Transcriptional activator proteins are modular (a) The complete Gal4 dimer Activation domain Gal4 DNA-binding domain lacz ON Gal4 site (b) Gal4 lacking the activation domain lacz OFF Gal4 site (e) LexA lacking the activation domain DNA-binding, domain lacz OFF LexA site (d) Gal4-LexA hybrid Gal4 activation domain- LexA DNA-binding domain lacz ON LexA site AL structural FIGURE 12-7 Transcriptional activator proteins have multiple, separable domains. (a) The Gal4 protein has two domains and forms a dimer. (b) The experimental removal of the activation domain shows that DNA binding is not suficient for gene activation. (c) Similarly, the bacterial LexA protein cannot activate transcription on its own, but, when fused to the Gal4 activation domain (d), it can activate transcription through LexA-binding sites. AL structural se and ATP, it n turn causes iption factors 3, Gal80, and or absence of onal regulator on and bacte-

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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In any experiment, controls are essential in order to determine the specific effect of changing some parameter.
In Figure 12-7, which constructs are the “controls” that
serve to establish the principle that activation domains
are modular and interchangeable?

Transcriptional activator
proteins are modular
(a) The complete Gal4 dimer
Activation
domain
Gal4
DNA-binding
domain
lacz
ON
Gal4 site
(b) Gal4 lacking the activation domain
lacz
OFF
Gal4 site
(e) LexA lacking the activation domain
DNA-binding,
domain
lacz
OFF
LexA site
(d) Gal4-LexA hybrid
Gal4 activation
domain-
LexA
DNA-binding
domain
lacz
ON
LexA site
AL structural
FIGURE 12-7 Transcriptional activator
proteins have multiple, separable domains.
(a) The Gal4 protein has two domains and
forms a dimer. (b) The experimental
removal of the activation domain shows
that DNA binding is not suficient for gene
activation. (c) Similarly, the bacterial LexA
protein cannot activate transcription on its
own, but, when fused to the Gal4
activation domain (d), it can activate
transcription through LexA-binding sites.
AL structural
se and ATP, it
n turn causes
iption factors
3, Gal80, and
or absence of
onal regulator
on and bacte-
Transcribed Image Text:Transcriptional activator proteins are modular (a) The complete Gal4 dimer Activation domain Gal4 DNA-binding domain lacz ON Gal4 site (b) Gal4 lacking the activation domain lacz OFF Gal4 site (e) LexA lacking the activation domain DNA-binding, domain lacz OFF LexA site (d) Gal4-LexA hybrid Gal4 activation domain- LexA DNA-binding domain lacz ON LexA site AL structural FIGURE 12-7 Transcriptional activator proteins have multiple, separable domains. (a) The Gal4 protein has two domains and forms a dimer. (b) The experimental removal of the activation domain shows that DNA binding is not suficient for gene activation. (c) Similarly, the bacterial LexA protein cannot activate transcription on its own, but, when fused to the Gal4 activation domain (d), it can activate transcription through LexA-binding sites. AL structural se and ATP, it n turn causes iption factors 3, Gal80, and or absence of onal regulator on and bacte-
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