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Was your column chromatography separation of the three compounds in clove oil successful? Explain why or why not.

Was your column chromatography separation of the three compounds in clove oil successful? Explain why or why not.

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene. но eugenol eugeoal acetate B-caryophyllene a) Rank the compounds from least polar to most polar b) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the following TLC results. Identify the compound that corresponds with each spot. 5 cm 2,8 cm c) Calculate the Rr value of the middle spot. Show your work d) What would happen to the Rr of the spots if you increased the polarity of the mobile phase? Explain e) When separating the compounds by column chromatography, which compound would you expect to elute first? Which compound would you expect to elute last? f) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8, one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with the fraction that they contain (1-4, 5-8, 9-12, 13-16). -+-F-F-F- --+------ ++-F-F- -++-F-F- g) Was your column chromatography separation of the three compounds in clove oil successful? Explain why or why not.
Transcribed Image Text:3. The main components of clove oil are eugenol, eugenol acetate, and b-caryophyllene. но eugenol eugeoal acetate B-caryophyllene a) Rank the compounds from least polar to most polar b) After analyzing clove oil by TLC using a 10% acetone in hexanes mobile phase, you obtain the following TLC results. Identify the compound that corresponds with each spot. 5 cm 2,8 cm c) Calculate the Rr value of the middle spot. Show your work d) What would happen to the Rr of the spots if you increased the polarity of the mobile phase? Explain e) When separating the compounds by column chromatography, which compound would you expect to elute first? Which compound would you expect to elute last? f) You perform column chromatography on a sample of clove oil and collect a total of 16 fractions. You analyze each fraction by TLC using four TLC plates. One plate has fractions 1-4, one has fractions 5-8, one has fractions 9-12, and one has fractions 13-16. After developing all of your TLC plates, however, you realize that you forgot to label the TLC plates with the fraction numbers. Label the TLC plates below with the fraction that they contain (1-4, 5-8, 9-12, 13-16). -+-F-F-F- --+------ ++-F-F- -++-F-F- g) Was your column chromatography separation of the three compounds in clove oil successful? Explain why or why not.
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