Red AC (pigment in red dye) 01510 106 FO O Nat O Nat O Nat FO O Na+ 0=8=0 HO 0=S=0 Na Na+ Tartrazine (pigment in yellow dye) Brilliant Blue FCF (pigment in blue dye) What are the identities of the unknowns in paper chromatography of food dyes? How did you arrive at this conclusion? Differentiate normal phase chromatography from reverse phase chromatography. From which of the two governs the experiment you did in thin layer chromatography of plant pigments? How do you say so?Based on the given chromatograph, answer the following questions:1) Which of the diet sodas have the highest concentration of caffeine? Which standard best reflects this concentration?2) Which of the diet sodas have the lowest concentration of aspartame? Which standard best reflects this concentration?3) What is the approximate retention time of benzoate?
- Column Chromatography Alumina Chromatography Mixture 9:1 Hexanes:Ether 8:2 Hexanes:Ether 1:1 Hexanes:Acetone Amount Used 3.962 g 0.143 g 9.50 mL 9.50 mL 11.00 mL Additional Observations (Color, etc.) BIU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BI IU X₂ X² → BIU X₂ X² →A student analyzed a mixture of amino acids using thin layer chromatography where the stationary phase is a silica-coated plate. For the mobile phase, he used 50% ethyl acetate in hexane. The mixture contained three (3) amino acids whose structures are shown below. OH Hollo HO OH OH OH NH₂ NH₂ NH₂ Threonine Valine Glutamic acid a. Identify spots A, B, and C in the developed TLC plate below based on the structures of the amino acids above (ie. which spot is threonine, valine, glutamic acid?). Hint: determine how the structures of the given amino acids differ A. BO С. b. Threonine can be phosphorylated using an enzyme called protein kinase. Given the same conditions the student used in his analysis, will the phosphorylated threonine have a higher, smaller, or the same Rf value compared to threonine? Which of the following compounds would have the lowest Rf value in a TLC analysis? A. Hexane-2,3-diol B. Benzene C. Diethyl ether D. 1,3,5-cyclohexanetriolA student performed a chromatography experiment to determine the Rf value of an unknown amino acid. The distances traveled by the amino acid and the solvent line were 3.0 cm and 8.5 cm, respectively. What is the Rf value for this amino acid? 0.3 cm O 0.35 1.8 5.5 0.85 cm 0.65 O 2.8
- 1a) Hexane, CH3(CH2)4CH3, is found to have a long retention time when separated from a mixture. A column with a C18 (18-carbon chain) stationary phase and a methanol-water modal phase is used for the separation. Explain why hexane has a long retention time. 1b)You wish to chromatographically seperate a mixtureof the follwing compunds. Which of the two compounds will be most difficult to seperatate from one another? Why?How does the chromatogram change when methanol:water (90:10) is used as the mobile phase compared to a 100% methanol run?Qno1: why there is a need of chromatography and chromatography thecniqs. B) when and where we should use normal and reverse phase chromatography
- Which one of the following chromatographic techniques is useful in the identification of organic molecules having molecular weights more than 20,000? 1) reverse phase chromatography 2) normal phase chromatography 3) high pressure liquid chromatography 4) gel permeation chromatography1) Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. STAAT 61 1.11 227 RT TYPE AREA XXXX XXXX XXXX AREAS 0.009 55874 44.117 ARIHT 0.61 XX XX XX 1.11 2.27 XX XX XX TOTAL AREA=XX MUL FACTOR=XX 1. What is the retention time of compound A? Compound B? 2. Which compound is present in a larger amount? 3. Which compound has the lower boiling point? 4. What would happen to the retention times of compounds A and B if the column temperature were raised? 5. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?Chemistry Briefly describe the differences between the scanning and SIM approaches with reference to your data. Discuss how and why your scanning (TIC) and SIM (mass chromatograms look different. Pay particular attention to selectivity of compounds detected and signal-to-noise. What are some advantages of SIM? What are some disadvantages?