QUESTION 1You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of theseproteins are shown in the table belowProteinAlcohol dehydrogenaseCarbonic anhydraseInsulin B chainPhosphorylase BGlutamic dehydrogenaseB-galactosidaseConcentration of ammonium sulfate(AS) required for precipitationMolecular Weight (kDa) Isoelectric point (pl)45%383.780%65%20%30%45%284.845.3986.8.499.51155.3You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, thencentrifuge to generate a supernatant (AS-S) and pellet (AS-P).What concentration of AS will you use to precipitate ẞ-galactosidase?20%30%45%65%O 80%

Proteins fold in a way where the hydrophobic residues are sequestered towards the interior of the…

Answered: QUESTION 1 You have a crude lysate…

Biochemistry

6th Edition

ISBN:9781305577206

Author:Reginald H. Garrett, Charles M. Grisham

Publisher:Reginald H. Garrett, Charles M. Grisham

Chapter18: Glycolysis

Section: Chapter Questions

Problem 20P: Understanding the Mechanism of Hemolytic Anemia Genetic defects in glycolytic enzymes can have...

See similar textbooks

Similar questions

You have a crude lysate sample (CL) containing a mixture of six proteins (1, 2, 3, 4, 5, ẞ- galactosidase), and your goal is to obtain purified ẞ-gal. Some characteristics of these proteins are shown in the table below. Protein Alcohol dehydrogenase Carbonic anhydrase Insulin B chain Phosphorylase B Glutamic dehydrogenase B-galactosidase 45% Concentration of ammonium sulfate (AS) required for precipitation Molecular Weight (kDa) Isoelectric point (pl) 38 3.7 80% 65% 20% 30% 45% 28 4.8 4 5.3 98 6.8 49 9.5 115 5.3 You begin your purification by performing an ammonium sulfate (AS) precipitation. You add the appropriate concentration of AS to your CL sample, incubate overnight at 4°C, then centrifuge to generate a supernatant (AS-S) and pellet (AS-P). What concentration of AS will you use to precipitate Glutamic dehydrogenase? © 20% O 30% 45% 65% 80%
asap
There are eighteen (18) rows of boxes, for around 36-38 boxes total. Please see images attached. This is a pathway trace, with the final box showing "ribose 5 phosphate" as the end product. Any help is appreciated-thank you! glutamate to ribose 5 phosphate No a ketoglutarate dehydrogenase No pyruvate carboxylase No transketolase No transaminase You do get to have if needed: erythrose 4 phosphate asparate dihydroxyacetone phosphate bicarbonate ATP ornithine CO2
A sample of glycogen from a patient with liver disease is incubated with orthophosphate, phosphorylase, the transferase, and the debranching enzyme ( α -1,6- glucosidase). The ratio of glucose 1- phosphate to glucose formed in this mixture is 100. What is the most likely enzymatic deficiency in this patient?
Lactate dehydrogenase is a tetramer of MW 134000 g/mole composed of subunits which are equal in size. It is found in the cytoplasm of eukaryotic and prokaryotic cells. Describe the secondary, tertiary and quaternary structures of lactate dehydrogenase.
Which of these enzymes is needed to maintain a steady pool of methylene THF used in pyrimidine synthesis and cancer cells proliferation? Group of answer choices Phosphoglyceratedehydrogenase Glyceraldehydegehydrogenase Glycine decarboxylase Thymidylatesynthase
Please answer the following questions 1. Give the substrate for each of the following enzymes: a. dihydrofolate reductase b. acetylcholinesterase c. lactate dehydrogenase d. DNA polymerase e. aspartate transaminase
Discuss the importance of redox reactions in the metabolism of glucose. Be sure to address nicotinamide adenine dinucleotide in your answer.
Based on the image below, select the correct statement HN Aspartate Rib-P Inosinate (IMP) IMP H₂O OOC-CH₂- COO NH Fumarate N 274 adenylosuccinate lyase Rib-P Adenylosuccinate GTP GDP + P₁ adenylosuccinate IMP synthetase NAD+ NADH + H+ dehydrogenase Figure 22-36 Lehninger Principles of Biochemistry, Sixth Edition ©2013 W. H. Freeman and Company H HN H -N Gin Rib P Xanthylate (XMP) NH₂ Rib P Adenylate (AMP) Glu ATP AMP + PP₁ XMP-glutamine amidotransferase HN H₂N O GTP is phosphorylated in the conversion of IMP to GMP GTP is phosphorylated in the convertion of IMP to AMP GTP is dephosphorylated in the conversion of IMP to GMP O GTP is dephosphorylated in the conversion of IMP to AMP Rib-P Guanylate (GMP) GMP
Describe the biochemical role of coenzyme Q10 and how supplementation may impact metabolism. Be sure to address the key terms listed below as part of your discussion; you may address additional etiologies as well. coenzyme Q Complex I Complex II Complex III ubiquinone ubiquinol Electron transport chain Oxidative phosphorylation Reactive oxygen species
Outline the mechanism of the conversion of a-ketoglutarate to succinylCoA catalyzed by α-ketoglutarate dehydrogenase complex. Include all products, coenzymes, and reactions in your discussion.
Question 1 Which of the following statements is true regarding compounds of the TCA cycle resulting from introduction into the TCA cycle of Glutamate labeled in the R group carboxyl carbon transaminated to a-Ketoglutarate and taken through one complete round back to Succinyl CoA? Multiple answers: Multiple answers are accepted for this question Select one or more answers and submit. For keyboard navigation. SHOW MORE V a After one complete round Succinyl CoA will be radioactive. b The labeled R group carboxyl of Glutamate will be released as radioactive carbon dioxide. The transfer of Acetyl-CoA to Oxaloacetate will be result in radioactive Citric Acid. d A possible source of radioactive carbon dioxide is the Isocitrate Dehydrogenase reaction. e A possible source of radioactive carbon dioxide is the a-Ketoglutarate Dehydrogenase reaction. f No released carbon dioxide is radioactive. If taken through two complete rounds Succinyl CoA produced will be radioactive. h The carbon of the…

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

SEE MORE TEXTBOOKS