Do you expect the more polar component of the mixture to elute first or last during column chromatography? Why? Be sure to comment on any important intermolecular interactions.
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- What are the general characteristics of Gas Chromatography, Thin Layer Chromatography, Ion Exchange, HPLC, SEC and Affinity Chromatography? What elutes first? Additionally can you explain what exactly is stationary phase and mobile phase?What is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly answer at your own words. Answer should be to the point (4-5 lines).What is the role of specific visualizing agents in a Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum ).
- What are the disadvantages of uses the Thick layer stationary phase in Thin layer Chromatography (TLC)? Please shortly answer at your own words. Answer should be to the point ( specific 4-5 lines).What is meant by retention time in gas chromatography. In what order will they come hexane, ethyl acetate, 2-propanol if I use polar column? Why?Brief discussion of column chromatographic separation (solvent polarity, elution ofcompounds etc.)
- (7) Answer the following questions with the choices (a-d): (a) "Affinity" (b)"Adsorption" (c)"Size-exclusion" (d) "Partition"The best choice of mobile phase in a thin layer chromatography experiments is: Group of answer choices The most volatile solvent possible. The least polar solvent possible. The most polar solvent possible. A mixture of solvents that takes into consideration the polarity of the analyte.In normal-phase chromatography, the stationary phase is Therefore, the eluent strength of the solvent increases as the solvent becomes less) polar, because the solute is more soluble in the mobile phase as the polarity of the mobile phase -Energy (polar / non-polar). (increase / decrease). Circle the peak in the spectrum below that corresponds to the circled transition shown in the energy level diagram. E₁ ⇒ 2 (more / 1 (s
- In chromatography, the compound that is least soluble in the mobile phase a) Travels the furthest away from the line of origin b) Stays closest to the line of originGiven below is a diagram of separation of two components of a mixture in a reverse phase chromatographic column. From the diagram, which component is the most non-polar?Thin Layer Chromatography. All types of chromatography have mobile phaseand stationary phase. What is a stationary phase and a mobile phase? Elaborate your answer.