What percentage of Vmax is obtained when the substrate is present at 80% of the KM? Use two digits in your answer. %
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- The Michaelis-Menten constant can be determined using the Lineweaver-Burk plot and is equal to twice the Vmax. can be determined using the Lineweaver-Burk plot and is equal to the substrate concentration at Vmax/2. can be determined using the Lineweaver-Burk plot. O is equal to the substrate concentration at Vmax/2. O is equal to twice the VmaxA microorganism is grown in a fermenter under steady-state conditions. The microorganism growth follows Monod kinetics and the following stoichiometric equation: C3H6O3 +a O2 +b NH3 → c biomass + d CO2 + e H₂O Given that the biomass yield is 0.415 grams of biomass per gram of substrate, determine the respiratory quotient.Lactic acid is produced by using molasses in stirred tank bioreactor . Thermophilic bacteria (45-50 0C) are used during production. Accordingly, how is 1 L of lactic acid produced in this production process? Draw bioreactor and flowchart.
- An aerobic culture is grown in a continuous bioreactor at 28C and the product of yield was found to be 4 grams product produced per gram of oxygen consumed. It is planned to make this product at a rate of 6 kg/h. Calculate the overall heat of reaction per hour (in kJ/hr).Nicotiana tabacum cells are cultured to produce a polysaccharide gum. The reactor used is a stirred-tank reactor with an initial volume of 100 L. The maximum specific rate of growth of the culture is 0.18 d-1 and the yield coefficient of substrate in biomass is 0.5 gX/gS. The concentration of the limiting substrate in the medium is 3% (m/v). The reactor is inoculated with 1.5 g/L of cells and operated in batch until the substrate is exhausted, when the medium is fed with a constant flow rate of 8 L/d. The fed batch occurs in a quasi-steady state condition.a) Estimate the time of the batch step and the concentration of cells reached in this phase, considering exponential cell growth.b) The fed batch phase is carried out for a period of 40 days. What is the final concentration of cells in the reactor?c) The bioreactor is available for the process for 275 days a year, with an interval of 24 hours between each cultivation. What is the most advantageous operating mode for the process…The growth of a bacterium follows the Monod model with μmax=0.3 h-1, Ks=2.5 g/l and Yx/s=0.20gx/gs. Calculate the number of continuous mixing tank type bioreactors in series, with 10 L of useful capacity each, necessary for the limiting substrate conversion to be greater than 98%, considering that the system is fed with a flow rate of 2.5 L/ h and the substrate concentration in the feed is 30 g/L.
- During cell couting, when the data are plotted in an ordinary graph or on a cross-section paper, why is the log value of the cell concentration used?Lactic acid is produced using molasses in a stirred tank bioreactor. Thermophilic bacteria (45-50 0C) are used during production. According to this a-) Calculate how 100 ml of lactic acid is produced in this production process. b-) Calculate how much raw material (molasses) is required for the production of lactic acid in the Stirred tank bioreactor. Draw the bioreactor and flowchart.Part a) Which graph has the largest kcat? Please explain. Part b) Which graph has the tightest substrate binding? Please explain. Part c) Which graph has the largest specificity or efficiency constant? Please explain.
- The following data are given for a biogas digester suitable for the output of six cows. The volume of digester is 7.4 m3, the volume of gas holder is 2.9 m and the retention time is 21 days. Find the height of gas holder cm height of gas holder =-Lactic acid production is carried out in the stirred tank bioreactor. 100 kg molasses entered the production tank initially. Thermophilic bacteria (45-50 0C) are used during production. According to this, a-) Calculate how much lactic acid is produced in this production. b-) draw the bioreactor and its flowchart. (raw material = molasses, the organism used for production = Thermophilic bacteria)Lactic acid production is carried out in the stirred tank bioreactor. 100 mol glucose entered the production tank initially. E.coli are used during production. According to this, a-) Calculate how much lactic acid is produced in this production.b-) draw the bioreactor and its flowchart. (raw material = molasses, the organism used for production = Thermophilic bacteria)