Answered: What is the statistically significant…

What is the statistically significant number range of colonies that may be counted on a Petri dish (for the pour plate method)?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter6: Basic Clinical Chemistry

Section6.2: Chemistry Specimen Collection And Processing

Problem 10RQ

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Concept explainers

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."

Question

1. What is the statistically significant number range of colonies that may be counted on a Petri dish (for the pour plate method)?

2. Scientific Notation. Fill in the missing information.

a) 4.5 x 10⁹ = _______

b) 50 x 10⁷ = _______

c) 2300 x 10¹⁰ = _______

d) 0.54 x 10⁸= _______

3. You wish to determine viable counts on a culture of Bacillus subtilis. You begin by pipetting 1 ml of culture into 99 ml of sterile water. After mixing the dilution well you make a series of 4 further dilutions of 10^-1 each. From the three most dilute samples, you prepare three pour plates using 1 ml in each. After incubation you find the plate counts of the plates are 16, 245 and 890 respectively.

a) Show the dilution scheme.

b) What is the estimated viable count (cells/ml) in the original culture?

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