Regarding Gram staining, what will be the appearance of the Gram-negative bacteria if... All steps are done correctly? [Choose ] [Choose Pink The slide is not heat-fixed prior to Purple staining? Clear Orange Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? Not enough acetone:alcohol was used? Carbol fuschin was omitted? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 2

Regarding Gram staining, what will be the appearance of the Gram-negative bacteria if... All steps are done correctly? [Choose ] [Choose Pink The slide is not heat-fixed prior to Purple staining? Clear Orange Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? Not enough acetone:alcohol was used? Carbol fuschin was omitted? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 2

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 6RQ

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izzes/00071/take/questions/600381 Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? The slide is not heat-fixed prior to staining? Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? I Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? W [Choose ] [Choose ] Purple Clear Pink Orange [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] 88 22°C
Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 X
Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.
Table 1. Different Staining Techniques Gram Stain Acid Fast Endospore Capsule Flagella 1. Principle 2. Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) 3. Stain/s Used 4. Type of Stain 5. Mechanism of Staining stain used) (of each 6. Flow of Method
1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?
Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test
Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please explain, thank you.
Out of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test. Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are: Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis
Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. Gram positive Gram negative 2. Mordant was skipped Gram positive Gram negative 3. Decolorizing step was omitted Gram positive Gram negative 4. counterstain was not used? Gram positive Gram negative 5. Crystal violet and safranin was swapped in position. Gram positive Gram negative
What stain technique must this be? What is used to stain the background? What is used to stain the bacterial cell (in our method)? What structure is the clear (white) layer? Do we heat fix this? Capsule stain, No heat fixation, crystal violet, copper sulfate,
Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
What color are Gram negative cells after decolorizing with ethanol? What is the best age for your culture when performing a Gram stain? Why? Why is Gram staining considered a differential staining process? What are the primary and counterstain? What is the purpose for using a counterstain? What are some reasons a Gram positive cell might appear Gram negative? What color would a Gram negative cell be if you forgot to wash with ethanol and why? What color would a Gram negative cell be if you forgot to stain it with safranin and why?