Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? [Choose ] [Choose ] Purple The slide is not heat-fixed prior to Clear staining? Pink Orange Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]

Regarding Gram staining, what will be the appearance of the Gram-positive bacteria if... All steps are done correctly? [Choose ] [Choose ] Purple The slide is not heat-fixed prior to Clear staining? Pink Orange Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? Not enough acetone:alcohol was used? Carbol fuschin or safranin was omitted? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.4: The Hemacytometer

Problem 1CT

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66671/take/questions/800382 Regarding Gram staining, what will be the appearance of the Gram-negative bacteria if... All steps are done correctly? [Choose ] [Choose ] Pink The slide is not heat-fixed prior to Purple staining? Clear Orange Crystal violet was omitted? lodine was omitted? Only iodine was used? Acetone:alcohol was omitted? Too much acetone:alcohol was used or was left on for too long? Not enough acetone:alcohol was used? Carbol fuschin was omitted? Carbol fuschin or safranin was omitted? [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ] [Choose ]
Why is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 X
Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. Gram positive Gram negative 2. Mordant was skipped Gram positive Gram negative 3. Decolorizing step was omitted Gram positive Gram negative 4. counterstain was not used? Gram positive Gram negative 5. Crystal violet and safranin was swapped in position. Gram positive Gram negative
Out of twelve tests that can be performed: Gram Stain Wet Mount Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test. Which four tests are the best fit to help identify bacteria that is unknown? Possible bacterias that are being observed are: Enterobacter aerogenes Escherichia coli Micrococcus luteus Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis
Table 1. Different Staining Techniques Gram Stain Acid Fast Endospore Capsule Flagella 1. Principle 2. Characteristic of Bacteria viewed using this stain. (e.g. bacterial type, strain, or targeting particular bacterial feature/s) 3. Stain/s Used 4. Type of Stain 5. Mechanism of Staining stain used) (of each 6. Flow of Method
indicators, key used for/ determines reagents, or key positive result ingredients 1. crystal violet Comments/ additional info Medium/test negative result decolorization is key. Must include +/- controls on gram reaction and size and 2. iodine morfdant Gram stain stains purple stains pink 3. gram's declorizer 4. safranin shape every slide to verify result quadrant streak- isolation of pure culture of bacteria check this for any pigmentation of colonies TSA Eosin methylene blue agar (EMB) 1. sulfur/H2S 2. indole/tryptophasnase 3. motility/flagella 1. blackening of medium 2. red color in added reagent 3. fuzzy appearance of 1. no blackening of medium 2.same color of added multi test medium (3 results reagent (not red) 3. stab is visible an defined with growth 1. ferrous salts 2. Kovac's SIM in one medium) 3. low % agarose medium/stab not visible motility agar Brewer's plate in Anaerobe jar Fluid thioglycollate (FTM) Citrate slant phenol red fermentation broth test media (list the specific…
Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test
t/Root/Pages/TestTaker/MonitorLaunch.aspx?id%3DE9B80AE3-962E-4628-B79D-A3F06F1CAB72 MonitorLaunchaspx?id%3DE9B80AE3-962E-4628-879D-A3F0EFICABT2-Google Chrone uninoculated tube The medium shown here was inoculated with a bacterium then incubated for 48 hours; an uninoculated control tube is also present for comparison. This particular bacterium gave a for this test. a) O negative result b) O positive result c) O indeterminate result Review Later d) O variable result
Which of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.
Write the process/steps in Preparation of Bacillus atrophaeus culture using Blood agar plates and MacConkey agar plates. Note: This will perform the students so please write it in the easiest manner. Thanks
Out of all these tests, which 2 would help further understand this unknown lab. (Gram Stain and Growth Result is already done) Possible Tests (pick 2 that would help know the unknown bacteria) Gram stain morphology & arrangement color and growth characteristics on agar 37C, 30C and 25C (room temp) CAMP test starch hydrolysis test bacitracin susceptibility test glucose, lactose, sucrose, maltose fermentation tests methyl red/MR test Mannitol MacConkey Phenol Red Vogues-Proskauer/VP test citrate utilization test urea hydrolysis test phenylalanine deaminase test SIM test (Sulfide/Indole/Motility) Nitrate reduction test Oxidase test Catalase test
Types of Differential Staining techniques. 1. Gram stain 2. Acid-fast stain Please explain, thank you.