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- http://vlab.amrita.edu/?sub=3&brch=73&sim=1628&cnt=1 Explain how the Kirby-Bauer method relies on diffusion of antibioticsEx. 17: Chemotherapeutic Agents Using the Antimicrobial Susceptibility Table on pg 62 in the lab manual, determine whether not the bacteria plated in this Kirby-Bauer test are resistant (R), intermediate (I), or susceptible (S). The bacterial species tested was Escherichia coli (Gram negative). Ciprofloxacin Diameter = 20 Streptomycin Diameter 30 Carbenicillin Diameter 17 + Erythromycin Diameter = 23 Novobiocin Diameter = 0 bacterial growth = disk with antibiotic (see labels) = no growthA nine-laboratory cooperative study was performed to evaluate quality control for susceptibility tests with 30-µg netilmicin disks [6]. Each laboratory tested three standard control strains on a different lot of Mueller-Hinton agar, with 150 tests performed per laboratory. For protocol control, each laboratory also performed 15 additional tests on eachof the control strains using the same lot of Mueller-Hinton agar across laboratories. The mean zone diameters for each of the nine laboratories are given in Table 6.11. *6.20 Provide a point and interval estimate (95% CI) for the interlaboratory standard deviation of mean zone diameters for each type of control strain, assuming each laboratory uses different media to perform the susceptibility tests. *6.21 Answer Problem 6.20 assuming each laboratory uses a common medium to perform the susceptibility tests. 6.22 Are there any advantages to using a common medium versus using different media for performing the susceptibility tests with…
- What are the differences between “O” and “H” antigen? Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.Differentiate Widal from Weil-Felix test. The strains of Proteus vulgaris are used in Weil-felix reaction. Explain why.What are two possible reasons for choosing a bacteriostatic treatment over a bactericidal one? Name the factors that can compromise the effectiveness of a disinfecting agent
- Based on the journal article “Interpretation of the Tube Coagulase Test for Identification of Staphylococcus aureus”, what should be the result to conclude the organism is S.aureus? When is it necessary to have supplemental tests done for the identification of S.aureus?Compare and contrast the method for determining the minimum inhibitory concentration (MIC) with the Kirby-Bauer disc diffusion test.What is the role of H2SO4 in Hopkins cole test?
- For a laboratory exercise of antibiotic susceptibility test in a disk-diffusion method. With reference to the table below, determine the antibiotic susceptibilities of different antibiotics against E.coli and S.aureus.How will you prevent the occurrence of the bacteria present in the Rapid Strep A Test?Not only is blood agar an enriched medium that can support fastidious organism growth, but it can also serve as a differential medium in the identification of streptococcal species because of the presence of red blood cells that can be destroyed by an organism's hemolysins. Looking at this photo, how could you describe the organism growing on the surface of this blood agar plate? Multiple Choice a) Gamma-hemolytic b) Alpha-hemolytic c) Non-hemolytic d) Beta-hemolytic